Study of polarization colors in the connective tissue wall of odontogenic cysts using picrosirius red stain

Shetty, Anusha; Tamgadge, Avinash; Bhalerao, Sudhir; Periera, Treville; Tamgadge, Sandhya; Gotmare, Swati

doi:10.4103/0975-8844.169771

Cited by 5 publications

(1 citation statement)

References 2 publications

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“…In the musculoskeletal system, severe burn induces hypercatabolism in muscle and bone due to the activation of systemic inflammation, and disuse from long immobilization periods ( 2 – 6 ). Muscle loss and atrophy postburn have been associated with increased levels of TNF-α ( 7 ), whereas bone mass loss has been associated with circulating TNF-α, IL-1β, and IL-6, which are increased shortly after burn ( 8 – 10 ). Despite the findings of mass loss in both skeletal muscle and bone, there are limited studies on the molecular and structural effects of burn injury on tendon.…”

Section: Introductionmentioning

confidence: 99%

Severe Burn-Induced Inflammation and Remodeling of Achilles Tendon in a Rat Model

Hernández

Buller

Mitchell

et al. 2018

Shock

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Severe burn causes systemic inflammation and hypercatabolism, resulting in damage to multiple organs distant to the burn site, including the musculoskeletal system. Bone mass and muscle loss have been reported. However, tendon that connects bone and muscle has not been studied in comparable detail. Here we aimed to characterize the molecular and functional changes in Achilles tendon triggered by severe burn. Forty male Sprague–Dawley rats received 40% total body surface area scald burn. Achilles tendons were collected up to 14 days postburn. Sham-treated animals served as a control group. We analyzed tendons for changes in expression of IL-6, IL-1β, TNF, MMP9, MMP13, TGFβ1, Collagens I and III, and for morphological and biomechanical changes. Gene expression of IL-6 and IL-1β as well as MMP9 and MMP13 increased in rat tendon 3 days after burn. Col3a1 increased at day 3 and col1a1 at day 7. At day 14, TGFβ1 increased, whereas the protein ratio for collagens I/III decreased, indicating tendon remodeling. Histological analysis with H&E and Picrosirius red staining further revealed a decrease in organized collagen fibers 14 days after burn. Biomechanical analysis showed a decrease in stiffness and ultimate force of tendons in burn rats.We conclude that tendinopathy was observed in Achilles tendon 14 days after severe burn, via the induction of inflammation and remodeling. The present study provides a model of tendinopathy that may be used for the development of therapeutic approaches after burn.

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Section: Introductionmentioning

confidence: 99%

Severe Burn-Induced Inflammation and Remodeling of Achilles Tendon in a Rat Model

Hernández

Buller

Mitchell

et al. 2018

Shock

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Stromal Changes In Benign and Malignant Salivary Gland Tumors With Picrosirius Red-A Polarizing Microscopic Study

Gajdhar¹,

Gajdhar²,

Patil³

et al. 2019

Indian Journal of Science and Technology

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Collagen and Its Role in predicting the Biological Behavior of Odontogenic Lesions

Kulkarni

Kumari

Jahagirdar

et al. 2017

The Journal of Contemporary Dental Practice

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Introduction: Odontogenic cysts and tumors have variable recurrence rates. Recurrence rate is mainly due to the activity of the epithelium. The epithelium of these lesions has been investigated extensively in regard to their role in proliferative and aggressive behavior of the lesions. However, the role of the connective tissue wall in their behavior has not been studied as extensively. Collagen is an essential part of the connective tissue as a whole and fibrous wall of cystic lesions especially. It is demonstrated by picrosirius red dye staining combined with polarization microscopy. This method permits the evaluation of the nature of the collagen fibers in addition to their thickness. Materials and methods:A total of 56 histopathologically diagnosed cases comprising odontogenic follicle, dentigerous cyst, unicystic ameloblastoma, keratocystic odontogenic tumor (KCOT), multicystic/solid ameloblastoma, and ameloblastic carcinoma were taken and stained using picrosirius red stain and evaluated using a polarizing microscope.Results: Collagen fibers in odontogenic follicles and dentigerous cysts showed predominant orange-red birefringence; fibers in unicystic ameloblastoma and KCOT showed both orange red and greenish-yellow birefringence; and fibers of multicystic/ solid ameloblastoma showed predominant greenish-yellow birefringence and ameloblastic carcinoma that showed almost complete greenish birefringence. As the biological behavior of the lesions in the spectrum studied progress toward aggressive nature, increase in immature collagen fibers is noticed. Conclusion:This study suggests that the nature of collagen fibers plays a pivotal role in predicting the biological behavior of odontogenic lesions. Clinical significance: Aggressive nature of the odontogenic lesions is determined by both the epithelium and the connective tissue components (collagen). Studying the nature and type of collagen helps in predicting its biological behavior. Collagen and Its Role in predicting the

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Study of polarization colors in the connective tissue wall of odontogenic cysts using picrosirius red stain

Cited by 5 publications

References 2 publications

Severe Burn-Induced Inflammation and Remodeling of Achilles Tendon in a Rat Model

Severe Burn-Induced Inflammation and Remodeling of Achilles Tendon in a Rat Model

Stromal Changes In Benign and Malignant Salivary Gland Tumors With Picrosirius Red-A Polarizing Microscopic Study

Collagen and Its Role in predicting the Biological Behavior of Odontogenic Lesions

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