Study of the cytotoxic and irritant effects of skin cleansing soaps

Abstract

Introducción: El jabón para el aseo cutáneo es de empleo común entre la población, sin embargo, es posible que cause daño a las células de la piel y modifique la barrera cutánea. Objetivo: Determinar el efecto citotóxico de los jabones en queratinocitos cultivados in vitro y correlacionarlo con la irritación clínica. Método: Se realizó una encuesta para conocer los jabones comerciales más utilizados y su cantidad; posteriormente, se evaluó su citotoxicidad en cultivos de queratinocitos humanos mediante el método de resazurina. Los jabones con mayor y menor citotoxicidad se aplicaron en piel de voluntarios sanos para evaluar su efecto en la barrera cutánea mediante ensayos de colorimetría y pérdida transepidérmica de agua. Resultados: De los jabones analizados, 37 % demostró ser tóxico para los queratinocitos in vitro. El jabón con mayor toxicidad indujo el mayor índice de eritema y pérdida transepidérmica de agua, en comparación con el jabón menos tóxico y el vehículo empleado como solución control. Conclusión: Los jabones comercializados para el aseo cutáneo pueden incluir ingredientes químicos que dañan los queratinocitos humanos y causan irritación subclínica de la barrera cutánea. Su utilización puede agravar dermatosis preexistentes, generar dermatitis xerósica o de contacto irritativa y causar atrofia y dermatoporosis.

Effect of New Surfactants on Biological Properties of Liquid Soaps

“…48 h treatment of HaCat cells of LS I caused significant cytotoxicity in a range above 0.5%. This is in line with previous research in which a high concentration of soaps or other care products significantly reduces cell number after a short [ 36 , 37 ] and long exposition [ 38 ]. Both Cocamidopropyl Betaine (LS II) and CocamidopropylHydroxysultaine (LS III) did not affect the cytotoxic properties of LS I.…”

The effect of electrical stimulation on skin vulnerability to irritants

“…We used TWEL and visual erythema scores to assess skin irritation, as they have been extensively utilized in several studies for this purpose. [19][20][21][22][23][24] This study demonstrated that ES makes the skin more sensitive to irritating stimuli such as SLS, even at concentrations as low as 0.5%. Even more so, with higher concentrations of SLS (5%) com-TA B L E 1 Transepidermal water loss (TWEL) mean values after 24 h of patch removal for areas 1, 2, 3, and 4 on the experimental and control forearms.…”

“…We used TWEL and visual erythema scores to assess skin irritation, as they have been extensively utilized in several studies for this purpose 19–24 . This study demonstrated that ES makes the skin more sensitive to irritating stimuli such as SLS, even at concentrations as low as 0.5%.…”

“…Moreover, research suggests that ES can improve the absorption and efficacy of skincare products by augmenting the skin's absorption of topically applied substances 18 . Since ES can alter the skin's properties and increase its permeability, it is crucial to consider whether this immediate effect will make the skin area between the electrodes more sensitive to external irritants or chemicals 19 …”

“…18 Since ES can alter the skin's properties and increase its permeability, it is crucial to consider whether this immediate effect will make the skin area between the electrodes more sensitive to external irritants or chemicals. 19 The chemical compound sodium lauryl sulfate (SLS) is recognized as the standard stimulant. This substance was used to evaluate the skin irritation to exogenous stimulants.…”