Interaction of fluorescent phospholipids with cyclodextrins

Denz, Manuela; Haralampiev, Ivan; Schiller, S.; Szente, Lajos; Herrmann, Andreas; Huster, Daniel; Müller, Peter

doi:10.1016/j.chemphyslip.2015.07.017

Cited by 12 publications

(19 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fluorescent analogs of PLs and Chol have been widely used to investigate the structural and dynamic properties of membranes. The interaction of CDs with fluorescent-labeled Chol (Milles et al, 2013) and PLs (Denz et al, 2016) bearing 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD)…”

Section: Extraction Of Lipid Membrane Components From Biomimetic and Biological Membranesmentioning

confidence: 99%

“…Excitation of the BODIPY or NBD moieties induced a large FRET signal; large rhodamine fluorescence was obtained when the fluorophores came close together within the membrane. Consequently, the CD-mediated extraction of the fluorescent analogs reduced their concentration in the membrane, resulting in a decrease of FRET signals (Denz et al, 2016).…”

Section: Extraction Of Lipid Membrane Components From Biomimetic and Biological Membranesmentioning

confidence: 99%

“…Moreover, Kilsdonk et al (1995) demonstrated that HP-β-CD-induced a rapid Chol release from mouse L-cell fibroblasts over an initial period (up to about 2 h) until the equilibrium between Chol in the outer medium and in the membrane compartment was reached. Also, the kinetics of the PL extraction from liposomes by Me-α-CD and Me-β-CD was fitted to a biexponential equation (Denz et al, 2016).…”

Section: Cross 1999mentioning

confidence: 99%

“…The interaction of methylated CDs such as Me-α-CD, Me-β-CD, and Me-γ-CD, with liposome membrane containing NBD-labeled PLs was examined by detecting FRET between the NBD and Rh-PE as described earlier (Denz et al, 2016). Me-α-CD and Me-ß-CD were similarly efficient in inducing a high efflux of fluorescent-labeled PLs embedded in the membrane,…”

Section: Cyclodextrin Type and Concentrationmentioning

confidence: 99%

See 3 more Smart Citations

Cyclodextrin-membrane interaction in drug delivery and membrane structure maintenance

Hammoud

Khreich

Auezova

et al. 2019

International Journal of Pharmaceutics

View full text Add to dashboard Cite

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

show abstract

Section: Extraction Of Lipid Membrane Components From Biomimetic and Biological Membranesmentioning

confidence: 99%

Section: Extraction Of Lipid Membrane Components From Biomimetic and Biological Membranesmentioning

confidence: 99%

Section: Cross 1999mentioning

confidence: 99%

Section: Cyclodextrin Type and Concentrationmentioning

confidence: 99%

See 2 more Smart Citations

Cyclodextrin-membrane interaction in drug delivery and membrane structure maintenance

Hammoud

Khreich

Auezova

et al. 2019

International Journal of Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…The hydrophobic cavity of CDs can also form complexes with fatty acids and phospholipids (Szente and Fenyvesi, 2017). CDs have thus been used to remove lipids from native (Vahedi et al, 2020, Startek et al, 2019) and model membranes (Sanchez et al, 2011, Denz et al, 2016), a process that has already been linked to increases in membrane tension (Biswas et al, 2019). CDs also exhibit differential lipid selectivity.…”

Section: Introductionmentioning

confidence: 99%

Cyclodextrins increase membrane tension and are universal activators of mechanosensitive channels

Cox

Zhang

Zhou

et al. 2021

Preprint

View full text Add to dashboard Cite

The bacterial mechanosensitive channel of small conductance, MscS, has been extensively studied to understand how mechanical forces are converted into the conformational changes that underlie mechanosensitive (MS) channel gating. We showed that lipid removal by β-cyclodextrin can mimic membrane tension. Here, we show that all cyclodextrins (CDs) can activate reconstituted E. coli MscS, that MscS activation by CDs depends on CD-mediated lipid removal, and that the CD amount required to gate MscS scales with the channels sensitivity to membrane tension. CD-mediated lipid removal ultimately causes MscS desensitization, which we show is affected by the lipid environment. CDs can also activate the structurally unrelated MscL. While many MS channels respond to membrane forces, generalized by the force-from-lipids principle, their different molecular architectures suggest that they use unique ways to convert mechanical forces into conformational changes. CDs emerge as a universal tool for the structural and functional characterization of unrelated MS channels.

show abstract

Cyclodextrins for Probing Plasma Membrane Lipids

Vahedi

Farnoud

2020

Springer Protocols Handbooks

View full text Add to dashboard Cite

Interaction of fluorescent phospholipids with cyclodextrins

Cited by 12 publications

References 70 publications

Cyclodextrin-membrane interaction in drug delivery and membrane structure maintenance

Cyclodextrin-membrane interaction in drug delivery and membrane structure maintenance

Cyclodextrins increase membrane tension and are universal activators of mechanosensitive channels

Cyclodextrins for Probing Plasma Membrane Lipids

Contact Info

Product

Resources

About