Study of phylogenetic relationship of Turkish species of Klasea (Asteraceae) based on ISSR amplification

Abstract

Klasea is a taxonomically complex genus in which there are many problems, mostly with Klasea kotschyi and Klasea hakkiarica. It is challenging to differentiate the genera based on morphological characters alone. Revision studies performed on the basis of molecular data obtained from studies conducted in recent years have made the phylogenetic relationships and systematic positions of the taxa more apparent and reliable. In this study, Klasea, Serratula, Jurinea and Centaurea species native to Turkey, were collected from different localities of Anatolia and DNA was isolated from the collected samples. The data were analyzed ordination analyses including UPGMA and PCA using NTSYSpc 2.1. The infrageneric and intergeneric phylogenetic relationships between Klasea and other related genera were also characterized. The Klasea species were grouped into three clusters. It was determined that taxa Klasea kotschyi and Klasea hakkiarica are separate but closely related. Moreover, it was observed that the Klasea lasiocephala a separate group within the genera. Clearly the genera Klasea, Serratula, Jurinea and Centaurea are phylogenetically differentiated on the dendogram.

Systematic importance of achene macro‐micromorphological characteristics in selected species of the genera Crupina, Jurinea, and Klasea (Asteraceae) from Turkey

“…The genus has 46 species in 10 sections (Doğan, Duran, & Coşkun, 2014; Martins, 2006). It is distributed mainly to Himalayas, Central Asia, Mediterranean basin, SE Europe, China, southern Russia, Iran, and Turkey (Doğan, Duran, Şeker, Çetin, & Martin, 2015; Susanna, Garcia‐Jacas, Hidalgo, Vilatersana, & Garnatje, 2006).…”

Evaluation of genetic variability and relatedness among eight Centaurea species through CAAT-box derived polymorphism (CBDP) and start codon targeted polymorphism (SCoT) markers

“…Moreover, yildirim et al These few studies, however, did not include any of the Centaurea species found in Egypt in their analysis and used less reproducible marker systems (RAPD, SSR, ISSR etc.) [36][37][38][39]. With the evolution of many improved novel marker techniques, currently, they have become a not preferred marker technique in plant genetic diversity studies.…”

Genetic diversity of Central Javanese duck (Indonesia) based on Inter Simple Sequence Repeat markers

“…The ISSR is a polymerase chain reaction (PCR) -primarily based technique, using a single primer of microsatellite (repeat of dinucleotide, trinucleotide, tetranucleotide, or pentanucleotide). ISSR has several advantages, because the protocol is relatively simple, rapid, low-cost (Dogan et al 2015) and does not require sequence information for primer design (Son et al 2012). ISSRs can also be utilized for gene and population genetic mapping, as well as to recognize the breeds.…”