Study of the inhibitory effect of the product dihydroxyacetone on Gluconobacter oxydans in a semi-continuous two-stage repeated-fed-batch process

Abstract

The influence of the product inhibition by dihydroxyacetone (DHA) on Gluconobacter oxydans for a novel semi-continuous two-stage repeated-fed-batch process was examined quantitatively. It was shown that the culture was able to grow up to a DHA concentration of 80 kg m(-3) without any influence of product inhibition. The regeneration capability of the reversibly product inhibited culture from a laboratory-scale bioreactor system was observed up to a DHA concentration of about 160 kg m(-3). At higher DHA concentrations, the culture was irreversibly product inhibited. However, due to the robust membrane-bound glycerol dehydrogenase of G. oxydans, product formation was still active for a prolonged period of time. The reachable maximum final DHA concentration was as high as 220 kg m(-3). The lag phases for growth increased exponentially with increasing DHA threshold values of the first reactor stage. These results correlated well with fluorescence in situ hybridization (FISH) measurements confirming that the number of active cells decreased exponentially with increasing DHA concentrations.

Effect of glycerol and dihydroxyacetone concentrations in the culture medium on the growth of acetic acid bacteria Gluconobacter oxydans ATCC 621

“…This concentration of the product in the reaction medium induced irreversible destruction of cells of acetic acid bacteria. This could be due to the inhibiting effect of DHa on the activity of enzymes of the pentose cycle [4,6,25].…”

Small substrate, big surprise: fold, function and phylogeny of dihydroxyacetone kinases

“…The oxidation reaction takes place on the outer face of the membrane, and Dha is released into the medium [14]. Industrial Gluconobacter strains yield up to 220 kg/m 3 of Dha because the dehydrogenase retains activity even after irreversible growth inhibition of cells by Dha [15]. (iii) Methylotrophic yeasts produce Dha during methanol assimilation.…”

Oxidation of Reactive Alcohols with Hydrogen Peroxide Catalyzed by Manganese Complexes

“…Gold catalysts are especially active in the glycerol oxidation [48][49][50][51][52][53][54]. Some publications have been devoted to the aerobic oxidation of glycerol catalyzed by enzymes and living organisms [45][46][47][48][49][50][51][52][53][54][55][56][57][58], particularly dihydroxyacetone is produced via the oxidation of glycerol with the participation of bacterium Gluconobacter oxydans [59]. Only restricted number of papers reported metal-catalyzed heterogeneous glycerol oxidations with H 2 O 2 [60][61][62].…”