Study of physically adsorbed stationary phases for open tubular capillary electrochromatography

Liu, Zhen; Zou, Hanfa; Ye, Mingliang; Ni, Jincheng; Zhang, Yukui

doi:10.1002/(sici)1522-2683(19991001)20:14<2891::aid-elps2891>3.0.co;2-l

Cited by 54 publications

(19 citation statements)

References 39 publications

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“…The immobilization of proteins such as BSA [495] or AAG [496] via silanes directly onto the capillary wall represents a simple way of preparing open tubular capillaries. Liu et al [497] simply adsorbed proteins, peptides and amino acids on the capillary wall by rinsing the capillary with a buffer containing the selector. These phases were evaluated by means of amino acids and some other compounds.…”

Section: Open Tubular Capillariesmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

225

135

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This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.

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Section: Open Tubular Capillariesmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

225

135

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“…(i) In open tubular CEC [236] chiral selectors, such as CD, chiral lactone and naphthylethylamine [237], and proteins [238] [239,240] reported on a chiral CEC column prepared by simple physical adsorption of a basic protein, i.e., cytochrome c or lysozyme, or a basic peptide to the capillary wall. Several derivatised amino acids were separated (R s 1.74± 2.05).…”

Section: Cecmentioning

confidence: 99%

Recent developments in chiral capillary electrophoresis and applications of this technique to pharmaceutical and biomedical analysis

Ahmad

2001

Electrophoresis

186

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This paper provides an overview of the current status of chiral capillary electrophoresis (CE). The emphasis is placed on the application of CE in chiral separation of various racemic compounds. During the last two years about 280 papers, several review articles, and two entire issues, edited by S. Fanali (Electrophoresis 1999, 20, 2577-2798, and H. Nishi and S. Terabe (J. Chromatogr. A 2000, 879, 1-471.) have been devoted to chiral CE. Enantiomeric separations of various compounds, e.g., pharmaceuticals, drug candidates, drugs and related metabolites in biological fluids, amino acids, di- and tri peptides, pesticides and fungicides, have been performed using different chiral selectors. Native and derivatized cyclodextrins continue to be the most widely used chiral selectors. Other chiral selectors such as natural and synthetic chiral micelles, crown ethers, chiral ligands, proteins, oligo- and polysaccharides, and macrocyclic antibiotics have also been applied to chiral CE separations.

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“…The application of this new separation technique for enantioseparation has recently been reviewed [22][23][24]. In CEC, the separation of enantiomers is accomplished by using open-tubular column formats with surface-confined CSP layer [25][26][27][28][29], by the use of capillaries either packed with chirally modified particulate stationary phases [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49], or with columns containing a continuous CSP mostly referred to as chiral monoliths [50][51][52][53][54][55][56][57][58][59][60][61][62].…”

Section: Introductionmentioning

confidence: 99%

Low-molecular-weight chiral cation exchangers: Novel chiral stationary phases and their application for enantioseparation of chiral bases by nonaqueous capillary electrochromatography

et al. 2002

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Cation exchange type chiral stationary phases (CSPs) based on 3,5-dichlorobenzoyl amino acid and amino phosphonic acid derivatives as chiral selectors (SOs) and silica as chromatographic support were developed and applied to enantiomer separations of chiral bases by nonaqueous capillary electrochromatography (NA-CEC). As a rationale for efficient CSP development we adopted the combined use of the "reciprocity principle of chiral recognition" and nonaqueous ion-pair CE as screening assay. Thus, (S)-atenolol was employed as chiral counter-ion added to the BGE in CE and a series of N-derivatized amino acids and amino phosphonic acids were screened to derive reciprocally information on their chiral recognition abilities for atenolol enantiomers. Two SO candidates, namely N-(3,5-dichlorobenzoyl)-O-allyl-tyrosine and N-(4-allyloxy-3,5-dichlorobenzoyl)-1-amino-3-methylbutane phosphonic acid that have been identified as potential SOs in the CE screening were, after immobilization on thiol-modified silica, evaluated in cation-exchange NA-CEC. The strong chiral cation exchanger with the free phosphonic acid group exhibited enhanced enantioselectivity compared to the weak chiral cation exchanger with the carboxylic acid group. A wide variety of chiral bases could be successfully resolved on the strong chiral cation exchanger with alpha-values up to 2.2 and efficiencies up to 375000 m-1 including beta-blockers and other amino alcohols, local anesthetics like etidocaine, antimalarial agents like mefloquine, Tröger's base, phenothiazines like promethazine, and antihistaminics. The influence of several experimental parameters (electrolyte concentration, acid-base ratio and acetonitrile-methanol ratio) was evaluated.

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Study of physically adsorbed stationary phases for open tubular capillary electrochromatography

Cited by 54 publications

References 39 publications

Chiral separation by chromatographic and electromigration techniques. A Review

Chiral separation by chromatographic and electromigration techniques. A Review

Recent developments in chiral capillary electrophoresis and applications of this technique to pharmaceutical and biomedical analysis

Low-molecular-weight chiral cation exchangers: Novel chiral stationary phases and their application for enantioseparation of chiral bases by nonaqueous capillary electrochromatography

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