Study of the Peripheral Nerve Fibers Myelin Structure Changes during Activation of Schwann Cell Acetylcholine Receptors

Verdiyan, E E; Allakhverdiev, Elvin S.; Максимов, Г. В.

doi:10.1371/journal.pone.0158083

Cited by 11 publications

(11 citation statements)

References 16 publications

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“…RR modes of 1157 and 1521 cm −1 could be a significant marker to diagnose glioma and other CNS cancers. 48,53,54…”

Section: Identification Of Glioma Grades By Carotenoidsmentioning

confidence: 99%

“…Rich fatty acid RR peaks at 1438, 2850, and 2885 cm −1 are stronger than protein bands at 1005, 1667, and 2932 cm −1 in intensity. In a comparative study, 48 Verdiyan et al collected Raman spectra from the myelin sheath of sciatic nerve fiber tissue using 532 nm laser excitation and a 50-s exposure time and observed peak intensities at 1160, 1520, 2850, 2885, and 2935 cm −1 , respectively. The normal control spectrum is collected from the cancer margin of grade IV glioma, which is also called the negative margin of the cancer or the cancer-normal tissue interface [ Fig.…”

Section: Introductionmentioning

confidence: 99%

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Optical biopsy identification and grading of gliomas using label-free visible resonance Raman spectroscopy

Zhou

Liu

et al. 2019

J. Biomed. Opt.

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Glioma is one of the most refractory types of brain tumor. Accurate tumor boundary identification and complete resection of the tumor are essential for glioma removal during brain surgery. We present a method based on visible resonance Raman (VRR) spectroscopy to identify glioma margins and grades. A set of diagnostic spectral biomarkers features are presented based on tissue composition changes revealed by VRR. The Raman spectra include molecular vibrational fingerprints of carotenoids, tryptophan, amide I/II/III, proteins, and lipids. These basic in situ spectral biomarkers are used to identify the tissue from the interface between brain cancer and normal tissue and to evaluate glioma grades. The VRR spectra are also analyzed using principal component analysis for dimension reduction and feature detection and support vector machine for classification. The cross-validated sensitivity, specificity, and accuracy are found to be 100%, 96.3%, and 99.6% to distinguish glioma tissues from normal brain tissues, respectively. The area under the receiver operating characteristic curve for the classification is about 1.0. The accuracies to distinguish normal, low grade (grades I and II), and high grade (grades III and IV) gliomas are found to be 96.3%, 53.7%, and 84.1% for the three groups, respectively, along with a total accuracy of 75.1%. A set of criteria for differentiating normal human brain tissues from normal control tissues is proposed and used to identify brain cancer margins, yielding a diagnostic sensitivity of 100% and specificity of 71%. Our study demonstrates the potential of VRR as a label-free optical molecular histopathology method used for in situ boundary line judgment for brain surgery in the margins.

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“…RR modes of 1157 and 1521 cm −1 could be a significant marker to diagnose glioma and other CNS cancers. 48,53,54…”

Section: Identification Of Glioma Grades By Carotenoidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optical biopsy identification and grading of gliomas using label-free visible resonance Raman spectroscopy

Zhou

Liu

et al. 2019

J. Biomed. Opt.

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“…Activation of M2 receptor arrests cell cycle and promotes Schwann cell differentiation via upregulation of Sox10 and Krox 20 and downregulation of c‐jun , Notch‐1 and Jagged‐1 , and increased expression of myelin proteins (Uggenti et al, ). Moreover, activation of AChR on Schwann cells modifies the viscosity of the myelin membrane (Verdiyan et al, ).…”

Section: Introductionmentioning

confidence: 99%

Cholinergic signaling in myelination

Fields

Dutta

Belgrad

et al. 2017

Glia

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There is a long history of research on acetylcholine (ACh) function in myelinating glia, but a resurgence of interest recently as a result of the therapeutic potential of manipulating ACh signaling to promote remyelination, and the broader interest in neurotransmitter signaling in activity-dependent myelination. Myelinating glia express all the major types of muscarinic and nicotinic ACh receptors at different stages of development, and acetylcholinesterase and butyrylcholinesterase are highly expressed in white matter. This review traces the history of research on ACh signaling in Schwann cells, oligodendrocytes, and in the myelin sheath, and summarizes current knowledge on the intracellular signaling and functional consequences of ACh signaling in myelinating glia. Implications of ACh in diseases, such as Alzheimer's disease, multiple sclerosis, and white matter toxicity caused by pesticides are considered, together with an outline of major questions for future research. GLIA 2017;65:687-698.

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“…In following experiments, the changes in conformation of myelin lipids and carotenoids (the minor membrane component) were studied during the long-lasting nerve fibers exposure with pronase E. As described in detail previously [ 14 , 15 ], the Raman spectrum of nerve fiber myelin could be characterized by the presence of a set of intensive bands, related to phospholipid fatty acids and carotenoids ( Fig 5A and 5B ). About this, we used another laser excitation wave, which made it possible to reduce the contribution of carotenoid molecules from the RS and to study only the changes in fatty acids of phospholipids.…”

Section: Resultsmentioning

confidence: 83%

Study of myelin structure changes during the nerve fibers demyelination

2017

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Raman, NMR and EPR spectroscopy and electrophysiology methods were used to investigate the excitability and the packaging of myelin lipid layers and its viscosity during nerve exposure to pronase E. It was established that during exposure of nerve to pronase E the action potential (AP) conduction velocity and the Schwann cell (SC) (or myelin) water ordering increases, but the nerve myelin refractive index and internode incisions numbers decrease. This effect included two periods–short- and long-time period, probably, because the first one depends on SC protein changes and the second one–on the nerve fiber internode demyelination. It was concluded that high electrical resistance of myelin, which is important for a series of AP conduction velocity, not only depends on nerve fiber diameter and the myelin lipid composition, but also on the regularity of myelin lipid fatty acids and myelin lipid layer packing during the axoglial interaction.

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Study of the Peripheral Nerve Fibers Myelin Structure Changes during Activation of Schwann Cell Acetylcholine Receptors

Cited by 11 publications

References 16 publications

Optical biopsy identification and grading of gliomas using label-free visible resonance Raman spectroscopy

Optical biopsy identification and grading of gliomas using label-free visible resonance Raman spectroscopy

Cholinergic signaling in myelination

Study of myelin structure changes during the nerve fibers demyelination

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