Study of myelin structure changes during the nerve fibers demyelination

Rodionova, N. N.; Allakhverdiev, Elvin S.; Максимов, Г. В.

doi:10.1371/journal.pone.0185170

Cited by 8 publications

(6 citation statements)

References 23 publications

(33 reference statements)

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“…As the paranodal regions are stabilized by autotypic tight junctions, one possibility is that in the absence of PrP C , these are slightly destabilized, leading to paranodal outfoldings. Furthermore, disrupted axoglial junctions have been shown to cause ion channel dispersion with accumulation of potassium between the axon and Schwann cell, eliciting swelling of the Schwann cell and remodeling of the myelin, a phenomenon that fits with the observed pathology in the nerves from PRNP Ter/Ter goats.…”

Section: Discussionmentioning

confidence: 61%

Demyelinating polyneuropathy in goats lacking prion protein

et al. 2019

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Studies in mice with ablation of Prnp, the gene that encodes the cellular prion protein (PrP C ), have led to the hypothesis that PrP C is important for peripheral nerve myelin maintenance. Here, we have used a nontransgenic animal model to put this idea to the test; namely, goats that, due to a naturally occurring nonsense mutation, lack PrP C .Teased nerve fiber preparation revealed a demyelinating pathology in goats without PrP C . Affected nerves were invaded by macrophages and T cells and displayed vacuolated fibers, shrunken axons, and onion bulbs. Peripheral nerve lipid composition was similar in young goats with or without PrP C , but markedly different between corresponding groups of adult goats, reflecting the progressive nature of the neuropathy. This is the first report of a subclinical demyelinating polyneuropathy caused by loss of PrP C function in a nontransgenic mammal. K E Y W O R D Slipidomic, myelin, neuropathy, PRNP haplotype 2360 |

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Section: Discussionmentioning

confidence: 61%

Demyelinating polyneuropathy in goats lacking prion protein

et al. 2019

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“…Carotenoids play an important role in the antioxidant defense system in the healthy brain [ 60 ]. Carotenoids have been extensively studied and confirmed as a cancer biomarker in human organs using Raman, especially resonance Raman spectroscopy, to identify cancers of the skin, rectum/colon, lung, breast, and brain [ 42 , 61 , 62 , 63 ]. Johnson et al reported the important role of carotenoids, and particularly the presence of lutein and zeaxanthin, in normal brain tissue [ 64 , 65 , 66 ].…”

Section: Analysis and Resultsmentioning

confidence: 99%

“…Raman spectroscopy techniques have been used to study human bladder cancer, esophageal cancer, gastrointestinal cancer, cervical cancer, skin melanoma lesions, lung cancer, breast cancer, and brain tumors, not only ex vivo but also in vivo [ 15 , 16 , 19 , 20 , 21 , 22 , 22 , 23 , 26 , 27 , 28 , 31 , 33 , 36 , 37 , 38 , 39 , 40 ]. Besides solid tumors, Raman spectroscopy has also been used to detect vulnerable atherosclerotic plaques, atherosclerotic abdominal aortic tissues, and nerve tissues, and even the cerebrospinal fluid and serum of patients [ 41 , 42 , 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

A Handheld Visible Resonance Raman Analyzer Used in Intraoperative Detection of Human Glioma

Zhang

Zhou

et al. 2023

Cancers

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There is still a lack of reliable intraoperative tools for glioma diagnosis and to guide the maximal safe resection of glioma. We report continuing work on the optical biopsy method to detect glioma grades and assess glioma boundaries intraoperatively using the VRR-LRRTM Raman analyzer, which is based on the visible resonance Raman spectroscopy (VRR) technique. A total of 2220 VRR spectra were collected during surgeries from 63 unprocessed fresh glioma tissues using the VRR-LRRTM Raman analyzer. After the VRR spectral analysis, we found differences in the native molecules in the fingerprint region and in the high-wavenumber region, and differences between normal (control) and different grades of glioma tissues. A principal component analysis–support vector machine (PCA-SVM) machine learning method was used to distinguish glioma tissues from normal tissues and different glioma grades. The accuracy in identifying glioma from normal tissue was over 80%, compared with the gold standard of histopathology reports of glioma. The VRR-LRRTM Raman analyzer may be a new label-free, real-time optical molecular pathology tool aiding in the intraoperative detection of glioma and identification of tumor boundaries, thus helping to guide maximal safe glioma removal and adjacent healthy tissue preservation.

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“…RS has been used to study nerve myelin during excitation or the effect of neurotransmitter on nerve fibre. It has been shown that the changes in the C-C bonds of the fatty acids can be detected as well as the changes in the conformation [78]. This knowledge will improve our understanding of the mechanisms of lipid-lipid interactions in myelin and many processes associated with various diseases, such as multiple sclerosis, trauma and AD.…”

Section: Determination Of Metabolitesmentioning

confidence: 94%

Raman Spectroscopy and Its Modifications Applied to Biological and Medical Research

Allakhverdiev

Khabatova

Kossalbayev

et al. 2022

Cells

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Nowadays, there is an interest in biomedical and nanobiotechnological studies, such as studies on carotenoids as antioxidants and studies on molecular markers for cardiovascular, endocrine, and oncological diseases. Moreover, interest in industrial production of microalgal biomass for biofuels and bioproducts has stimulated studies on microalgal physiology and mechanisms of synthesis and accumulation of valuable biomolecules in algal cells. Biomolecules such as neutral lipids and carotenoids are being actively explored by the biotechnology community. Raman spectroscopy (RS) has become an important tool for researchers to understand biological processes at the cellular level in medicine and biotechnology. This review provides a brief analysis of existing studies on the application of RS for investigation of biological, medical, analytical, photosynthetic, and algal research, particularly to understand how the technique can be used for lipids, carotenoids, and cellular research. First, the review article shows the main applications of the modified Raman spectroscopy in medicine and biotechnology. Research works in the field of medicine and biotechnology are analysed in terms of showing the common connections of some studies as caretenoids and lipids. Second, this article summarises some of the recent advances in Raman microspectroscopy applications in areas related to microalgal detection. Strategies based on Raman spectroscopy provide potential for biochemical-composition analysis and imaging of living microalgal cells, in situ and in vivo. Finally, current approaches used in the papers presented show the advantages, perspectives, and other essential specifics of the method applied to plants and other species/objects.

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Study of myelin structure changes during the nerve fibers demyelination

Cited by 8 publications

References 23 publications

Demyelinating polyneuropathy in goats lacking prion protein

Demyelinating polyneuropathy in goats lacking prion protein

A Handheld Visible Resonance Raman Analyzer Used in Intraoperative Detection of Human Glioma

Raman Spectroscopy and Its Modifications Applied to Biological and Medical Research

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