Exogenous Morphine Inhibits Human Gastric Cancer MGC-803 Cell Growth by Cell Cycle Arrest and Apoptosis Induction

Qin, Yi; Chen, Jing; Li, Li; Liao, Chun-Jie; Liang, Yubing; Guan, Enjian; Xie, Yubo

doi:10.7314/apjcp.2012.13.4.1377

Cited by 33 publications

(23 citation statements)

References 30 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro studies have shown the pro-apoptotic action of morphine on cancer cells by different mechanisms including inhibition of NF-κB via nitric oxide [23,24] whereas other studies have shown inhibition of apoptotic processes via p53, a key factor in programmed cell death [25][26][27]. These findings are somewhat conflicting with data showing an inhibitory effect of morphine tumor cell proliferation in vitro [28][29][30][31].…”

Section: Mechanism Favoring Type Of Anesthesia and Cancer Recurrencecontrasting

confidence: 56%

Anesthetic Considerations for Management of Cancer Patients to Decrease Cancer Recurrence

Laudański¹,

Wei²,

Korley³

2016

J Anesth Clin Res

View full text Add to dashboard Cite

Alongside the known direct, short-term effects of anesthesia in general, there is emerging evidence of an immunomodulatory effect with specific anesthetics that may decrease patient's defences against malignant neoplastic growths. This effect is especially important in the setting of surgical management of neoplasms, which is often the best option for long-term survival in patients with solid neoplasm. Many studies have speculated on the best anesthetic technique to reduce the neoplasm recurrence and promote patient survival, however, we often neglect the sympathetic stress response to neoplasia and how anesthetics modulate this effect. In this review, we study the evidence as it pertains to anesthetic techniques and pain control, particularly general vs. regional anesthesia, and opioid analgesia. At this time there is not enough evidence to support that regional anesthesia has a more favorable outcome than general anesthesia, or that opioids should not be used in neoplasm-related pain management because of their potential pro-metastatic properties secondary to opioid-induced immunosuppression. Instead, the debate over anesthetic use should be centered on adequate pain control since overwhelming evidence have shown that pain-related stress reaction mediated via β-adrenergic activation, promotes neoplastic propagation and metastasis, hence decreasing survival rates.

show abstract

Section: Mechanism Favoring Type Of Anesthesia and Cancer Recurrencecontrasting

confidence: 56%

Anesthetic Considerations for Management of Cancer Patients to Decrease Cancer Recurrence

Laudański¹,

Wei²,

Korley³

2016

J Anesth Clin Res

View full text Add to dashboard Cite

show abstract

“…The analgesic potency of morphine given intra-articularly is comparable to that of bupivacaine 23 and fentanyl, 24,25 but data regarding the dose-ratio between drugs is heterogeneous. Morphine was reported to induce apoptosis and necrosis in cancer cell lines, 26 but it did not induce any cytotoxic effects in human ham-string progenitor cells. 8 Fentanyl was less cytotoxic than ropivacaine in mesenchymal stem cells.…”

Section: Discussionmentioning

confidence: 93%

Differential cytotoxic properties of drugs used for intra-articular injection on human chondrocytes

Stueber

Karsten²,

Stoetzer³

et al. 2014

European Journal of Anaesthesiology

View full text Add to dashboard Cite

show abstract

“…7) We also demonstrated that morphine could suppress the growth and the cell cycle progression of MGC-803 cells, the mechanism of which might be related to activation of caspase-3 and caspase-9, and inhibition of NF-κB nuclear translocation. 8) Some studies have found that at a clinical concentration morphine (0.01 µM) can induce cell apoptosis or necrosis in MCF-7 human tumor cell lines in vitro and inhibit the growth of tumor cells. 9) In addition, morphine (0.1 to 100 µM) and DAMGO (an μ-opioid receptor selective agonist) can inhibit the adhesive and migration of the HEK FLAG-MOP cells, the mechanisms of which are associated with mediation of an opioid receptor.…”

mentioning

confidence: 99%

Morphine Can Inhibit the Growth of Breast Cancer MCF-7 Cells by Arresting the Cell Cycle and Inducing Apoptosis

Chen

Qin

et al. 2017

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

Morphine is widely used for relieving cancer pain in patients with advanced cancer. However, whether morphine can suppress or promote the progression of cancer in breast cancer patients receiving morphine analgesia remains unclear. Therefore, we used an in vitro model treated with morphine and naloxone to investigate the effects of morphine on breast cancer cell line MCF-7. MCF-7 cells were cultured with different concentrations (0.01 to 10 µM) of morphine at 12th, 24th, 36th, 48th, 60th and 72nd hours. Then, cell viability was measured through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell cycle and apoptosis assays were detected by flow cytometry (FCM). In addition, cell proliferation was conducted by colony formation assay. In this study, we have found that morphine (0.01 to 10 µM) could significantly reduce the cell vitality, growth and colony formation rate of MCF-7 cells, which has a certain relationship with cell cycle progression arrested at the G0/G1 and G2/M phase and MCF-7 cells apoptosis. Moreover, naloxone along with morphine could not reverse these effects, which indicates that the inhibition of MCF-7 breast cancer cell growth and proliferation by morphine could be its independent effect, not associated with opioid receptors. Morphine can inhibit cell growth by blocking the cell cycle and promote apoptosis in MCF-7 cells. Hence, morphine may be unable to promote the progression of cancer in breast cancer patients receiving morphine analgesia.Key words apoptosis; breast cancer; cell cycle; MCF-7 cell; morphine Breast cancer (BC) is the most commonly diagnosed cancer among women.1,2) In addition, younger women often present with more aggressive incidence of BC and leading to cause high mortality rate of BC, between the ages of 20 and 59. 3,4) Furthermore, most of the cancer patients are severely stressed by cancer pain, which in turn creates further psychological burden and affects patients outcome. Therefore, cancer pain has increasingly become the major focus of attention and opioids candidates are routinely used to treat cancer pain.Morphine is the representative opioid and is widely used for treatment in advanced cancer. 5,6) Besides, its analgesic action, morphine has anti-nociceptive effect, and might even change tumor progression. In our earlier study, we found that opioid molecule fentanyl could inhibit the vitality of gastric carcinoma cells and cell cycle progression, the mechanism of which might be associated with inhibition of nuclear factor-kappaB (NF-κB) nuclear translocation and phosphatase and tensin homolog deleted from chromosome 10 (PTEN) tumor suppressor gene upregulation. 7) We also demonstrated that morphine could suppress the growth and the cell cycle progression of MGC-803 cells, the mechanism of which might be related to activation of caspase-3 and caspase-9, and inhibition of NF-κB nuclear translocation. 8)Some studies have found that at a clinical concentration morphine (0.01 µM) can induce cell apoptosis or necrosis in MCF-7 human tu...

show abstract

Exogenous Morphine Inhibits Human Gastric Cancer MGC-803 Cell Growth by Cell Cycle Arrest and Apoptosis Induction

Cited by 33 publications

References 30 publications

Anesthetic Considerations for Management of Cancer Patients to Decrease Cancer Recurrence

Anesthetic Considerations for Management of Cancer Patients to Decrease Cancer Recurrence

Differential cytotoxic properties of drugs used for intra-articular injection on human chondrocytes

Morphine Can Inhibit the Growth of Breast Cancer MCF-7 Cells by Arresting the Cell Cycle and Inducing Apoptosis

Contact Info

Product

Resources

About