Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate‐a surrogate of PETN, via phage display technology

Rees, William D.; Caguiat, Jonathan; Asch, David K.; Fagan, Diana; Cortes, Pedro

doi:10.1002/bip.22997

Cited by 4 publications

(3 citation statements)

References 29 publications

(30 reference statements)

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“…Although phage display has been widely used to discover binders for large molecules [70,71], the literature is scarcer for smaller targets. Interesting examples are the detection of explosives [72][73][74][75] and pesticides [76,77]. Indeed, the screening of peptides selective to a VOC is particularly challenging, since it requires immobilizing the odorant molecule on a surface.…”

Section: Phage Displaymentioning

confidence: 99%

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

Hurot

Scaramozzino

Buhot

et al. 2020

Sensors

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Artificial noses are broad-spectrum multisensors dedicated to the detection of volatile organic compounds (VOCs). Despite great recent progress, they still suffer from a lack of sensitivity and selectivity. We will review, in a systemic way, the biomimetic strategies for improving these performance criteria, including the design of sensing materials, their immobilization on the sensing surface, the sampling of VOCs, the choice of a transduction method, and the data processing. This reflection could help address new applications in domains where high-performance artificial noses are required such as public security and safety, environment, industry, or healthcare.

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Section: Phage Displaymentioning

confidence: 99%

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

Hurot

Scaramozzino

Buhot

et al. 2020

Sensors

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“…There has been limited investigation in the literature for suitable mocks for PETN, in recent years using mocks to develop techniques for explosives trace detection [3][4][5]. For other HE materials, inert molecular crystals are often used as mocks in cases where handling HE is a concern [6].…”

Section: Introductionmentioning

confidence: 99%

A Thermal and Nanomechanical Study of Molecular Crystals as Versatile Mocks for Pentaerythritol Tetranitrate

et al. 2020

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Pentaerythritol tetranitrate (PETN) is a commonly used high explosive (HE) in detonators. Often, surrogate or “mock” materials are used in place of HE for mechanical tests, proofing out equipment, or developing new diagnostics. However, there is no commonly accepted mock for PETN. A good mock should match at least one physical property of the target material, and ideally mimic multiple thermal and mechanical properties. Here, we investigate several molecular crystals to evaluate their efficacy in mocking PETN density, melting point, elastic modulus, hardness, plastic deformation, and fracture behavior. Materials were tested with a combination of calorimetry and nanoindentation. Two materials, 2,4,6-trifluorobenzoic acid (246 TFBA) and mesoerythritol, were downselected for detailed indentation study after the initial round of screening experiments, both were found to mimic PETN mechanical behavior quite well, 246 TFBA closer to PETN in most properties (hardness, modulus, and density) than erythritol, but erythritol having advantages in relative cost and matching the onset of yield. Depending on the desired implementation of the mock, one material may be preferred over the other, but both have potential as generic mocks for PETN. Nanoindentation is demonstrated as a versatile tool to provide rapid screening of these materials’ mechanical properties.

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“…Techniques using library screening have improved other methods [ 19 , 25 ] to identify components as potential drugs against diseases. These new methods have hugely increased speed and throughput to identify interesting lead candidates.…”

Section: Introductionmentioning

confidence: 99%

Identification of Peptide Antagonists to Thioredoxin Glutathione Reductase ofSchistosoma japonicum

Song

Yin

et al. 2018

BioMed Research International

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Schistosomiasis is one of the world's major public health problems. Praziquantel is currently the only effective drug against schistosomiasis. As resistance of praziquantel has emerged in some endemic areas, development of new antischistosomal agents should be a high priority. In this study, a phage display peptide library was used for screening for peptide antagonists of thioredoxin glutathione reductase of Schistosoma japonicum (SjTGR), which has been identified as an alternative drug target. Three rounds of panning produced four different fusion phages. ELISA proved that all four phages could bind to SjTGR. One peptide, JIPDys1 (aa, WPHNWWPHFKVK), reduced enzyme activity of SjTGR by more than 50%. 2 μM of the synthesized peptide of JIPDys1 inhibited the activity of TrxR, GR, and Grx of SjTGR by 32.5%, 100%, and 100%, respectively. The IC50 values of the synthetic peptide JIPDys1 for TrxR, GR, and Grx were 3.67 μM, 0.11 μM, and 0.97 μM, respectively. Based on computer simulation, it appeared that JIPDys1 binds to the substrate binding sites of glutathione reductase (GR) and glutaredoxin (Grx). Our data show that the peptide, JIPDys1 (aa, WPHNWWPHFKVK), is a promising candidate to develop novel drugs against S. japonicum which acts by binding with SjTGR and reduces enzyme activity of SjTGR.

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Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate‐a surrogate of PETN, via phage display technology

Cited by 4 publications

References 29 publications

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

A Thermal and Nanomechanical Study of Molecular Crystals as Versatile Mocks for Pentaerythritol Tetranitrate

Identification of Peptide Antagonists to Thioredoxin Glutathione Reductase ofSchistosoma japonicum

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