Slow-controlled freezing versus speed-cooling for cryopreservation of whole guinea pig ovaries

Xu, Zhihui; Wang, Xiaojiao; Wu, Yihao; Meng, Yuanyuan; Wu, Fangfang; Zhou, Na; Chen, Wenwei; Ye, Bilu; Liu, Jun; Zhou, Ying

doi:10.1016/j.theriogenology.2011.08.017

Cited by 8 publications

(6 citation statements)

References 45 publications

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“…Some studies compare Fig. 4 a, b The stromal cell density in different groups vitrification versus slow-cooling by comparing parameters such as follicle viability, histological examination and apoptosis assessment, or hormone concentrations in the culture supernatants, but no consensus has yet been reached [23,24]. Having analyzed the preexperimental data combined in previous studies, we chose slow-cooling in our study, since it enables the precise adjustment of the temperature gradients to achieve an accurate cooling rate through the entire ovary and leads to less ice formation.…”

Section: Discussionmentioning

confidence: 99%

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Lan

Zhao

et al. 2015

J Assist Reprod Genet

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Purpose The study aims to assess the protective effects of dimethyl sulfoxide (DMSO)-free solution based on trehalose on the cryopreservation of a whole sheep ovary and evaluate its use as an efficient cryoprotectant. Method Twenty-one ovaries collected from 6-to 8-month-old non-pregnant female sheep were randomly distributed into three groups, namely, a fresh group, a DMSO-free group, and a DMSO group. The morphology, cell apoptosis (by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyltransferasemediated dUTP nick-end labeling (TUNEL) assay), and mRNA transcript of Bcl-2-associated X protein (BAX) and cold inducible RNA-binding protein (CIRP) (by real-time PCR) of the thawed sheep ovaries and fresh controls were tested to establish a criterion for appraising the results of the cryopreservation.Results (i) The histological assessment indicated that the structure of the DMSO-free ovaries remained largely intact and comparable to those of the fresh control groups; whereas, significant damage was observed in the ovaries of the DMSO group (P<0.05). (ii) The TUNEL assay and mRNA transcript of the BAX assessment showed that the apoptosis parameter in the fresh group was the lowest among all the groups (P<0.05), and the parameter in the DMSO-free group was significantly lower than that in the DMSO group (P<0.05). (iii) The level of the CIRP transcripts increased the most in the DMSO-free group followed by the DMSO group and the fresh control group (P<0.05). Conclusions These results indicate that a DMSO-free cryoprotectant solution, especially a trehalose cryoprotectant, is an efficient cryoprotectant and has a beneficial effect on the cryopreservation of whole sheep ovaries.Capsule These results indicate that a DMSO-free and trehalose-containing cryoprotectant solution has a beneficial effect on the cryopreservation of intact sheep ovaries.Tianqi Du and Lan Chao contributed equally to this work.

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Section: Discussionmentioning

confidence: 99%

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Lan

Zhao

et al. 2015

J Assist Reprod Genet

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“…xeno-or auto-or allografting) include Syrian hamster (Suzuki et al, 2009), agouti (Praxedes et al, 2018), Guinea pig (Xu et al, 2012), cats (many studies, e.g. (Mouttham and Comizzoli, 2016), dogs e.g.…”

Section: Other Speciesmentioning

confidence: 99%

“…Whole sheep ovaries were also slow-frozen using directional solidification (Maffei et al, 2013). Perfusion was also applied in freezing whole ovaries of pig, as mentioned above (Imhof et al, 2004), and in human (Bedaiwy et al, 2006), guinea pig (Xu et al, 2012), and cattle (Isachenko et al, 2013, Westphal et al, 2017. In sheep, live offspring was obtained by Imhof and co-workers (2006) after whole frozen-thawed sheep ovary reanastomosis.…”

Section: Whole Large (Adult) Ovariesmentioning

confidence: 99%

Gene banking and transplantation of (mammalian) ovarian tissue

Woelders¹

2020

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A brief history of cryopreservation and use of ovarian tissue in various taxa 2 Progress in specific (farm) animal species 3 Whole large (adult) ovaries 4 How to improve ovary transplantation in pigs in a future experiment References Centre for Genetic Resources, the Netherlands (CGN) Report | Preface The use of cryopreserved gonadal tissue to reconstitute breeds or breeding lines has been demonstrated in birds (Silversides et al., 2013; Liptoi et al., 2013) and mammals (e.g. Huang et al., 2010) as a highly effective, efficient method. Gonadal transfer is in principle more invasive than some other reproductive technologies, but also has the ethical advantage of reducing numbers of animals needed. Moreover, in some macrolecithal species (birds) there may be no other effective method for ex situ genetic conservation. For bird species, further development and validation of the method and its animal welfare implications are needed, which was successfully undertaken in the EU Horizon 2020 project IMAGE by Liptói and co-workers (2020). For large domestic mammals, such as the pig, proof of principle needs to be demonstrated. We (Egerszegi et al., unpublished) have undertaken a small pilot experiment in which orthotopic homografting of fragments of juvenile pig ovaries into neonate ovariectomized recipient piglets was attempted. This attempt was not successful. The data of the study was obtained at the start of the EU Horizon 2020 project IMAGE and are briefly presented in this report. This report further presents the results of a comprehensive literature review of studies on cryopreservation and transplantation of ovaries or ovarian tissue in various (mammalian) species. The purpose of the review is to describe current possibilities in various mammalian species and to provide suggestions for potential future improvements of methods in pigs or other mammalian farm animal species.

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“…It suggested that controlled freezing (1.2°C/ min) can be an alternative approach for cryopreservation of bovine embryos. The guinea pig has also been known as a useful small animal model to investigate ovarian follicular development and oocyte maturation [78]. Xu et al [78] have showed that whole guinea pig ovaries could be cryopreserved in vitro slow-freezing protocol.…”

Section: Tissue Engineering For Ovary/follicle Cryopreservationmentioning

confidence: 99%

Use of nonhuman primates for the development of bioengineered female reproductive organs

Yun

Kim

Ahn

et al. 2016

Tissue Eng Regen Med

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Nonhuman primates (NHPs) have been widely used in reproductive biology, neuroscience, and drug development since a number of primate species are phylogenetically close to humans. In this review, we summarize the use of NHPs for nonclinical application in the reproductive system disorders including the loss or failure of an organ or tissue. Causes of infertility include congenital aplasia and acquired disorders of the reproductive organs. In addition, anti-cancer treatments can deplete ovarian follicles, leading to premature ovarian failure, infertility and long-term health risks. Along with a limited supply of human reproductive organs, anatomic/physiologic similarities to humans support the need for NHP models (New-World monkeys such as the common marmoset and Old-World monkeys such as cynomolgus and rhesus monkeys) to promote the advances in female infertility studies. For maintaining and executing animal studies using NHP, special protocols including animal care, anesthetic protocol, surgical technique, and immunosuppressive protocol are necessary. With a growing interest in the potential therapies such as endometrial tissue engineering, and ovary/follicle cryopreservation and grafting in Korea, this review can be useful in selecting appropriate animal models and can bridge between nonclinical studies and clinical applications by providing detailed information on the use of NHPs in the field of reproductive organ disorders.

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Slow-controlled freezing versus speed-cooling for cryopreservation of whole guinea pig ovaries

Cited by 8 publications

References 45 publications

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Gene banking and transplantation of (mammalian) ovarian tissue

Use of nonhuman primates for the development of bioengineered female reproductive organs

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