Milk Lipoprotein Membranes and Their Imperative Enzymes

Silanikove, Nissim

doi:10.1007/978-0-387-74087-4_5

Cited by 11 publications

(4 citation statements)

References 57 publications

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“…Tissue sections of mammary glands of mastitic animal revealed low or no activity of alkaline phosphatase. In accordance with our results, Silanikove andShapiro (2007) andEL sayed et al (2009) have shown that AP is located almost on the mammary epithelial cells apical membrane. This weak activity of AP in mastitic tissues may be related with deactivation of this enzyme owing to negative regulatory process of mammary gland as a result of negative effects of pathogens.…”

Section: Discussionsupporting

confidence: 93%

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Some Studies on Pasteurella Multocida as a Causative Agent of Mastitis in Dairy Cows and Ewes

Asfour¹,

Anwer²,

Nabeeh³

et al. 2016

Assiut Veterinary Medical Journal

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A total of 233 mastitic milk samples (151 cows and 82 ewes) and 90 mammary gland tissue samples (56 cows and 34 ewes) were collected from different Egyptian Governorates for P. multocida investigation as one of mastitis causing pathogens and its effect on the mammary gland tissues. The isolated P. multocida from clinical mastitic milk was slightly higher than that from subclinical form in both cows and ewes (15.3% versus 13.1% and 27.3% versus 26.7%, respectively) and it was isolated from the udder tissues of both cows and ewes with percentages of (17.85%) and (23.52%), respectively. Enzyme linked immunosorbent assay (ELISA) was used as confirmatory, rapid and reliable test for detection of P. multocida antibodies in the tested milk samples and they were detected in mastitic cow's and ewe's milk whey in (17.2%) and (34.2%), respectively. The antibiogram profile of P. multocida was studied for detection of its susceptibility to 15 different antibiotics to detect the drug of choice for its treatment. P. multocida isolated from cow's milk showed more resistance to various antibiotics than that isolated from ewe's milk. The DNA integrity of mammary gland tissue cells was detected using comet assay and the percentage of DNA damage was significantly elevated in case of P. multocida infected mammary gland (P<0.05). In addition, the histopathological findings of P. multocida infected udder showed focal and/or diffuse chronic lymphocytic mastitis with an extensive degeneration and necrosis of the alveolar epithelium as well as interstitial tissue. Most of the mammary alveoli were filled with basophilic bacterial colonies with bipolar bodies positively stained by methylene blue and Giemsa stains. Cytological evaluation was conducted on all udder tissue samples and 16/18 positive cases (88.8%) were correlated with their histopathological examination. Histochemically, tissue sections from infected udder showed weak or no alkaline phosphatase activity and density of protein staining. It was concluded that P. multocida should be considered as an important sharing etiological agent of mastitis in both cows and ewes (especially in ewes) and associated with significant histopathological alterations in the glandular tissue structure. ELISA was considered as a quick and reliable technique for detection of P. multocida infection in the mammary gland especially in the un-vaccinated farms beside the traditional cultural method. The cytological interpretation was quiet helpful in rapid screening of the mammary gland affections.

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Section: Discussionsupporting

confidence: 93%

“…This weak activity of AP in mastitic tissues may be related with deactivation of this enzyme owing to negative regulatory process of mammary gland as a result of negative effects of pathogens. The weak activity of alkaline phosphatase enzyme could be due to impaired milk secretory mechanism (Silanikove, 2008).…”

Section: Discussionmentioning

confidence: 99%

Some Studies on Pasteurella Multocida as a Causative Agent of Mastitis in Dairy Cows and Ewes

Asfour¹,

Anwer²,

Nabeeh³

et al. 2016

Assiut Veterinary Medical Journal

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“…Milk protein synthesis in mammary tissue is a complex mechanism under the influence of local and systemic hormones along with some other factors those affect milk yield (Khaliq andHussain et al, 2010). The weak AP activity and protein density that observed in this work in singly isolated S.aureus mastitic tissue sections were observed previously by Hassan (2004); Silanikove (2008);El-Sayed et al (2009); Hussain et al (2012c) and Hussain et al (2013) which attributed to the degenerative changes of mammary epithelium with connective tissue proliferation and impaired activity of endoplasmic reticulum that induced by microbial agents. These degenerated mammary cells encompassing the active cellular protein was substituted by the spread of connective tissue under the bacterial toxins effect which resulted in poor biosynthetic capacity of udder and decreases cellular differentiation.…”

Section: Discussionsupporting

confidence: 80%

Immuno-Histomorphometric Studies and the Effect of Some Antibiotic Alternatives on She-Camel Staphylococcus Mastitis

2018

Assiut Veterinary Medical Journal

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“…Nucleobases are the nitrogenous bases that are the basic units of the genetic code (adenine (A), cytosine (C), guanine (G), thymine (T), and uracil (U)). They are essential for cellular function, acting as mediators of chemical energy transfer, signal transduction, and growth regulators [356,357].…”

Section: Nucleotides and Nucleosidesmentioning

confidence: 99%

A Comprehensive Review of Bovine Colostrum Components and Selected Aspects Regarding Their Impact on Neonatal Calf Physiology

Silva,

Pereira

et al. 2024

Animals

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Colostrum contains macro- and micronutrients necessary to meet the nutritional and energy requirements of the neonatal calf, bioactive components that intervene in several physiological aspects, and cells and microorganisms that modulate the calf’s immune system and gut microbiome. Colostrum is sometimes mistaken as transition milk, which, although more nutritive than whole milk, has a distinct biochemical composition. Furthermore, most research about colostrum quality and colostrum management focuses on the transfer of maternal IgG to the newborn calf. The remaining components of colostrum and transition milk have not received the same attention, despite their importance to the newborn animal. In this narrative review, a large body of literature on the components of bovine colostrum was reviewed. The variability of these components was summarized, emphasizing specific components that warrant deeper exploration. In addition, the effects of each component present in colostrum and transition milk on several key physiological aspects of the newborn calf are discussed.

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Milk Lipoprotein Membranes and Their Imperative Enzymes

Cited by 11 publications

References 57 publications

Some Studies on Pasteurella Multocida as a Causative Agent of Mastitis in Dairy Cows and Ewes

Some Studies on Pasteurella Multocida as a Causative Agent of Mastitis in Dairy Cows and Ewes

Immuno-Histomorphometric Studies and the Effect of Some Antibiotic Alternatives on She-Camel Staphylococcus Mastitis

A Comprehensive Review of Bovine Colostrum Components and Selected Aspects Regarding Their Impact on Neonatal Calf Physiology

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