Study of the effects of nesfatin-1 on gastric function in obese rats

Abstract

AIMTo investigate the effects of nesfatin-1 on gastric function in obese rats.METHODSThe obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats were determined after treatment with different drug concentrations of nesfatin-1 and administration routes. Based on this, the expression of H+/K+-ATPase was measured using RT-PCR and western blot to preliminarily explore the mechanism of gastric acid secretion changes.RESULTSBody weight, body length, and Lee’s index of the rats significantly increased in the high-fat diet-induced obese rat model. Two hours after lateral intracerebroventricular injection of nesfatin-1, the gastric emptying rate and gastric acid secretory capacity of rats decreased. Four hours after injection, both were restored to normal levels. In addition, the expression of H+/K+-ATPase decreased and moved in line with changes in gastric acid secretory capacity. This in vivo experiment revealed that intracerebroventricular injection of nesfatin-1, rather than intravenous injection, could suppress gastric function in obese rats. Moreover, its effect on the gastric emptying and gastric acid secretory capacity of rats is dose-dependent within a certain period of time.CONCLUSIONThrough this research, we provide a theoretical basis for further studies on nesfatin-1, a potential anti-obesity drug.

Novel transmitters in brain stem vagal neurocircuitry: new players on the pitch

“…Central (4 th -ventricular) administration of nesfatin-1 increases c-Fos expression in the rat DMV and induces a dose-dependent increase in calcium influx in the overwhelming majority of cultured DMV neurons, suggesting a direct regulatory action on vagal outflow (96). Indeed, central (icv) administration of nesfatin-1 inhibited the antroduodenal motility of mice in a dose-dependent fashion (2) and reduced gastric emptying of rats (88,100). In addition to its actions to modulate gastric motor functions, nesfatin-1 has also been shown to inhibit secretory functions, including both basal and vagally stimulated (2-deoxy-D-glucose-induced) gastric acid production (96,100).…”

“…Indeed, central (icv) administration of nesfatin-1 inhibited the antroduodenal motility of mice in a dose-dependent fashion (2) and reduced gastric emptying of rats (88,100). In addition to its actions to modulate gastric motor functions, nesfatin-1 has also been shown to inhibit secretory functions, including both basal and vagally stimulated (2-deoxy-D-glucose-induced) gastric acid production (96,100).…”

The Effects of Feeding Obese Rats by Bee Bread on IL-6 Expression in Rat Stomach

“…Obesity is a global public health priority. With the improvement of people's standard of living, the number of obese Chinese has grown to an annual rate of 38.1% over the past several years [1]. Obesity is currently one of the major health concerns in developed countries.…”

Ghrelin and NUCB2/Nesfatin‐1 Co‐Localization With Digestive Enzymes in the Intestine of Pejerrey (Odontesthes bonariensis)

“…While ghrelin seems to be an important modulator of gastrointestinal functions in mammals, whether this function is conserved in fish is poorly understood . Some studies have demonstrated that ghrelin contributes to gastric emptying and gastric acid secretory capacity in rats (Tian et al, 2014;Yang et al, 2017). To date, no data is available on gastric functions of nesfatin-1 in fish.…”