Tissue Optical Clearing: State of the Art and Prospects

Genina, Elina A.

doi:10.3390/diagnostics12071534

Cited by 4 publications

(3 citation statements)

References 51 publications

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“…61 However, the depth of optical detection of skin can be increased with the usage of the tissue optical clearing technique, which is based on altering the optical properties of tissue through the application of immersion optical cleaning agents to it. [94][95][96] Besides contributing to the image contrast improvement, OCAs can play the role of chemical enhancers for particle penetration through and into the skin. Recently, we have demonstrated the possibility to deliver and detect vaterite particles in hair follicles exploiting optical clearing for OCT. 61 According to the obtained results, the mixture of oleic acid and PEG-400 (20 : 80 v/v) utilized as a solvent to suspend the carriers prior to topical application has demonstrated sufficient improvement of their penetration and detection depth.…”

Section: Intrafollicular Delivery and Degradation Of The Cp-caco 3 Ca...mentioning

confidence: 99%

Biodegradable calcium carbonate carriers for the topical delivery of clobetasol propionate

Saveleva,

Verkhovskii,

Demina

et al. 2024

J. Mater. Chem. B

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Section: Intrafollicular Delivery and Degradation Of The Cp-caco 3 Ca...mentioning

confidence: 99%

Biodegradable calcium carbonate carriers for the topical delivery of clobetasol propionate

Saveleva,

Verkhovskii,

Demina

et al. 2024

J. Mater. Chem. B

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“…This is due to light scattering caused by refractive index (RI) mismatches at the interfaces between biological tissue components such as lipids, proteins, and water [55]. Optical clearing agents may be key in solving this problem in the future [56]. We believe that this approach will significantly expand the range of applications of the developed photoconvertible labels.…”

Section: Hmscs Trackingmentioning

confidence: 99%

Labeling and Tracking of Individual Human Mesenchymal Stromal Cells Using Photoconvertible Fluorescent Microcapsules

Sindeeva,

Demina,

Kozyreva

et al. 2023

IJMS

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The behavior and migration of human mesenchymal stromal cells (hMSCs) are focal points of research in the biomedical field. One of the major aspects is potential therapy using hMCS, but at present, the safety of their use is still controversial owing to limited data on changes that occur with hMSCs in the long term. Fluorescent photoconvertible proteins are intensively used today as “gold standard” to mark the individual cells and study single-cell interactions, migration processes, and the formation of pure lines. A crucial disadvantage of this method is the need for genetic modification of the primary culture, which casts doubt on the possibility of exploring the resulting clones in personalized medicine. Here we present a new approach for labeling and tracking hMSCs without genetic modification based on the application of cell-internalizable photoconvertible polyelectrolyte microcapsules (size: 2.6 ± 0.5 μm). These capsules were loaded with rhodamine B, and after thermal treatment, exhibited fluorescent photoconversion properties. Photoconvertible capsules demonstrated low cytotoxicity, did not affect the immunophenotype of the hMSCs, and maintained a high level of fluorescent signal for at least seven days. The developed approach was tested for cell tracking for four days and made it possible to trace the destiny of daughter cells without the need for additional labeling.

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“…The mean alveolar number in humans is 274-790 million with the mean size of a single alveolus of 4.2 × 10 6 μm 3 or approximately 200 μm in diameter (Ochs et al 2004). Air-filled lungs present significant challenges for optical imaging including optical coherence tomography (OCT) (Quirk et al 2014;Ding et al 2021), confocal and two-photon microscopy, and Raman spectroscopy, because of the large refractive-index mismatch between alveoli walls and the enclosed air-filled region, major challenges are associated with the improvement of imaging resolution even further to the subcellular level (Yu et al 2021;Lin et al 2020;Yanina et al 2020;Jaafar et al 2021;Genina 2022).…”

Section: Introductionmentioning

confidence: 99%

Optical clearing and testing of lung tissue using inhalation aerosols: prospects for monitoring the action of viral infections

et al. 2022

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Optical clearing of the lung tissue aims to make it more transparent to light by minimizing light scattering, thus allowing reconstruction of the three-dimensional structure of the tissue with a much better resolution. This is of great importance for monitoring of viral infection impact on the alveolar structure of the tissue and oxygen transport. Optical clearing agents (OCAs) can provide not only lesser light scattering of tissue components but also may influence the molecular transport function of the alveolar membrane. Air-filled lungs present significant challenges for optical imaging including optical coherence tomography (OCT), confocal and two-photon microscopy, and Raman spectroscopy, because of the large refractive-index mismatch between alveoli walls and the enclosed air-filled region. During OCT imaging, the light is strongly backscattered at each air-tissue interface, such that image reconstruction is typically limited to a single alveolus. At the same time, the filling of these cavities with an OCA, to which water (physiological solution) can also be attributed since its refractive index is much higher than that of air will lead to much better tissue optical transmittance. This review presents general principles and advances in the field of tissue optical clearing (TOC) technology, OCA delivery mechanisms in lung tissue, studies of the impact of microbial and viral infections on tissue response, and antimicrobial and antiviral photodynamic therapies using methylene blue (MB) and indocyanine green (ICG) dyes as photosensitizers.

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Tissue Optical Clearing: State of the Art and Prospects

Abstract: The term “tissue optical clearing” (TOC) came into use at the end of the 20th century and is associated with the development of methods for controlling tissue scattering properties using the refractive index matching effect [...]

Cited by 4 publications

References 51 publications

Biodegradable calcium carbonate carriers for the topical delivery of clobetasol propionate

Biodegradable calcium carbonate carriers for the topical delivery of clobetasol propionate

Labeling and Tracking of Individual Human Mesenchymal Stromal Cells Using Photoconvertible Fluorescent Microcapsules

Optical clearing and testing of lung tissue using inhalation aerosols: prospects for monitoring the action of viral infections

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