Study of the interaction of β-cyclodextrin with phospholipid monolayers by surface pressure measurements and fluorescence microscopy

X-ray grazing incidence diffraction and Langmuir monolayer studies of the interaction of β-cyclodextrin with model lipid membranes

“…Moreover, sterol desorption from cholesterol/phospholipid mixed systems was found to be notably retarded, even though the removal of phospholipid molecules was not observed [23][24][25][26][27][28][29][30]. On the contrary, phospholipids' desorption from monolayers has recently been described by other authors [31]. For instance, methylated bcyclodextrin was applied by Giocondi et al to manipulate the composition of model lipid rafts and one of the important conclusions was that cyclodextrins were capable of sphingomyelin removal from PC/SM supported bilayers [32].…”

Computational microscopy of cyclodextrin mediated cholesterol extraction from lipid model membranes

“…Thus, DRMs solubilization after cyclodextrin treatment was indicative of it being part of a raft component. In addition, if a biological process in living cells was disrupted by cyclodextrin treatment, the process was considered to be raft-based3456789. From the chemical point of view, cyclodextrins (CD) are composed of several glucose units, linked together by α -1,4-glucosidic bonds.…”

“…To characterize the ability of β -CD to uptake cholesterol from biomembranes, many studies have been devoted to model membranes689111213141516. Ohvo and Slotte12 demonstrated that the desorption rates of various sterols from the surface film to β -CD solution depend on the relative polarity of sterols and increase considerably with increasing surface pressure.…”

Interaction of fluorescent phospholipids with cyclodextrins

“…Several of those studies used cyclodextrins (CDs) e.g., for triggering lipid extraction from the membrane (Tanhuanpää and Somerharju, 1999;Grauby-Heywang and Turlet, 2008;Pillai et al, 2009;Huang and London, 2013). CDs represent a family of cyclic oligosaccharides consisting of a-(1-4)-linked D-glycopyranose units that are primarily formed by the enzymatic degradation of starch (Del Valle, 2004).…”