Study of Regular Intracellular and Membrane Processes in Neurons by Laser Interference Microscopy

Abstract

Regular fluctuations in the height of phase profile of isolated neuron were studied by laser interference microscopy. The resultant spectra of frequencies of changes in the phase profile height indicate regular local changes in the optical density and/or geometry of neurons. The frequency spectra for the central and pri-membrane regions of the cells are different.

Unraveling Cell Processes: Interference Imaging Interwoven with Data Analysis

“…Experiments were carried out on the modulation interference microscope MIM 2.1 developed by the company 'Amphora Laboratories' (Russia) [31][32][33]. MIM 2.1 is based on the 'Linnik' interferometer with a laser power per cell of less than 1 mW.…”

“…Mast cells were isolated from male Wistar rats as described by Graevskaya et al [34]. Preparation of the pond snail and leech ganglia neurons were performed according to the procedure described in [10,33,35]. During the experiments, cells were placed in a containment chamber with a mirror bottom layer and with the following physiological solutions: buffer for erythrocytes (mM: 145 NaCl, 5 KCl, 1 CaCl 2 , 1 MgSO 4 , 4 Na 2 HPO 4 , 1 NaH 2 PO 4 , 10 glucose, pH 7.4), physiological solutions for rat mast cells (mM: 144 NaCl, 2.7 KCl, 4.6 Na 2 HPO 4 , 2 KH 2 PO 4 with addition of 100 mg of albumin, 0.5 ml of 1 M glucose solution and 0.1 ml of 1 M CaCl 2 solution before experiment) and physiological solutions for snail (mM: 50 NaCl, 1.5 KCl, 4 CaCl 2 , 1 MgCl 2 , 11 HEPES, pH 7.5) and leech (mM: 150 NaCl, 4 KCl, 1.8 CaCl 2 , 1 MgCl 2 , 11 HEPES, pH 7.4).…”

Non-invasive study of nerve fibres using laser interference microscopy

“…At present, phase images have been obtained for a variety of cell types, organelles and even whole organisms: neurons (Erokhova et al 2005;Rappaz et al 2005;Sosnovtseva et al 2005;Brazhe et al 2006;Yusipovich et al 2006), erythrocytes (Popescu et al 2004(Popescu et al , 2005Brazhe et al 2006), mast cells (Brazhe et al 2006), HeLa cells (Popescu et al 2004;Choi et al 2007), epithelial kidney cells (Park et al 2006), chloroplasts (Tychinsky et al 2004) and the nematode Caenorhabditis elegans (Choi et al 2007). Studies of intracellular dynamics by means of interference microscopy are less numerous.…”

A simple device for microinjections, manipulations and measurements using an electromorphological chip under mi-crointerferometric control of the interface and membrane processes at the thickness range of 5-1000 nm at different angles

“…2) водной проницаемости и осмотических явлений в мембранных структурах [43] 11) нейрофизиологических внутриклеточных и мембранных процессов на уровне нейронов в развитии и реакции, и их формирующегося в ре-зультате этой активности коннектома [59].…”