Study of the efficacy of 5 ALA-mediated photodynamic therapy on human larynx squamous cell carcinoma (Hep2c) cell line

Khursid, A.; Firdous, S.; Zaidi, S. S. Z.; Salman, R.; Ikram, Manzoor

doi:10.1134/s1054660x10130086

Cited by 32 publications

(26 citation statements)

References 28 publications

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“…Higher absorption imply in higher production of singlet oxygen and therefore higher photodynamic action and better PDT outcome. This study is in agreement with earlier studies using a variety of photosensitizers in different chemical and biological environments [38][39][40][41][42].…”

Section: Discussionsupporting

confidence: 93%

Analysis of the combined effect of lasers of different wavelengths for PDT outcome using 600, 630, and 660 nm

et al. 2011

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Abstract:We investigated the effects of photodynamic therapy (PDT) outcome when combining three laser systems that produce light in three different wavelengths (600, 630, and 660 nm). Cooperative as well as independent effects can be observed. We compared the results of the combined wavelengths of light with the effect of single laser for the excitation of the photosensitizer. In the current experiment, the used photosensitizer was Photogem R (1.5 mg/kg). Combining two wavelengths for PDT, their cumulative dose and different penetrability may change the overall effect of the fluence of light, which can be effective for increasing the depth of necrosis. This evaluation was performed by comparing the depth and specific aspect of necrosis obtained by using single and dual wavelengths for irradiation of healthy liver of male Wistar rats. We used 15 animals and divided them in five groups of three animals. First, Photogem R was administered; follow by measurement of the fluorescence spectrum of the liver before PDT to confirm the level of accumulation of photosensitizer in the tissue. After that, an area of 1 cm 2 of the liver was illuminated using different laser combinations. Qualitative analysis of the necrosis was carried out through histological and morphological study.

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Section: Discussionsupporting

confidence: 93%

Analysis of the combined effect of lasers of different wavelengths for PDT outcome using 600, 630, and 660 nm

et al. 2011

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“…The negligible differences in results for two concentrations might correspond to the cellular saturation of ALA absorption for a particular concentration. A. Khursid et al [13] ALA incubation time, hours 0 μg/ml 20 μg/ml 40 μg/ml 50 μg/ml Figure 1 (online color at www.lphys.org) ALA absorbance versus ALA incubation time. Each data point corresponds to mean ±σ (n = 7) the ALA absorbance observation it was explored that the cellular uptake of ALA is very low in first 22 hours of time of incubation.…”

Section: Resultsmentioning

confidence: 99%

“…The cells were exposed to light through the clear bottom of the plate using a semiconductor diode laser (LPhT-630/675-01-BIOSPEC, Russia) at a wavelength of 635 nm [13]. Laser beams were transmitted to the cells in a 6-mm diameter well (area of 0.294 cm 2 ) by an optical fiber (BIOSPEC, TF-D, Russia).…”

Section: Light Exposurementioning

confidence: 99%

Study of the efficacy of 5-ALA mediated photodynamic therapy on human rhabdomyosarcoma cell line (RD)

Fakhar-e-Alam¹,

Firdous²,

Zaidi³

et al. 2010

Laser Phys. Lett.

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The aim of this study was to investigate the mechanism of cell death by photodynamic therapy (PDT) in the Rhabdomyosarcoma (RD) cell line. The present study evaluates the effects of photodynamic therapy (PDT) with 5-ALA as photosensitizer using human muscle cancer cells as experimental model. We study the photosensitizer uptake, cytotoxicity, phototoxicity, and cellular viability of the RD cells which was estimated by means of neutral-red spectrophotometric assay. The given experiment was consisted of two steps. For the first one, RD cells were exposed to 5-ALA at concentrations of 0 up to 1000 μg of ALA/ml in minimum essential medium (MEM). The optimal uptake of photosensitizer (5-ALA) in RD cells was investigated by means of spectrometric measurements. Cells viability was determined by means of neutral red assay (NRA). In the second step, 5-ALA exposed RD cells were irradiated with red light (a diode laser, λ = 635 nm) at total light dose of 80 J/cm 2 . The influence of different incubation times and concentrations of 5-ALA, different irradiation doses and various combinations of photosensitizer and light doses on the viability of RD cells were investigated. It was observed that sensitizer concentration or light doses have no significant effect on cells viability when studied independently. The maximal cellular uptake occurred after 47 hours in vitro incubation. The phototoxic assay showed that ALA-PDT induced killing of 76% of the cells at 250 μg/ml drug dose and 80 J/cm 2 light dose.

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“…Microscopic images of PDT treated normal liver show a sharp edge between the necrotic region and the nonnecrotic region [13,14]. This observation associated with a model of light-tissue interaction shows that cell death depends exclusively on the amount of cell toxicity, generated by the local photoproducts.…”

Section: Introductionmentioning

confidence: 91%

Experimental evidence and model explanation for cell population characteristics modification when applying sequential photodynamic therapy

et al. 2011

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We present experimental evidence of the existence of cell variability in terms of threshold light dose for Hep G2 (liver cancer cells) cultured. Using a theoretical model to describe the effects caused by successive photodynamic therapy (PDT) sessions, and based on the consequences of a partial response we introduce the threshold dose distribution concept within a tumor. The experimental model consists in a stack of flasks, and simulates subsequent layers of a tissue exposed to PDT application. The result indicates that cells from the same culture could respond in different ways to similar PDT induceddamages. Moreover, the consequence is a partial killing of the cells submitted to PDT, and the death fraction decreased at each in vitro PDT session. To demonstrate the occurrence of cell population modification as a response to PDT, we constructed a simple theoretical model and assumed that the threshold dose distribution for a cell population of a tumor is represented by a modified Gaussian distribution.Microscopic image (100×) of a necrosis caused by PDT in a rat liver. The edge is the depth of necrosis

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Study of the efficacy of 5 ALA-mediated photodynamic therapy on human larynx squamous cell carcinoma (Hep2c) cell line

Cited by 32 publications

References 28 publications

Analysis of the combined effect of lasers of different wavelengths for PDT outcome using 600, 630, and 660 nm

Analysis of the combined effect of lasers of different wavelengths for PDT outcome using 600, 630, and 660 nm

Study of the efficacy of 5-ALA mediated photodynamic therapy on human rhabdomyosarcoma cell line (RD)

Experimental evidence and model explanation for cell population characteristics modification when applying sequential photodynamic therapy

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