Study of the incompatibility and replication of the 70-kb virulence plasmid of Yersinia

“…The pYV plasmids are incompatible with plasmid F as a result of the presence of an incD incompatibility determinant (2). This incD locus is part of the partition system of plasmid F (33).…”

mentioning

confidence: 99%

Structural and functional similarities between the replication region of the Yersinia virulence plasmid and the RepFIIA replicons

Vanooteghem

Cornelis

1990

J Bacteriol

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“…The transferability of ESBL genes was assessed by mating with E. coli J53rif (12). When mating was negative, transformation into E. coli TOP10 (Invitrogen, Saint-Aubin, France) was attempted by electroporation of whole-plasmid DNA, as described previously (6).…”

Section: Subjectsmentioning

confidence: 99%

Characterization of Fecal Extended-Spectrum-β-Lactamase-Producing Escherichia coli in a Remote Community during a Long Time Period

Woerther

Angebault

Jacquier

et al. 2013

Antimicrob Agents Chemother

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“…Mating experiments were performed as previously described [9] with E. coli C600 (rifampicin resistant) as a recipient. Selective agents were used at the following concentrations: 200 mg/L for rifampicin and 8 mg/L for cefotaxime.…”

Section: Conjugation Experimentsmentioning

confidence: 99%

382 CTX-M-15 extended-spectrum β-lactamases in Enterobacteriaceae in the intensive care unit of Tlemcen Hospital, Algeria

Ahmed¹,

Ayad²,

Mesli³

et al. 2012

East Mediterr Health J

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The aim of this study was to detect extended-spectrum β-lactamases (ESBL) in Enterobacteriaceae isolates in the intensive care unit (ICU) of Tlemcen hospital in north-western Algeria. Antimicrobial susceptibility testing, molecular typing, characterization of ESBL-encoding genes and the genetic environment, conjugation experiments and plasmid analysis were carried out. In all, 28 Enterobacteriaceae isolates were isolated from specimens recovered from patients in the ICU and 2 from surfaces of the unit. Of these, 11 isolates (4 Escherichia coli, 5 Klebsiella pneumoniae and 2 Enterobacter cloacae) produced ESBL of the CT-X-M-15 type. Molecular typing of the isolates showed the clonal nature of 4 K. pneumoniae isolates. The bla CTXM-15 gene was genetically linked to insertion sequence ISEcp1B and was transferable by conjugation from 3 isolates. Regular monitoring of resistance mechanisms, the establishment of a prevention strategy, and more rational and appropriate use of antibiotics are needed.

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Study of the incompatibility and replication of the 70-kb virulence plasmid of Yersinia

Cited by 30 publications

References 32 publications

Structural and functional similarities between the replication region of the Yersinia virulence plasmid and the RepFIIA replicons

Structural and functional similarities between the replication region of the Yersinia virulence plasmid and the RepFIIA replicons

Characterization of Fecal Extended-Spectrum-β-Lactamase-Producing Escherichia coli in a Remote Community during a Long Time Period

382 CTX-M-15 extended-spectrum β-lactamases in Enterobacteriaceae in the intensive care unit of Tlemcen Hospital, Algeria

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