Study of the epidermis ablation effect on the efficiency of optical clearing of skin in vivo

Genina, Elina A.; Ksenofontova, N. S.; Bashkatov, Alexey N.; Terentyuk, Georgy S.; Tuchin, Valery V.

doi:10.1070/qel16378

Cited by 9 publications

(4 citation statements)

References 7 publications

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“…As the skin absorption coefficient μ a is far less that the scattering coefficient μ s [1], the attenuation coefficient μ t may be considered approximately equal to the scattering coefficient, so R(z) may be approximated by the expression [33].…”

Section: Methodsmentioning

confidence: 99%

R (z) = A \exp (- μ_{s} z) + B,

where A is the proportionality factor equal to P 0 ⋅a ( z ), P 0 is the optical power of the beam falling on the tissue surface, a ( z ) is determined by the tissue local ability to scatter light backwards, which depends on a local variation of the refractive index, and B is the background signal.…”

Section: Methodsmentioning

confidence: 99%

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Optical clearing of human skin: Molecular modeling and in vivo OCT study

Berezin,

Grabarchuk,

Lichter

et al. 2023

Journal of Biophotonics

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The results of in vivo immersion optical clearing of human skin under the action of two different optical clearing agents (OCAs), such as an aqueous sucrose solution and a radiographic contrast agent Omnipaque™ 300 (iohexol), were obtained with the use of optical coherence tomography (OCT) method. The rate of reduction of light scattering coefficient, obtained through an averaged A‐scan of the OCT image in the region of dermis within the depths from 350 to 700 μm, were determined to evaluate the efficiency of optical clearing (EOC). The correlations between the EOC and the energy of intermolecular interaction of OCAs with a fragment of collagen peptide have been established as a result of molecular modeling by quantum chemistry methods HF/STO3G/DFT/B3LYP/6—311G(d) of a number of OCAs (glycerol, iohexol, sucrose, ribose, fructose, glucose) with mimetic peptide of collagen (GPH)3.

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Section: Methodsmentioning

confidence: 99%

R (z) = A \exp (- μ_{s} z) + B,

Section: Methodsmentioning

confidence: 99%

Optical clearing of human skin: Molecular modeling and in vivo OCT study

Berezin,

Grabarchuk,

Lichter

et al. 2023

Journal of Biophotonics

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“…For evaluation of µ t value as an exponential parameter, that shows the best correlation with the curve, the nonlinear least-squares method was used [35,36]. Values of µ t were defined for each animal, were averaged by groups and normalized by initial value.…”

Section: Methodsmentioning

confidence: 99%

Influence of immersion agents on optical parameters of bio-tissues during laser photothermal therapy of tumor: pilot study

Genin

A.B.

N.A.

et al. 2022

Optics and Spectroscopy

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Combined use of an immersion agent with low-intensity laser irradiation for optical clearing of skin before plasmon photothermal therapy (PPTT) procedure is offered. Pilot study results of influence of the immersion agents on optical parameters of skin, subdermal connective tissue and model tumor of rats in vivo at hyperthermia during PPTT are presented. Model of alveolar liver cancer --- cholangiocarcinoma, transplanted under the skin, was used as a model tumor. For PPTT the gold nanorods with absorption band in the area of diode laser radiation (808 nm) were introduced. Monitoring of light attenuation coefficient change in skin at optical clearing was performed using optical coherent tomography. Measurements of optical parameters of the complete tumor and its layers were performed using spectrometers in a wave length range of 350-2200 nm. Reduction of skin thermal damage during PPTT with preliminary optical clearing using immersion agent (mixture of 70% glycerol water solution and 10% DMSO) and low-intensity laser irradiation at wave length of 808 nm is observed. Keywords: gold nanorods, IR laser radiation, optical clearing, optical parameters, plasmon photothermal therapy.

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“…It should also take into account the physiological response of living tissue to the action of the agent and its redistribution with a possible rapid exit from the area of interest. Therefore, the use of laser ablation and microporation in the stratum corneum ex vivo showed an increase in the penetration of OCA into the dermis; however, these treatments caused skin erythema and edema when applied in vivo, which increased both the absorption and scattering of light [ 40 , 50 ]. On the contrary, OCA in combination with sonophoresis caused rapid and safe optical clearing of the skin [ 41 ].…”

mentioning

confidence: 99%