Study of folliculogenesis in vivo in guinea pig

Sadeu, Jean Clair; Adriaens, I.; Cortvrindt, R.; Smitz, Johan

doi:10.1016/j.theriogenology.2007.08.028

Cited by 6 publications

(3 citation statements)

References 52 publications

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“…MNF were identified as follicles with normal nuclei and homogeneous cytoplasm surrounded by well-organized granulosa cells. In contrast, follicles were considered degenerated when one or more of the following features were observed: pyknotic oocyte nuclei, an empty space in the oocyte cytoplasm, or granulosa cell degeneration [ 14 ]. Follicle counts were obtained in a high-power field (×400), including 3 developmental stages as primordial follicles, primary follicles and secondary follicles.…”

Section: Methodsmentioning

confidence: 99%

Developmental Status: Impact of Short-Term Ischemia on Follicular Survival of Whole Ovarian Transplantation in a Rabbit Model

Shen¹,

Zhang²,

Chen³

et al. 2015

PLoS ONE

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Ischemia is the first mechanism that provokes the loss of follicles in ovarian grafts over the long term. In whole ovarian transplantation, it remains unknown, however, how changes in follicular development are influenced by short-term ischemia. Fresh whole ovarian orthotopic auto-transplantation was performed in rabbits with 45 min ischemia, and the impact of ischemia on follicular survival and development status was evaluated at different time-points (1 day, 3 days, 1 week, 2 weeks and 1 month). Assessment of follicular quantity and morphology was carried out via histologic analysis. Follicle proliferating status was evidenced by immunostaining with proliferating cell nuclear antigen (PCNA), and the Hedgehog signaling pathway (Patched and Gli); was verified via TUNEL assay. Quantitative PCR was carried out to quantify the mRNA of target genes including PCNA, Patched, Gli, Caspase 3, Bax, and Bcl-2. Compared with its contralateral fresh controls, the morphology, proliferation and apoptosis of the follicles in the grafts showed no significant differences and most primordial follicles were quiescent. However, morphology and proliferation status were significantly decreased 1 week after grafting, in comparison with the longitudinal grafting time. Patched and Gli in the Hedgehog signaling pathway were activated in only the follicles of the grafts. Short-term ischemia slightly impacts follicular survival and development status in whole ovarian grafting. Receiving intervention in the first week post-transplantation might be helpful.

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Section: Methodsmentioning

confidence: 99%

Developmental Status: Impact of Short-Term Ischemia on Follicular Survival of Whole Ovarian Transplantation in a Rabbit Model

Shen¹,

Zhang²,

Chen³

et al. 2015

PLoS ONE

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“…Guinea pigs, unlike other rodents, i.e., rats, mice and hamsters, exhibit follicular formation and development in utero before birth, and thus have a long gestation period of approximately 70 days, similar to that of large animals (Shi et al, 1999;Suzuki et al, 2003;Sadeu et al, 2007). During pregnancy, fetal ovarian development begins with primordial germ cells (PGCs) migrating to and invading embryonic gonads, followed by primordial follicle assembly and development at different time points (Loffler and Koopman, 2002;Suh et al, 2002;Sawyer et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, apoptosis has been implicated in processes associated with normal ovarian development and function, including death of germ and granulosa cells in follicular atresia (Morita and Tilly, 1999;Tilly and Robles, 1999). Several previous studies have investigated morphological changes of ovaries in young and adult guinea pigs (Bookhout, 1945;Sadeu et al, 2007;Wang et al, 2010). However, such knowledge with respect to embryonic guinea pigs is lacking.…”

Section: Introductionmentioning

confidence: 99%

Cell proliferation and apoptosis in the fetal and neonatal ovary of guinea pigs

et al. 2014

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ABSTRACT. The guinea pig is an excellent animal model for studying reproductive biology of adult humans and most domestic animals. Yet, whether this animal might serve as a good model for embryonic stage investigations and determinations of signals affecting or directing ovary development remains unknown. These questions were addressed by examining morphological evolution and the expression of biomarkers of cell proliferation and apoptosis in the ovaries of fetal and neonatal guinea pigs in the present study. Embryonic and neonatal guinea pigs at 30, 40, 50, 60, and 68 days postcoitum (dpc) and at 1 day postpartum (dpp) were evaluated, and the dynamic changes in follicles between 30 dpc and 1 dpp were observed. Results also showed that a critical period of follicular development in guinea pig embryos occurred at 40 to 50 dpc. Moreover, the proliferating-cell nuclear antigen, a cell proliferation marker, immunohistochemically stained healthy follicles, while caspase-3, an apoptosis marker, was mainly observed in atretic follicles. Together, these results demonstrate that cell proliferation and apoptosis contribute to follicular formation, development, and atresia in fetal and neonatal guinea pig ovaries. Furthermore, this study Cell proliferation and apoptosis in the ovary of guinea pigs confirmed that the guinea pig is also an excellent animal model for studying reproductive biology in human and domestic animal embryos.

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Slow-controlled freezing versus speed-cooling for cryopreservation of whole guinea pig ovaries

Wang

et al. 2012

Theriogenology

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Study of folliculogenesis in vivo in guinea pig

Cited by 6 publications

References 52 publications

Developmental Status: Impact of Short-Term Ischemia on Follicular Survival of Whole Ovarian Transplantation in a Rabbit Model

Developmental Status: Impact of Short-Term Ischemia on Follicular Survival of Whole Ovarian Transplantation in a Rabbit Model

Cell proliferation and apoptosis in the fetal and neonatal ovary of guinea pigs

Slow-controlled freezing versus speed-cooling for cryopreservation of whole guinea pig ovaries

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