Study of mutual dependence of periodontal and colonic microbiome in health and pathology using NSG-sequencing

Петрухина, Н Б; Зорина, О А; Ших, Е. В.; Шибаева, А. В.; Шевелев, А. Б.

doi:10.17116/stomat20169528-13

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…segnis . Aggregatibacte r contains the aforementioned three species which was previously implicated as the periodontal pathogen [ 19 ]. This phenomenon needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

The microbial community shifts of subgingival plaque in patients with generalized aggressive periodontitis following non-surgical periodontal therapy: a pilot study

Han

Wang

2016

Oncotarget

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The object of this study is to characterize the bacterial community of subgingival plaque of two subjects with generalized aggressive periodontitis (GAgP) pre- and post-treatment. We picked two patients with GAgP and used high-throughput 16S rDNA sequencing. V4 hypervariable region was picked for PCR amplification of subgingival samples. Then, the PCR products were sequenced through Illumina MiSeq platform. One month after therapy, both the clinical features and periodontal parameters improved obviously. Moreover, the composition and structure of subgingival bacterial community changed after initial periodontal therapy. Also, the composition of the subgingival microbiota was highly individualized among different patients. Bacteroidetes, Spirochaetes and Fusobacteria were related to pathogenicity of GAgP while Actinobacteria and Proteobacteria seemed associated with clinical symptoms resolution. In this study, we found the subgingival bacterial community was high in species richness but dominated by a few species or phylotypes, with significant shifts of microbiota that occurred after treatment. This study demonstrated the shift of the subgingival bacterial community before and after treatment by high-throughput 16S rDNA sequencing, and provided a concise method for analysis of microbial community for periodontal diseases.

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“…segnis . Aggregatibacte r contains the aforementioned three species which was previously implicated as the periodontal pathogen [ 19 ]. This phenomenon needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

The microbial community shifts of subgingival plaque in patients with generalized aggressive periodontitis following non-surgical periodontal therapy: a pilot study

Han

Wang

2016

Oncotarget

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“…Therefore, it was suggested from this study that Lactobacillus reuteri and Capnocytophaga gingivalis might have antagonism effect. Prevotella baronia was found to be a relative of Prevotella intermedia [34]. Prevotella intermedia is a well-known periodontal pathogen and contributes a lot to the progress of pregnancy related gingivitis and periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Pregnancy-Related Ecological Shifts of Salivary Microbiota and its Association with Salivary Sex Hormones

Niu

Jiang

Gao

et al. 2020

Preprint

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Background: The interactions between hosts, oral microbiomes and microenvironments have been the subject of much research in recent years. Yet, whether the alterations in the host impact the oral microbiome is not understood well. The fluctuation of sex hormone levels during pregnancy is a dramatic change in the host, and is closely related to pregnancy-associated gingivitis. In this study, salivary estradiol and progesterone level were measured at three trimesters of pregnancy and after delivery (t1: ≤ 14weeks；t2: 20–25 weeks；t3: 33–37 weeks; t4: 42 days after delivery) from 11 pregnant and 7 non-pregnant volunteers, and their salivary microbiome were collected and profiled by 16S rDNA gene sequencing. Results: The diversity of the salivary microbiome increased significantly in t3, compared to the t1 (P<0.05), and a close parallel to the shift is found in the elevation of salivary sex hormones. Addionally, Capnocytophaga gingivalis, Peptoniphilus sp.oral taxon 386, Prevotella baroniae, Simonsiella muelleri and Lactobacillus reuteri were correlated to the fluctuation of sex hormone levels. Conclusions: The diversity of the salivary microbiome in pregnant women was elevated with the gestation weeks, which was found parallel to the changes in estradiol and progesterone levels in saliva. Rather than highly abundant bacteria, the low abundant bacteria were more vulnerable to the host impact.

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“…In a series of in vitro experiments testing the effect of BP on oral and non-oral bacterial strains, the BPs IBN, PAM, and ZOL were found to inhibit a range of bacterial species, including A. actinomycetemcomitans, C. ochracea, and C. rectus, 125 common periodontal pathogens. Perturbation of the commensal microbiota in the gut with agents such as BPs and antibiotics could represent a consideration in the influence of immune crosstalk 126,127 and hence dysbiosis contributing to ONJ pathology. Intervening at the level of microbe-host immune interactions supports the approach of managing infection with antibiotics and antimicrobials to aid resolution of ONJ.…”

Section: Microbiota and Inflammationmentioning

confidence: 99%

Mitigating osteonecrosis of the jaw (ONJ) through preventive dental care and understanding of risk factors

et al. 2020

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It is well established that alterations in phosphate metabolism have a profound effect on hard and soft tissues of the oral cavity. The present-day clinical form of osteonecrosis of the jaw (ONJ) was preceded by phosphorus necrosis of the jaw, ca. 1860. The subsequent removal of yellow phosphorus from matches in the early 20th century saw a parallel decline in “phossy jaw” until the early 2000s, when similar reports of unusual jaw bone necrosis began to appear in the literature describing jaw necrosis in patients undergoing chemotherapy and concomitant steroid and bisphosphonate treatment. Today, the potential side effect of ONJ associated with medications that block osteoclast activity (antiresorptive) is well known, though the mechanism remains unclear and the management and outcomes are often unsatisfactory. Much of the existing literature has focused on the continuing concerns of appropriate use of bisphosphonates and other antiresorptive medications, the incomplete or underdeveloped research on ONJ, and the use of drugs with anabolic potential for treatment of osteoporosis. While recognizing that ONJ is a rare occurrence and ONJ-associated medications play an important role in fracture risk reduction in osteoporotic patients, evidence to date suggests that health care providers can lower the risk further by dental evaluations and care prior to initiating antiresorptive therapies and by monitoring dental health during and after treatment. This review describes the current clinical management guidelines for ONJ, the critical role of dental-medical management in mitigating risks, and the current understanding of the effects of predominantly osteoclast-modulating drugs on bone homeostasis.

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Study of mutual dependence of periodontal and colonic microbiome in health and pathology using NSG-sequencing

Cited by 3 publications

References 12 publications

The microbial community shifts of subgingival plaque in patients with generalized aggressive periodontitis following non-surgical periodontal therapy: a pilot study

The microbial community shifts of subgingival plaque in patients with generalized aggressive periodontitis following non-surgical periodontal therapy: a pilot study

Pregnancy-Related Ecological Shifts of Salivary Microbiota and its Association with Salivary Sex Hormones

Mitigating osteonecrosis of the jaw (ONJ) through preventive dental care and understanding of risk factors

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