Study of specific IgG subclass antibodies for diagnosis of Gnathostoma spinigerum

Nuchprayoon, Surang; Sanprasert, Vivornpun; Suntravat, Montamas; Kraivichian, Kanyarat; Saksirisampant, Wilai; Nuchprayoon, Issarang

doi:10.1007/s00436-003-0947-x

Cited by 20 publications

(27 citation statements)

References 32 publications

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“…has been useful in the development of a diagnostic tool, as reported for G. spinigerum. 21,22,29 However, in the Pacific region of Mexico, species of Gnathostoma other than G. spinigerum and G. doloresi are responsible for the disease, specifically G. binucleatum. 25 Thus, antigens and immune responses observed in patients infected with other species of Gnathostoma could be different for those observed with patients infected with G. binucleatum.…”

Section: Discussionmentioning

confidence: 99%

“…21 However, it has also been reported that total IgG against G. spinigerum shows cross-reactivity with other parasites. 22 We investigated whether antigens of G. binucleatum recognized by total IgG showed any differences in recognition when each IgG subclass was tested by Western blotting. As shown in Table 1 patients with gnathostomiasis caused by G. binucleatum showed IgG1, IgG2, or IgG3 responses against antigens of this parasite.…”

Section: Discussionmentioning

confidence: 99%

“…21,22 Therefore, the purpose of this study was to characterize the humoral immune response to a crude extract of G. binucleatum in patients with clinical diagnoses of gnathostomiasis to detect a possible antibody class or subclass that could be used in the diagnosis of gnathostomiasis.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Zambrano‐Zaragoza¹,

Durán‐Avelar²,

Messina-Robles³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked freshwater fish infected with the advanced third-stage larvae of Gnathostoma spp. This disease is endemic to the Pacific region of Mexico, and one of its etiologic agents has been identified as Gnathostoma binucleatum. We characterized the humoral immune response of patients clinically diagnosed with gnathostomiasis by detecting total IgM, IgE, and IgG class and subclasses against a crude extract of the parasite by Western blotting. Our results do not show differences in the antigens recognized by IgM and IgE. However, we found that the specific humoral immune response is caused mainly by IgG, specifically IgG4. We found that 43%, 65.2%, 54.1%, and 26.3% of the patients recognize the 37-kD, 33-kD, 31-kD, and 24-kDa antigens, suggesting that the 33-kD antigen is the immunodominant antigen of G. binucleatum.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Zambrano‐Zaragoza¹,

Durán‐Avelar²,

Messina-Robles³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…comm). Some laboratories use an ELISA for immunoglobulin G subclasses as a screening test (12). In our fi rst patient, the larva was identifi ed based on its morphologic features, including the number and size of cephalic hooks and the character and extent of spines on its body (Figure 2) (2,13).…”

Section: Discussionmentioning

confidence: 99%

Gnathostomiasis Acquired by British Tourists in Botswana

Herman

Wall²,

Tulleken³

et al. 2009

Emerg. Infect. Dis.

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“…Posteriormente, se desarrolló una prueba ELISA para la detección de anticuerpos IgG en el tercer estadio de la larva, cuya sensibilidad, según los reportes, no es muy alta (59 a 87 %), en tanto que la especificidad fluctúa entre 79 y 96 %. Tiempo después, Nuchprayon, et al, demostraron que el uso de las subclases de IgG en lugar de la IgG total, mejoraba la sensibilidad y la especificidad de la prueba (27).…”

Section: Caso Clínicounclassified

Gnatostomiasis cutánea, primer caso confirmado en Colombia

et al. 2015

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: estudio patológico e identificación del espécimen Leonardo F. Jurado: contribución en la identificación del parasito, revisión de la historia clínica y de la literatura científica pertinente Todos los autores participaron en la escritura del manuscrito. La gnatostomiasis es una zoonosis parasitaria causada por algunas especies del género de helmintos Gnathostoma. La presentación clínica es muy variada y su diagnóstico constituye un desafío; es endémica en países tropicales y subtropicales, y la transmisión se asocia con el consumo de carne de animales acuáticos, cruda o mal cocida. En las últimas décadas, el creciente turismo internacional y el consumo de alimentos exóticos, han provocado un notable aumento de casos de la enfermedad. En nuestro país no se ha reportado ningún caso confirmado de gnatostomiasis. Se presenta el caso de un hombre colombiano de 63 años de edad, viajero frecuente al exterior, que consultó por presentar síntomas gastrointestinales. Durante la hospitalización desarrolló una lesión cutánea en el flanco abdominal derecho, de la cual se extrajo una larva cuya descripción morfológica permitió clasificarla como Gnathostoma spinigerum. Se trata de la primera confirmación de un caso importado de gnatostomiasis en Colombia. En este artículo se discuten las generalidades, la etiología, la patogenia y el tratamiento de la enfermedad, con especial énfasis en las características particulares de este paciente. Gnatostomiasis cutánea, primer caso confirmado en ColombiaPalabras clave: gnatostomiasis/diagnóstico, Gnathostoma, Larva migrans, parasitosis intestinales, zoonosis, Colombia. doi: http://dx.doi.org/10.7705/biomedica.v35i4.2547 Cutaneous gnathostomiasis, first confirmed case in ColombiaGnathostomiasis is a parasitic zoonosis caused by some species of helminthes belonging to the genus Gnathostoma. It has a wide clinical presentation and its diagnosis is a challenge. Tropical and subtropical countries are endemic, and its transmission is associated with eating raw or undercooked meat from fresh water animals. Increasing global tourism and consuming exotic foods have produced a noticeable rise in cases of the disease in the last decades. However, in our country, there has not been any confirmed case of gnathostomiasis previously reported. We present the case of a 63-year-old Colombian man with an international travel history, who presented with gastrointestinal symptoms. During the hospital stay, he developed a cutaneous lesion on the upper right abdominal quadrant, where later, a larva was found. A morphological study allowed us to identify it as Gnathostoma spinigerum. As such, this is the first report of an imported case of gnathostomiasis confirmed in Colombia. This article describes the principles, etiology, pathogenic cycle and treatment of this disease with special considerations to our patient's particular features.

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Study of specific IgG subclass antibodies for diagnosis of Gnathostoma spinigerum

Cited by 20 publications

References 32 publications

Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Gnathostomiasis Acquired by British Tourists in Botswana

Gnatostomiasis cutánea, primer caso confirmado en Colombia

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