Study of glycated amino acid elimination reaction for an improved enzymatic glycated albumin measurement method

Kouzuma, Takuji; Uemastu, Yumiko; Usami, Tomomi; Imamura, Shigeyuki

doi:10.1016/j.cccn.2004.02.019

Cited by 148 publications

(132 citation statements)

References 11 publications

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“…Results indicated that the GA enzymatic test proved to be reproducible and robust, as reported in previous studies. 5,7 Moreover, the enzymatic method for GA measurement used in the current study showed high accuracy and reproducibility compared with conventional high-performance liquid chromatography methods. 5 Because this method can use an autoanalyzer, rapid and multiple sample determinations can be performed with ease and convenience.…”

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confidence: 75%

Diagnostic Significance of Serum Glycated Albumin in Diabetic Dogs

et al. 2008

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Abstract. Measurements of serum fructosamine, glycated hemoglobin, and glycated albumin (GA) are increasingly used to complement serum glucose concentration for better management of diabetes mellitus. Fructosamine tests are currently not performed in veterinary medicine in Japan. As such, the measurement of GA may serve as a replacement test. Therefore, in the current study, serum GA and fructosamine were evaluated for a positive correlation in dogs, and, depending on the correlation, a reference range of GA percentage would also be determined from healthy control dogs. The degree of glycemic control in diabetic dogs was determined by fructosamine concentration. A positive correlation between GA and fructosamine was observed with both normal and diabetic animals. In addition, the reference interval of serum GA percentage in control dogs was determined to be 11.4-11.9% (95% confidence interval). Interestingly, no significant difference in serum GA percentages was observed between samples from diabetic dogs with excellent glycemic control and control dogs. However, good, fair, and poor glycemic control diabetic dogs resulted in a significant increase in serum GA percentages in comparison with control dogs. These results suggest that serum GA may be a useful diagnostic indicator, substituting for fructosamine, to monitor glycemic control in diabetic dogs.

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confidence: 75%

Diagnostic Significance of Serum Glycated Albumin in Diabetic Dogs

et al. 2008

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“…Serum GA was determined by enzymatic method using albumin-specific protease, ketoamine oxidase and albumin assay reagent (Lucica GA-L; Asahi Kasei Pharma, Tokyo, Japan). 20,21 GA was hydrolyzed to amino acids by an albumin-specific proteinase, and then oxidized by ketoamine oxidase to produce hydrogen peroxide, which was measured quantitatively. Serum GA levels were calculated as the percentage of GA relative to total albumin, which was measured in the same serum sample using a new bromocresol purple method.…”

Section: Discussionmentioning

confidence: 99%

Measurement of glycated hemoglobin and glycated albumin in umbilical cord: evaluation of the glycemic control indicators in neonates

et al. 2010

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Objective: As neonatal blood contains a high proportion of fetal hemoglobin (HbF), it is difficult to use high-performance liquid chromatography (HPLC) method, latex-immunoturbidimetry (LA) method and enzymatic methods, which determine hemoglobin A 1C (HbA 1C ) in order to provide the glycemic control indicators of neonates. In this study, we evaluated glycated hemoglobin (GHb) and glycated albumin (GA) as appropriate indicators of the glycemic control in the neonatal period.Study Design: Umbilical cord blood samples collected during delivery were subjected to measurements of GHb (HPLC methods using two different instruments, LA method, enzymatic method and affinity method) and serum GA.Result: HbA 1C levels determined by the HPLC method, the LA method and the enzymatic method were as low as <3.0% in all the cases. Although GHb determined by the affinity method was 3.6 ± 0.2%, this method may not measure accurately the values of glycated HbF plus glycated HbA. Serum GA was 9.4 ± 1.1%. Conclusion:We speculate that serum GA, but not GHb, could be used as glycemic control indicators in neonates.

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“…GA was measured by an enzymatic method using the Lucica GA-L kit (Asahi Kasei Pharma Corp., Tokyo, Japan) (13). GA was hydrolyzed to amino acids by albumin-specific proteinase and then oxidized by ketoamine oxidase to produce hydrogen peroxide, which was measured quantitatively.…”

Section: Assay Of Ga and Hba 1cmentioning

confidence: 99%

“…Recently, serum glycated albumin (GA) was hypothesized to be an alternative marker for glycemic control in patients with diabetes, which is not affected by changes in the survival time of erythrocytes in the case of type 2 diabetes with hemoglobinopathy (12). Furthermore, the new, improved method, which is free of interference by endogenous glycated amino acids, is unaffected by changes in albumin concentration (13). Therefore, the present study was designed to assess whether the new assay method of GA might provide a better indicator than HbA 1c for glycemic control in HD patients with diabetes.…”

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confidence: 99%

Glycated Albumin Is a Better Glycemic Indicator than Glycated Hemoglobin Values in Hemodialysis Patients with Diabetes

Inaba¹,

Okuno²,

Kumeda³

et al. 2007

Journal of the American Society of Nephrology

469

415

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The significance of glycated albumin (GA), compared with casual plasma glucose (PG) and glycated hemoglobin (HbA 1c ), was evaluated as an indicator of the glycemic control state in hemodialysis (HD) patients with diabetes. The mean PG, GA, and HbA 1c levels were 164.5 ؎ 55.7 mg/dl, 22.5 ؎ 7.5%, and 5.85 ؎ 1.26%, respectively, in HD patients with diabetes (n ‫؍‬ 538), which were increased by 51.5, 31.6, and 17.7%, respectively, compared with HD patients without diabetes (n ‫؍‬ 828). HbA 1c levels were significantly lower than simultaneous PG and GA values in those patients in comparison with the relationship among the three parameters in patients who had diabetes without renal dysfunction (n ‫؍‬ 365), as reflected by the significantly more shallow slope of regression line between HbA 1c and PG or GA. A significant negative correlation was found between GA and serum albumin (r ‫؍‬ ؊0.131, P ‫؍‬ 0.002) in HD patients with diabetes, whereas HbA 1c correlated positively and negatively with hemoglobin (r ‫؍‬ 0.090, P ‫؍‬ 0.036) and weekly dose of erythropoietin injection (r ‫؍‬ ؊0.159, P < 0.001), respectively. Although PG and GA did not differ significantly between HD patients with diabetes and with and without erythropoietin injection, HbA 1c levels were significantly higher in patients without erythropoietin. Categorization of glycemic control into arbitrary quartile by HbA 1c level led to better glycemic control in a significantly higher proportions of HD patients with diabetes than those assessed by GA. Multiple regression analysis demonstrated that the weekly dose of erythropoietin, in addition to PG, emerged as an independent factor associated with HbA 1c in HD patients with diabetes, although PG but not albumin was an independent factor associated with GA. In summary, it is suggested that GA provides a significantly better measure to estimate glycemic control in HD patients with diabetes and that the assessment of glycemic control by HbA 1c in these patients might lead to underestimation likely as a result of the increasing proportion of young erythrocyte by the use of erythropoietin.

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Study of glycated amino acid elimination reaction for an improved enzymatic glycated albumin measurement method

Cited by 148 publications

References 11 publications

Diagnostic Significance of Serum Glycated Albumin in Diabetic Dogs

Diagnostic Significance of Serum Glycated Albumin in Diabetic Dogs

Measurement of glycated hemoglobin and glycated albumin in umbilical cord: evaluation of the glycemic control indicators in neonates

Glycated Albumin Is a Better Glycemic Indicator than Glycated Hemoglobin Values in Hemodialysis Patients with Diabetes

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