Study of Survivin and X-Linked Inhibitor of Apoptosis Protein (XIAP) Genes in Acute Myeloid Leukemia (AML)

Abstract

Apoptosis deregulation is important for cancer development, chemotherapy response, and prognosis. Survivin and X-linked inhibitor of apoptosis protein (XIAP) are 2 members of the inhibitor of apoptosis proteins family (IAP). We used semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to determine the levels of expression of survivin and XIAP in 30 patients with de novo acute myeloid leukemia (AML) and 20 age- and sex-matched healthy volunteers. Survivin and XIAP overexpression were detected in 36.7% and 43.3% of cases, respectively. Patients with overexpression of either survivin or XIAP showed unfavorable response to chemotherapy in 81.2% and 91.7%, respectively. Also, these cases showed shorter median survival time (30 days) compared to patients with normal expression of either survivin or XIAP (150 days and 180 days). Patients with overexpression of both survivin and XIAP showed unfavorable response to induction therapy in 100% of the patients and the shortest median survival (30 days). These findings suggest that survivin and XIAP may have a role in leukemogenesis and provide prognostic information.

Exogenous Morphine Inhibits Human Gastric Cancer MGC-803 Cell Growth by Cell Cycle Arrest and Apoptosis Induction

“…Survivin expression is an unfavorable prognostic factor in several malignancies, such as gastric carcinoma, breast carcinoma and acute myeloid leukemia. The higher the survivin expression, the worse the prognosis (Ibrahim et al, 2012). Clearly, inhibition of survivin activity would facilitate propagation of caspase cascade and enhance apoptosis (Chen et al, 2012;Dai et al, 2012).…”

Apoptosis of HL-60 human leukemia cells induced by Asiatic acid through modulation of B-cell lymphoma 2 family proteins and the mitogen-activated protein kinase signaling pathway

“…Survivin (BIRC5) is a member of the family of inhibitors of apoptosis proteins (IAPs) and has been implicated in the control of cell survival as well as regulation of mitosis in cancer, including solid tumors and hematological malignancies (27)(28)(29). Upon activation of pro-apoptotic cell signaling, survivin is released from the mitochondria and inhibits caspases-3 and -9.…”

“…Overexpression of suvivin is correlated with advanced disease, accelerated time of recurrence, reduced survival and resistance to therapy (30). AML patients with overexpression of survivin showed an unfavorable response to chemotherapy in 81.2% of the patients and shorter median survival time (30 days) compared to that of patients with normal expression (29). Thus, targeting survivin with small-molecule inhibitors by their anti-sense approaches or natural IAP antagonist mimetics may be an attractive strategy of anti-leukemia treatment.…”

In vitro anti-cancer activity of chamaejasmenin B and neochamaejasmin C isolated from the root of Stellera chamaejasme L

“…Human liver carcinoma cell lines (HepG2 and SMMC-7721), a human non-small cell lung cancer cell line (A549), human osteosarcoma cell lines (MG63, U2OS, and KHOS), a human colon cancer cell line (HCT-116) and a human cervical cancer cell line HeLa [16] were purchased from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China), and the genotypes were authenticated by DNA fingerprinting. The HepG2, HeLa, and HCT-116 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, and all other cell lines were cultured in RPMI-1640 supplemented with 10% fetal bovine serum.…”