Study of Histamine Detection using Liquid Chromatography and Gas Chromatography

Munir, Muhammad Abdurrahman; Mackeen, Muhammad Mukram Mohamed; Heng, Lee Yook; Badri, Khairiah Haji

doi:10.32802/asmscj.2021.809

Cited by 14 publications

(14 citation statements)

References 30 publications

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“…Panjang gelombang yang diperoleh di dalam penelitian ini sama dengan panjang gelombang pada penelitian yang dilakukan oleh Khoshneviszadeh et al (2015), penelitian ini mendapati panjang gelombang yang digunakan adalah 293 nm, dan hasil penelitian ini menunjukkan bahwa penggunaan UV sangat tepat karena metodenya yang mudah dan cepat dalam menganalisis senyawa hidrokuinon di dalam suatu sampel. Metode ini telah melakukan metode validasi menurut ICH Q2B Guidelines dan penelitian ini telah memvalidasi linearitas, LOD, LOQ, presisi dan akurasi pada identifikasi senyawa hidrokuinon 12,13 .…”

Section: Gambar 1 Kurva Kalibrasi Hidrokuinonunclassified

Identifikasi Dan Penentuan Hidrokuinon Dalam Beberapa Krim Kosmetik Menggunakan Metode Kromatografi Lapis Tipis Dan Spektrofotometri

Munir

2022

INPHARNMED

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Hidrokuinon merupakan salah satu zat yang biasanya terdapat di dalam krim pemutih yang banyak beredar di pasaran. Hidrokuinon itu sendiri termasuk golongan obat keras yang hanya bisa didapatkan menggunakan resep dokter jika kadarnya lebih dari 2%. Tujuan dari penelitian ini adalah untuk mengetahui kandungan hidrokuinon pada sampel menggunakan metode kromatografi lapis tipis dan spektrofotometri ultraviolet. Spektrofotometri UV-VIS adalah metode pengukuran pada sampel berupa larutan dengan senyawa yang memiliki gugus kromofor, tidak berwarna dan kemurniannya harus tinggi sehingga memenuhi syarat untuk dapat menggunakan metode spektrofotometri UV-VIS. Kromatografi lapis tipis merupakan metode untuk mengetahui ada tidaknya kandungan hidrokuinon dalam krim pemutih dengan melihat dua fase yaitu fase diam dan fase gerak. Berdasarkan hasil grafik maka diperoleh persamaan garis linier yang menghubungkan antara konsentrasi larutan standar dengan absorban y = bx + a, yaitu y = 0,0858 x + 0,1487 dan R 2 = 0,9918. seluruh sampel krim pemutih pada penelitian ini mengandung Hidrokuinon, dan kadar tertinggi hidrokuinon pada sampel diperoleh kadar hidrokuinon sebesar 8.13 ppm.

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Section: Gambar 1 Kurva Kalibrasi Hidrokuinonunclassified

Identifikasi Dan Penentuan Hidrokuinon Dalam Beberapa Krim Kosmetik Menggunakan Metode Kromatografi Lapis Tipis Dan Spektrofotometri

Munir

2022

INPHARNMED

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“…The studies have unveiled numerous techniques for the detection of drugs, whether in single or multicomponents [7][8][9]. Furthermore, only HPLC and UV approaches can be applied for the determination of paracetamol [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

A Modest Uv Spectrophotometric Assisted by Chemometric Approach for Verification of Acetaminophen Level in Various Manufactured Tablets and Syrups in Indonesian Pharmacies

Munir¹,

Inayatullah

Salmani

et al. 2023

Int J App Pharm

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Objective: This study aimed to verify the paracetamol level in some fabricated tablets and syrups in Indonesian pharmacies. Methods: The fabricated tablets and syrups were analyzed using a spectrophotometer UV that was assisted by the chemometric approach. Partial least squares (PLS) and principal component regression (PCR) were the chemometric methods employed to verify the paracetamol level in pharmaceutical products. There were 25 different samples (tablets and syrups) applied in this study. The validation study was employed in this study to verify the approach according to the ICH guidelines. The double-distilled water was applied as a solvent before the samples were analyzed using a spectrophotometer. Results: This technique was efficient and require double-distilled water only as a solvent. The results of this study reveal that there was a deviation in absorbance of the samples with RSD ranging from (0.15-0.45). The technique was linear, ranging from 1.0–6.0 µg·ml-1, with an R2 (0.9991) obtained at 242 nm. The percentage recovery was applied to study the accuracy of the technique and was acquired at 99.18%. The results have shown that the approach was the potential to be applied in estimating the level of paracetamol in tablets and syrups. Conclusion: The detection of paracetamol levels in tablets and syrups using UV spectrophotometric showed satisfactory outcomes. The application of the chemometric approach by using PLC and PCR as the statistical assessment indicated that there was no significant distinction among the validated methods. Furthermore, the method can be used by industries particularly small industries to secure medicines that comply with Indonesian rules.

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“…Aquatic foods have been most related to security issues considering the massive consumption of aquatic food products in Southeast Asia . Physicochemical reactions in food commonly occur during storage.…”

Section: Introductionmentioning

confidence: 99%

Inspecting Histamine Isolated from Fish through a Highly Selective Molecularly Imprinted Electrochemical Sensor Approach

Munir¹,

Rahmawati

Jamal

et al. 2023

ACS Omega

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Numerous analytical approaches have been developed to determine histamine levels in food samples due to its health consequences. Consuming histamine over the Food and Drug Administration (FDA)-regulated 50 mg kg −1 limit would result in chronic toxicity. Consequently, the present study discusses a novel electrochemical approach to evaluate histamine levels in fish products via a molecularly imprinted polymer (MIP) on an electrode surface. The film was produced with electropolymerized polyurethane (PU), which maintained the histamine compound. Fourier-transform infrared (FTIR) spectroscopy was applied to verify the MIP manufactured in this study. The capability of the polymer was measured by assessing its electron shifts with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Differential pulse voltammetry (DPV) was also employed to validate the sensing method. The MIP/ screen-printed electrode (SPE) and non-imprinted polymer (NIP)/SPE recorded a linear response ranging from 1 to 1000 nmol L −1 at the 1.765 and 709 nmol L −1 detection limits. The sensing technique was subsequently utilized to determine the histamine levels in selected samples at room temperature (25 °C). Generally, the sensor allowed the accurate and precise detection of histamine in the fish samples. Furthermore, the approach could be categorized as a simple technique that is low-cost and suitable for on-site detections.

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Study of Histamine Detection using Liquid Chromatography and Gas Chromatography

Cited by 14 publications

References 30 publications

Identifikasi Dan Penentuan Hidrokuinon Dalam Beberapa Krim Kosmetik Menggunakan Metode Kromatografi Lapis Tipis Dan Spektrofotometri

Identifikasi Dan Penentuan Hidrokuinon Dalam Beberapa Krim Kosmetik Menggunakan Metode Kromatografi Lapis Tipis Dan Spektrofotometri

A Modest Uv Spectrophotometric Assisted by Chemometric Approach for Verification of Acetaminophen Level in Various Manufactured Tablets and Syrups in Indonesian Pharmacies

Inspecting Histamine Isolated from Fish through a Highly Selective Molecularly Imprinted Electrochemical Sensor Approach

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