Study of the protein-bound fraction of calcium, iron, magnesium and zinc in bovine milk

Silva, Fernando V.; Lopes, Gisele S.; Nóbrega, Joaquim A.; Souza, G. B. de; Nogueira, Ana Rita A.

doi:10.1016/s0584-8547(01)00313-5

Cited by 35 publications

(19 citation statements)

References 14 publications

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“…Since the samples were injected without pretreatment apart from dilution, a considerable amount of ion could still be bound with casein [26]. Thus, the reliability of the CE procedure developed should be established against the solution part of the samples instead of by recovery of spiked metals to avoid the complication due to the unknown distribution of metals between their soluble and bound form in milk.…”

Section: Applicability Studymentioning

confidence: 99%

Separation and determination of metal cations in milk and dairy products by CE

2007

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To prevent casein adsorption and improve between-run repeatability, a CE procedure is developed for cation analysis in milk using a modified imidazole/alpha-hydroxyisobutyric acid (HIBA) BGE system to operate at pH 6 to match the pH of milk and elevate imidazole concentration to enhance its buffer action. The procedure is shown to produce a fast, economic and efficient method for cation separation in milk with only simple dilution. Upon direct hydrodynamic injection of diluted milk sample at 8 cm for 30 s in an uncoated column with a BGE consisting of 10 mM imidazole, 10 mM HIBA and 10% methanol at pH 6.0 under +18 kV, baseline separation was achieved for K(+), Na(+), Ca(2+), Mg(2+), Mn(2+), Cd(2+), Co(2+), Ni(2+)and Zn(2+). Agreeable results at 95% confidence level were obtained using CE and inductively coupled plasma-atomic emission spectrometry (ICP-AES) for milk samples after protein removal. Baseline-resolved peaks for essential minerals were obtained for fresh and non-refrigerated reconstituted milks. Long-term stability was demonstrated by repeated determinations without rinsing and improvement in repeatability was shown by rinsing with 60 mM SDS in BGE. Information on metal speciation useful for nutritional assessment was obtained from CE to complement the ICP methods.

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Section: Applicability Studymentioning

confidence: 99%

Separation and determination of metal cations in milk and dairy products by CE

2007

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“…Almost 90% of Zn binds to casein in mature milk, in contrast to just 60% in the colostrums [29]. In casein, Zn binds primarily to colloid calcium phosphate of casein micelles [30].…”

Section: Resultsmentioning

confidence: 98%

A Quantitative Analysis of Chromium and Zinc in Water and Raw Milk in Chittagong, Bangladesh

Akther¹,

Shahriar²,

Alam

et al. 2016

J. Sci. Res.

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The concentration of selected heavy metals (chromium and zinc) was detected and estimated in water and raw milk collected from Chittagong City of Bangladesh. A total of 25 water samples and 20 raw milk samples were collected from different farms situated in 10 locations of Chittagong, Bangladesh. Chromium content in water was obtained in the range from 0.2 µg/L to 13 µg/L. The highest level of chromium content in water was 13.068 µg/L. The content of chromium in raw milk was obtained in the range from 2 µg/L to 17 µg/L. The highest level of chromium in milk was 17.062 µg/L. Chromium concentrations in water and milk were below the maximum allowed limit. The concentration of zinc in water was in the range from 0.01 mg/L to 1 mg/L. The highest level of zinc in water was 1.024 mg/L. The concentration of zinc in raw milk was in the range from 0.6 mg/L to 0.9 mg/L. The highest concentration of zinc in water was 1.024 mg/L which is around the toxicological guidance value of zinc.

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“…Calcium phosphate nanoclusters are formed by sequestration of amorphous Ca within a shell of casein and are generally known as the micellar Ca phosphate (Smith et al, 2004). Nevertheless, studies on the partition of salts in milk have shown that about one-third of the Ca, half of the Pi, two-thirds of the Mg, and ninetenths of the citrate in bovine milk are diffusible through a semipermeable membrane or can be separated by ultrafiltration (Silva et al, 2001). Thus, one cannot rule out the potential formation of PPi (if the NTPPPH enzyme exists in milk) and the subsequent spontaneous formation of Ca-PPi.…”

Section: Potential Role Of Phosphatase Located In Mslm and Mfgm In Prmentioning

confidence: 99%

Milk Lipoprotein Membranes and Their Imperative Enzymes

Silanikove

Advances in Experimental Medicine and Biology

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There are two main sources of lipoprotein membranes in milk: the relatively well-defined milk fat globule membrane (MFGM) that covers the milk fat globules, and the much less attended lipoprotein source, in the form of vesicles floating in the milk serum. We challenge the common view that the milk serum lipoprotein membrane (MSLM) is secondly derived from the MFGM and present a different view suggesting that it represents Golgi-derived vesicles that are released intact to milk. The potential role of enzymes attached to the MSLM and MFGM is considered in detail for select ubiquitously expressed enzymes.

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Study of the protein-bound fraction of calcium, iron, magnesium and zinc in bovine milk

Cited by 35 publications

References 14 publications

Separation and determination of metal cations in milk and dairy products by CE

Separation and determination of metal cations in milk and dairy products by CE

A Quantitative Analysis of Chromium and Zinc in Water and Raw Milk in Chittagong, Bangladesh

Milk Lipoprotein Membranes and Their Imperative Enzymes

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