Study of polymorphic variable-number of tandem repeats loci in the ECOR collection and in a set of pathogenic Escherichia coli and Shigella isolates for use in a genotyping assay

Lindstedt, Bjørn-Arne; Brandal, Lin Thorstensen; Aas, Lena; Vardund, Traute; Kapperud, Georg

doi:10.1016/j.mimet.2007.01.001

Cited by 104 publications

(168 citation statements)

References 17 publications

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“…The genotyping assay of VNTR was performed on six polymorphic loci (CVN15, CVN7, CVN4, CVN1, CVN2, CVN14) (Lindstedt, Brandal, Aas, Vardund, & Kapperud, 2007) using a genetic analyser Applied Biosystems 7330 xl .…”

Section: Methodsmentioning

confidence: 99%

VIM‐1 carbapenemase‐producing Escherichia coli in gulls from southern France

Vittecoq

Laurens

Brazier

et al. 2017

Ecology and Evolution

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Acquired carbapenemases currently pose one of the most worrying public health threats related to antimicrobial resistance. A NDM‐1‐producing Salmonella Corvallis was reported in 2013 in a wild raptor. Further research was needed to understand the role of wild birds in the transmission of bacteria resistant to carbapenems. Our aim was to investigate the presence of carbapenem‐resistant Escherichia coli in gulls from southern France. In 2012, we collected 158 cloacal swabs samples from two gull species: yellow‐legged gulls (Larus michahellis) that live in close contact with humans and slender‐billed gulls (Chroicocephalus genei) that feed at sea. We molecularly compared the carbapenem‐resistant bacteria we isolated through culture on selective media with the carbapenem‐susceptible strains sampled from both gull species and from stool samples of humans hospitalized in the study area. The genes coding for carbapenemases were tested by multiplex PCR. We isolated 22 carbapenem‐resistant E. coli strains from yellow‐legged gulls while none were isolated from slender‐billed gulls. All carbapenem‐resistant isolates were positive for bla VIM ‐1 gene. VIM‐1‐producing E. coli were closely related to carbapenem‐susceptible strains isolated from the two gull species but also to human strains. Our results are alarming enough to make it urgently necessary to determine the contamination source of the bacteria we identified. More generally, our work highlights the need to develop more bridges between studies focusing on wildlife and humans in order to improve our knowledge of resistant bacteria transmission routes.

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Section: Methodsmentioning

confidence: 99%

VIM‐1 carbapenemase‐producing Escherichia coli in gulls from southern France

Vittecoq

Laurens

Brazier

et al. 2017

Ecology and Evolution

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“…We are, however, aware that the methodological uncertainties may be regarded as a weakness of our study. It has already been proven that the original 7-locus version of the MLVA-assay used in this study is able to type all ECOR strains, and as expected, does not assign them to the main phylogenetic groups defined by MLEE [17]. However, the recently updated version was found to type multidrug resistant E. coli lineages [40], and MLVA has been used for phylotyping STEC O157 [41].…”

Section: Mtc-h (11 Strains)mentioning

confidence: 53%

“…MLVA has a methodological drawback when the VNTR is present within a coding region or promoter and can thus be too discriminatory [42]. In the MLVA-assay used in this study, two of the VNTRs are located in gen-coding areas [17]. Similar studies have been performed, focusing on phylogeny and virulence factors, however, without concentrating on patient age, and with negative results regarding associations with outcome.…”

Section: Mtc-h (11 Strains)mentioning

confidence: 94%

“…The isolates were MLVA genotyped in accordance with the previously described protocols [16,17]. The following loci were included: CVN001, CVN002, CVN003, CVN004, CVN007, CVN014, CVN015, CVN016, and CVN017.…”

Section: Mlva Analysismentioning

confidence: 99%

“…By applying a 9-locus variant of the recently updated version [16] of the original protocol [17], the aim of the study was to determine if high age and age-linked clinical phenomena were associated with certain genotypes of E. coli found in bacteremia cases already well described [18]. Furthermore, we aimed to determine whether particular bacterial genotypes or antimicrobial resistance represented risk factors for hospital mortality.…”

mentioning

confidence: 99%

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SpringerBriefs in Food, Health, and Nutrition

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Pages: vi + 258 ISBN: Book: 978-1-910190-77-7. Ebook: 978-1-910190-78-4 £159, $319 Published by: Caister Academic Press www.caister.com Escherichia coli is an important member of the normal healthy microbiome of humans and other mammals. In addition, some strains are thought to be probiotic, and therefore beneficial to the host. However, other strains of E. coli have evolved into highly versatile, and frequently deadly, pathogens, the resultant diseases causing significant economic loss and public health burdens worldwide. Recent studies have shown that the E. coli genome has a high plasticity allowing it to adapt to new niches and survive in stressful conditions and to evolve into new hybrid strains with shared genes, including virulence genes. Omics and whole genome sequencing approaches have transformed research in this field allowing fascinating new insights into the molecular and cellular biology of the bacterium thus paving the way for the development of novel therapeutic strategies. Under the expert guidance of the editors in this book, renowned international authors provide timely and up-to-date reviews of current cutting-edge E. coli omics, molecular-and cellular-biology research. Topics range from E. coli genome plasticity and evolution to the application of omics technologies for in silico modeling to understand stress-triggered physiological responses. This authoritative volume is essential reading for scientists, both experts and students, working on pathogenic E. coli in academia, government, and biotechnology companies. It is also a must-read for anyone with an interest in bacterial pathogenesis and an important acquisition for all microbiology libraries.

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Study of polymorphic variable-number of tandem repeats loci in the ECOR collection and in a set of pathogenic Escherichia coli and Shigella isolates for use in a genotyping assay

Cited by 104 publications

References 17 publications

VIM‐1 carbapenemase‐producing Escherichia coli in gulls from southern France

VIM‐1 carbapenemase‐producing Escherichia coli in gulls from southern France

E. coli Bacteremia Strains - High diversity and Associations with Agerelated Clinical Phenomena

Detection and Typing Strategies for Pathogenic Escherichia coli

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