An extracellular retinol-binding glycoprotein in the rat eye—characterization, localization and biosynthesis

Gonzalez‐Fernandez, Federico; Landers, Robert A.; Glazebrook, Patricia A.; Fong, Shao-Ling; Liou, Gregory I.; Lam, Dominic M. K.; Bridges, C.D.B.

doi:10.1016/0197-0186(85)90179-2

Cited by 19 publications

(1 citation statement)

References 17 publications

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“…The interphotoreceptor retinoid-binding protein (IRBP), which is the most abundant protein in the interphotoreceptor matrix (IPM) [6][7][8][9][10], is one of the principal elements altered in early stages of diabetes. This protein is expressed mainly by the cone and rod photoreceptor cells [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Interphotoreceptor Retinoid-Binding Protein Implications in Diabetic Retinopathy

Bermea¹

2018

Early Events in Diabetic Retinopathy and Intervention Strategies

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The interphotoreceptor retinoid-binding protein (IRBP) is the most abundant protein in the interphotoreceptor matrix (IPM) and its levels decrease beginning in the early stages of diabetes. IRBP participates in the delivery of retinoids between retinal cells to carry out the visual cycle and also protects those retinoids against degradation in the IPM. IRBP deficiency is related to several conditions such as retinitis pigmentosa, cone-rod dystrophy, increased oxidative stress in the photoreceptors, and myopia. Decreased IRBP levels in diabetes could be due to the secretion of inflammatory cytokines and a direct effect of hyperglycemia on the photoreceptors. It is known that prior to the occurrence of vascular changes in diabetic retina, electrophysiological alterations occur on early potentials. Alterations on the photoreceptor outer segments and increased oxidative stress indicate an important affliction of the photoreceptors from early stages. Due to the importance of IRBP in photoreceptor wellness, its decreased levels may be linked to early photoreceptor affection. More studies are required to describe in detail the whole impact that decreased levels of IRBP in diabetes may have.

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Section: Introductionmentioning

confidence: 99%

Interphotoreceptor Retinoid-Binding Protein Implications in Diabetic Retinopathy

Bermea¹

2018

Early Events in Diabetic Retinopathy and Intervention Strategies

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Distribution of interstitial retinol‐binding protein (IRBP) in the vertebrates

et al. 1986

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Immunoblots of interphotoreceptor matrix preparations from 20 species belonging to six vertebrate classes were probed with antibodies against bovine interstitial retinol-binding protein (b-IRBP). Each preparation displayed an immunoreactive protein band. In the Osteichthyes, the apparent Mr of this band was 67,600 +/- 2,700 (mean +/- SD, n = 8). In two of the Osteichthyes, the band was resolved into a closely spaced doublet. Including previously published data for five mammals and one amphibian, species from the other classes (Chondrichthyes, one species; Amphibia, four species; Reptilia, one species; Aves, one species; Mammalia, nine species) had IRBPs with Mr that averaged 2.0 times that of the Osteichthyes, namely 134,200 +/- 8,600 (mean +/- SD, n = 17). Frog IRBP was very similar to mammalian IRBP in terms of its immunohistochemical distribution (determined with rabbit anti-frog IRBP antibodies), its molecular weight (sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel-filtration chromatography), retinol- and concanavalin A-binding ability, and because it was synthesized and secreted in vitro by the isolated retina but not by the pigmented layers of eye. Goldfish IRBP apparently binds exogenous (3H)-retinol but does not bind concanavalin A and has about half the Mr of frog IRBP. The occurrence of IRBP-like proteins cross-reacting with anti b-IRBP antibodies in the interphotoreceptor matrix of all six major vertebrate classes is consistent with the hypothesis that IRBP is an important element in the vertebrate visual cycle.

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Roles of Intra- and Extracellular Retinoid-Binding Proteins in the Visual Cycle

Bridges¹

1987

Cell and Developmental Biology of the Eye

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An extracellular retinol-binding glycoprotein in the rat eye—characterization, localization and biosynthesis

Cited by 19 publications

References 17 publications

Interphotoreceptor Retinoid-Binding Protein Implications in Diabetic Retinopathy

Interphotoreceptor Retinoid-Binding Protein Implications in Diabetic Retinopathy

Distribution of interstitial retinol‐binding protein (IRBP) in the vertebrates

Roles of Intra- and Extracellular Retinoid-Binding Proteins in the Visual Cycle

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