Study of subretinal intercellular space

Lai, Yin-Lok; Masuda, Kohji; Hayasaka, Seiji; Lin, T C; Chang, Te Ning; Lug, Raymond; Wang, S J; Liu, Y P

doi:10.1016/0014-4835(82)90003-3

Cited by 8 publications

(1 citation statement)

References 11 publications

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“…A loss of melanin is seen during in vitro proliferation (Whittaker 1963), while spreading induces alterations in the cellular surface m g Irphology (Nicolaissen Jr et al 1982b), and both these mechanisms could contribute in the formation of the multilayered structures. The space between sensory retina and the RPE is filled with a matrix composed of mucopolysaccharides (Sidman 1958;Fine & Zimmerman 1963;Berman 1982) and proteins (Adler & Severin 1981;Lai et al 1982), arid transmission electron microscopy has identified a branching, chain-like, ruthenium red stainable component in this matrix (Feeney 1973). A recent investigation indicates, that this component could represent molecular connections between the two outer layers of retina (Nicolaissen Jr 1985).…”

Section: Discussionmentioning

confidence: 99%

Human retinal pigment epithelium in long term explant culture

Nicolaissen¹,

Allen

Nicolaissen

et al. 1986

Acta Ophthalmologica

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Explants of human retinal pigment epithelium were maintained in culture in various types of media, and examined by light and transmission electron microscopy. After one month in vitro, the central areas showed a monolayered configuration with distinct polarity and presence of ruthenium red stainable material on the apical surface. On the peripheral areas of Bruch's membrane, multilayered lesions were observed to develop and to extend from the monolayered epithelium and past the cut edge in Bruch's membrane. Cells in these lesions contained little melanin and generally lacked an apico-basal polarity. Ruthenium red staining revealed the presence of electron dense material on the apical surface of the lesions as well as in the extracellular space between cells in the various layers. Development of multilayered lesions with deposition of extracellular material are seen in various chorio-retinal disorders, including senile macular degenerations and also subsequent to laser and cryo-therapy. The findings in the present study point to the explant culture system as a valuable tool in the study of important aspects of chorio-retinal pathology.

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Section: Discussionmentioning

confidence: 99%

Human retinal pigment epithelium in long term explant culture

Nicolaissen¹,

Allen

Nicolaissen

et al. 1986

Acta Ophthalmologica

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Immunocytochemical demonstration of interphotoreceptor retinoid-binding protein in cerebellar medulloblastoma

Korf

Schachenmayr

et al. 1992

Acta Neuropathol

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Previously, immunoreactive rod-opsin and S-antigen (arrestin), two highly characteristic markers of retinal photoreceptors and pinealocytes, were shown to be present in certain medulloblastoma cells. It, thus, has been suggested that such cells differentiate along the photoreceptor lineage. This is corroborated in the present immunocytochemical investigation using antibodies against another photoreceptor-cell marker, the interphotoreceptor retinoid-binding protein (IRBP). As shown in preparations of human retina and pineal organ, IRBP can be successfully demonstrated in formalin-fixed and paraffin-embedded tissue: the IRBP immunoreaction is located to the outer and inner segments of retinal photoreceptor cells and to perikarya of certain pinealocytes. Examination of formalin-fixed, paraffin-embedded biopsy specimens of 66 cerebellar medullo-blastomas revealed varying numbers of IRBP-immuno-reactive tumor cells in 19 cases that were formerly shown to contain rod-opsin and S-antigen immunoreaction. IRBP-immunoreactive tumor cells were also found in a retinoblastoma and a pineocytoma, but not in neuroblastoma, ganglioneuroblastoma, glioblastoma, oligodendroglioma and astrocytoma. The results indicate: (1) cerebellar medulloblastomas are heterogeneous in their differentiation potential; (2) one type of medulloblastoma displays photoreceptor characteristics; (3) this type appears to be closely related to retinoblastoma and pineal cell tumors; and (4) all three types of tumors may display additional common features to be explored in future studies.

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