Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy

Liu, Jinyun; Qu, Yingmin; Wang, Guoliang; Wang, Xinyue; Zhang, Wenxiao; Li, Jingmei; Wang, Zuobin; Li, Dayou; Jiang, Jinlan

doi:10.1002/jemt.22950

Cited by 4 publications

(3 citation statements)

References 48 publications

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“…For mononuclear cells, the distribution of those parameters was significantly different. The authors observed that multinuclear cells are more resistant to the fullerenol when compared with the mononuclear ones [201].…”

Section: Mechanobiology In Crc Therapeuticsmentioning

confidence: 97%

Mechanobiology of Colorectal Cancer

Brás

Sousa

Carneiro

et al. 2022

Cancers

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In this review, the mechanobiology of colorectal cancer (CRC) are discussed. Mechanotransduction of CRC is addressed considering the relationship of several biophysical cues and biochemical pathways. Mechanobiology is focused on considering how it may influence epithelial cells in terms of motility, morphometric changes, intravasation, circulation, extravasation, and metastization in CRC development. The roles of the tumor microenvironment, ECM, and stroma are also discussed, taking into account the influence of alterations and surface modifications on mechanical properties and their impact on epithelial cells and CRC progression. The role of cancer-associated fibroblasts and the impact of flow shear stress is addressed in terms of how it affects CRC metastization. Finally, some insights concerning how the knowledge of biophysical mechanisms may contribute to the development of new therapeutic strategies and targeting molecules and how mechanical changes of the microenvironment play a role in CRC disease are presented.

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Section: Mechanobiology In Crc Therapeuticsmentioning

confidence: 97%

Mechanobiology of Colorectal Cancer

Brás

Sousa

Carneiro

et al. 2022

Cancers

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“…For example, by combining AFM and confocal fluorescence microscopy, information on the mechanical properties of cells and tissues and fluorescence images can be obtained simultaneously 189 . Because AFM can detect changes in the mechanical properties of cells, it be used to observe the difference in cells before and after drug action, and play an important role in drug toxicity testing, drug development and drug screening [190][191] .…”

Section: Summary and Prospectsmentioning

confidence: 99%

Application of atomic force microscope in diagnosis of single cancer cells

Wang

2020

Biomicrofluidics

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Changes in mechanical properties of cells are closely related to a variety of diseases. As an advanced technology on the micro/nano scale, atomic force microscopy is the most suitable tool for information acquisition of living cells in human body fluids. AFMs are able to measure and characterise the mechanical properties of cells which can be used as effective markers to distinguish between different cell types and cells in different states (benign or cancerous). Therefore, they can be employed to obtain additional information to that obtained via the traditional biochemistry methods for better identifying and diagnosing cancer cells for humans, proposing better treatment methods and prognosis, and unravelling the pathogenesis of the disease. In this report, we review the use of AFMs in cancerous tissues, organs, and cancer cells cultured in vitro to obtain cellular mechanical properties, demonstrate and summarize the results of AFMs in cancer biology, and look forward to possible future applications and the direction of development.

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“…The morphology and biomechanics of cells are important characteristics for distinguishing normal and damaged cells (Iyer et al, 2009; Lee & Lim, 2007; Murphy et al, 2013). In contrast to optical and electron microscopy, atomic force microscopy (AFM) can collect three‐dimensional images under near‐physiological conditions, enabling analysis of the morphology and mechanical properties of cells at the nanoscale (Balasuriya et al, 2012; Benitez & Toca‐herrera, 2014; He et al, 2018; Krieg et al, 2019; Liu et al, 2018; Rico et al, 2011). Cell biophysical properties are measured using AFM and recording the force–distance curve.…”

Section: Introductionmentioning

confidence: 99%

Attenuation of alcohol‐induced hepatocyte damage by ginsenoside Rg1 evaluated using atomic force microscopy

Zhang

Weng

Wang

et al. 2023

Microscopy Res & Technique

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Alcoholic liver disease is an important cause of death worldwide. Hepatocyte apoptosis is commonly observed in alcoholic liver disease. In this study, we investigated the effect of ginsenoside Rg1 (G-Rg1), an organic component of ginseng, on the alcoholinduced morphological and biophysical properties of hepatocytes. Human hepatocytes (HL-7702) were treated in vitro with alcohol and G-Rg1. The cell morphology was observed using scanning electron microscopy. Cell height, roughness, adhesion, and elastic modulus were detected using atomic force microscopy. We found that alcohol significantly induced hepatocyte apoptosis, whereas G-Rg1 attenuated the alcohol-induced hepatocyte damage. Scanning electron microscopy revealed that alcohol-induced significant morphological changes in hepatocytes, including decreased cell contraction, roundness, and pseudopods, whereas G-Rg1 inhibited these negative changes. Atomic force microscopy revealed that alcohol increased the cell height and decreased the adhesion and elastic modulus of hepatocytes. Following treatment with G-Rg1, the cell height, adhesion, and elastic modulus of alcoholinjured hepatocytes were all similar to those of normal cells. Thus, G-Rg1 can attenuate the alcohol-induced damage to hepatocytes by modulating the morphology and biomechanics of the cells. Research Highlights• In this study, the morphological characteristics of hepatocytes were observed using SEM.• The changes in hepatocyte three-dimensional images and biomechanical action caused by alcohol and G-Rg1 were examined at the nanoscale using AFM under near-physiological conditions.• Alcohol-induced hepatocytes showed abnormal morphology and biophysical properties. G-Rg1 attenuated the alcohol-induced damage to hepatocytes by modulating the morphology and biomechanics of the cells.

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Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy

Abstract: This article studies the morphological and mechanical features of multinuclear and mononuclear

Cited by 4 publications

References 48 publications

Mechanobiology of Colorectal Cancer

Mechanobiology of Colorectal Cancer

Application of atomic force microscope in diagnosis of single cancer cells

Attenuation of alcohol‐induced hepatocyte damage by ginsenoside Rg1 evaluated using atomic force microscopy

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