Study of Surrogates for SW846 Method 8330

Ding, Jiefei; Xie, Kevin; Liu, Eddie; Zhang, Yongfeng; Hsia, Yu-Ping; Luo, Zi-ping

doi:10.1093/chromsci/37.3.65

Cited by 2 publications

(1 citation statement)

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“…Indeed, the use of surrogates for immobilization purposes has been seen previously in phage display for binding TNT peptides, as well as in the discovery of antibodies for targeting anthrax . The use of surrogates is a widely known procedure for investigating the detection of explosives, deadly pathogens, chemical weapons, and bioterrorist agents . This is due to their ease of chemical modification, binding, acquisition, and due to having a safer profile.…”

Section: Methodsmentioning

confidence: 99%

Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate‐a surrogate of PETN, via phage display technology

et al. 2017

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The present research investigates the identification of amino acid sequences that selectively bind to a pentaerythritol tetranitrate (PETN) explosive surrogate. Through the use of a phage display technique and enzyme-linked immunosorbent assays (ELISA), a peptide library was tested against pentaerythritol trinitrate hemisuccinate (PETNH), a surrogate of PETN, to screen for those with amino acids having affinity toward the explosive. The results suggest that the library contains peptides selective to PETNH. Following three rounds of panning, clones were picked and tested for specificity toward PETNH. ELISA results from these samples show that each phage clone has some level of selectivity for binding to PETNH. The peptides from these clones have been sequenced and shown to contain certain common amino acid segments among them. This work represents a technological platform for identifying amino-acid sequences selective toward any bio-chem analyte of interest.

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Section: Methodsmentioning

confidence: 99%

Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate‐a surrogate of PETN, via phage display technology

et al. 2017

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Nitroaromatics, Environmental Analysis of

Schmidt

Steinbach

Bütehorn

2000

Encyclopedia of Analytical Chemistry

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Nitroaromatic compounds (NACs) are important anthropogenic chemicals, which may enter all areas of the environment in significant amounts. Once there, they may be transformed, mainly via redox reactions. Most NACs and their transformation products are rather toxic, both in humans and ecosystems, and they are especially known for their carcinogenic potential. Thus, input and detrimental effects of NACs make environmental surveillance necessary, which in turn requires selective, sensitive, unbiased and robust methods suitable for the analysis of complex mixtures. These requirements are mainly fulfilled by the combination of an enrichment step with chromatographic separation and use of an appropriate detector. Official methods today use liquid–liquid extraction (LLE) or solid‐phase extraction (SPE), followed by high‐performance liquid chromatography (HPLC) with ultraviolet (UV) detection, or gas chromatography (GC) with electron capture detection (ECD). These methods are widely established and have been thoroughly tested in interlaboratory comparisons. However, new methods for extraction, separation and detection are emerging, which are superior in terms of the required characteristics, and thus may replace the existing methods in the future. Both standard methods and new developments for the environmental analysis of NACs are discussed in this article. Regarding the references given, priority was given to the most recent developments, and thus about 70% of the cited literature is less than five years old.

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Study of Surrogates for SW846 Method 8330

Cited by 2 publications

References 0 publications

Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate‐a surrogate of PETN, via phage display technology

Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate‐a surrogate of PETN, via phage display technology

Nitroaromatics, Environmental Analysis of

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