Study of human rotavirus genome by electrophoresis: Attempt of classification among strains isolated in France

The Coexistence of Multiple Bluetongue Virus Electropherotypes in Individual Cattle during Natural Infection

“…Detection by PAGE of extra segments in field isolates of rotaviruses have been described (Lourenco et al, 1981;Sabara et al, 1982;Clarke & McCrae, 1982;Spencer et al, 1983;Rodriguez et al, 1983;Nicolas et al, 1984;Pedley et al, 1984;Pereira et al, 1984); however, this is the first evidence of the co-existence of electrophoretically different BTVs of the same serotype occurring simultaneously during natural infections in the bovine. Similarly, the appearance of extra genome segments or weekly variations in electropherotypes of virus isolated from individual animals (380, 389 and 393) coincided with the natural introduction of a second serotype onto the premises.…”

“…Heterogeneity of genome segments separated by PAGE is common in orbiviruses and in some other Reoviridae (Shatkin et al, 1968;Ramig et al, 1977;Gorman, 1979;Gorman et al, 1977Gorman et al, , 1981Gorman et al, , 1983Hrdy et al, 1979;Walker et al, 1980;Huismans & Bremer, 1981 ;Lourenco et al, 1981 ;Knudson et al, 1982;Sabara et al, 1982;Schroeder et al, 1982;Clarke & McCrae, 1982;Beards, 1982;Gouvea & Schnitzer, 1982;Squire et al, 1983b;Bohl et al, 1984). The effects of this genetic diversity on protein composition, antigenicity and virulence are not known.…”

Rotavirus RNA Segments Sized by Electron Microscopy

“…The RNA can be rapidly extracted and the segments separated by electrophoresis on polyacrylamide gels (Newman et al, 1975;Kalica et al, 1976;Verly & Cohen, 1977;Kalica et al, 1978). Differences in the RNA migration patterns have been found among isolates obtained from different animal species (Kalica et al, 1978;Rodger & Holmes, 1979;Todd & McNulty, 1977;Todd et al, 1980) and the relative mobility of corresponding segments may also differ among isolates obtained from a single species (Verly & Cohen, 1977;Kalica et al, 1978;Rodger & Holmes, 1979;Todd et al, 1980;Espejo et al, 1980;Lourenco et al, 1981 ;'Follett & Desselberger, 1983).…”

Eletroferótipos de rotavírus em crianças com e sem quadros de gastroenterite

“…Lourenço e col. 12 propuseram o agrupamento destes segmentos em 4 aglomerados, compreendendo o aglomerado I os 4 primeiros segmentos, o II, os segmentos 5 e 6, o III, os segmentos 7,8 e 9 e o IV os segmentos 10 e 11. Conhecem-se hoje diferentes perfis eletroferotípicos de rotavírus isolados de espécies animais diferentes, como de cepas de rotavírus isolados da mesma espécie 6,10,12 . O estudo do perfil genômico dos rotavírus permitiu sua divisão em 5 grupos eletroféróticos (A-E), que apresentam como caracterís-tica diferentes associações dos segmentos 7,8 e 9.…”