Study of different routes of immunization using outer membrane vesicles of Neisseria meningitidis B and comparison of two adjuvants

Brito, Luciana Tendolini; Rinaldi, F; Gaspar, Emanuelle Baldo; Gonçalves, Caroline Amélia; Portilho, Amanda Izeli; Lima, Gabriela Trzewikoswki de; Gaspari, Elizabeth De

doi:10.1016/j.vaccine.2020.09.081

Cited by 7 publications

(6 citation statements)

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“…As seen in Table 4 , all groups presented intermediate avidity, although OMV+AH avidity was slightly higher. This was found in previous studies [ 24 , 25 ].…”

Section: Discussionsupporting

confidence: 91%

“…While ELISA presents the antigen in the native form, similarly to the bacteria suspension in SBA, the Immunoblotting protocol used denatures the antigens [ 35 ]. In other studies, we verified that OMV+AH supports multiple antigenic recognition [ 24 , 25 ] and, in here, ELISA pointed to increased IgG titers. A further analysis using Empigen BB ® in Immunoblotting to increase the Porin A and Porin B epitopes exposition, could address this issue [ 74 ], or another Immunoblotting could be carried out not using denaturing conditions.…”

Section: Discussionsupporting

confidence: 89%

“…DDA (Sigma-Aldrich, San Louis, MO, USA) was prepared by sonication method in a Sonics Vibra-Cell (Sonics and Materials, Newtown, CT, USA) at 25% amplitude for 20 min, forming bilayer fragments, subsequently centrifuged for 30 min, at 10.000 rpm and 4 °C, to remove ant titanium particles released by the macrotip, and filtered using a 0.45 µm filter membrane [ 21 ]. Both adjuvants, in the described concentrations, have been used before in our laboratory, proving its safety and efficacy with different Neisseria antigens [ 21 , 23 , 24 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

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Immunological Effects of Dimethyldioctadecylammonium Bromide and Saponin as Adjuvants for Outer Membrane Vesicles from Neisseria meningitidis

Portilho

Gaspari

2022

Diseases

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The meningococcal disease is a global health threat, but is preventable through vaccination. Adjuvants improve meningococcal vaccines and are able to trigger different aspects of the immune response. The present work evaluated the immune response of mice against Neisseria meningitidis outer membrane vesicles (OMV) complexed with the adjuvants aluminium hydroxide (AH), via subcutaneous route; and dimethyldioctadecylammonium bromide (DDA) or Saponin (Sap), via intranasal/subcutaneous routes. ELISA demonstrated that all adjuvants increased IgG titers after the booster dose, remaining elevated for 18 months. Additionally, adjuvants increased the avidity of the antibodies and the bactericidal titer: OMVs alone were bactericidal until 1:4 dilution but, when adjuvanted by Alum, DDA or Sap, it increased to 1/32. DDA and Sap increased all IgG isotypes, while AH improved IgG1 and IgG2a levels. Thus, Sap led to the recognition of more proteins in Immunoblot, followed by DDA and AH. Sap and AH induced higher IL-4 and IL-17 release, respectively. The use of adjuvants improved both cellular and humoral immune response, however, each adjuvant contributed to particular parameters. This demonstrates the importance of studying different adjuvant options and their suitability to stimulate different immune mechanisms, modulating the immune response.

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“…As seen in Table 4 , all groups presented intermediate avidity, although OMV+AH avidity was slightly higher. This was found in previous studies [ 24 , 25 ].…”

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Immunological Effects of Dimethyldioctadecylammonium Bromide and Saponin as Adjuvants for Outer Membrane Vesicles from Neisseria meningitidis

Portilho

Gaspari

2022

Diseases

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“…In association to OMVs, Al(OH) 3 was also higher in promoting the generation of IgG avidity antibody, since the number of animals displaying. AI higher than 0.5 was bigger in animals immunized with OMVs/Al(OH) 3 in relation to animals immunize with OMVs/DDA-BF as well as bactericidal activity [ 23 ]. Both adjuvants did not differ in ability to assist the generation of cellular immunity, as evidenced by DTH results.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, DDA-BF was superior to aluminum hydroxide as adjuvant for subcutaneous immunization with OMVs, both with measures of humoral and cellular immunity. In this study, we extend our research analyzing the cellular and humoral immune response promoted by immunization of female individual immune response in mice immunized with OMVs of N. meningitidis B associated with DDA-BF or aluminum hydroxide as adjuvant [ 16 23 24 25 ].…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice

Rinaldi

Gaspar

Brito

et al. 2021

Clin Exp Vaccine Res

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Purpose In the present study, meningococcal serogroup B outer membrane vesicles (OMVs) were associated with bilayer fragments of a cationic lipid, dioctadecyldimethylammonium (DDA-BF), used as adjuvant, in an antigenic preparation tested in adult female outbred mice. This adjuvant was compared to the traditional adjuvant aluminum hydroxide. Materials and Methods The potential in generating humoral response was evaluated by enzyme-linked immunosorbent assay (ELISA). Individual serum was collected and immunoglobulin G (IgG), IgG1, IgG2a, and IgG2b were quantified. Analyses were carried out 15 and 60 days after immunization. Antibodies avidity index were also analyzed by ELISA. Immunoblot and dot-ELISA were carried out to evaluate specific reaction for homologous strains and cross-reactive antigens present in other meningococcal strains isolated in 2011–2012 year, in Brazil. Delayed type hypersensitivity was used as indicative of cellular immunity and compared between two experimental groups, 24 hours after homologous strain challenge. Results The OMVs of Neisseria meningitidis , and N. lactamica (related species) were characterized by electrophoretic separation of proteins in 13% polyacrylamide gel. The strains presented antigens in the range of 8 to 130 kDa, showing a heterogeneous protein migration pattern. In the group immunized with OMVs/DDA-BF, we found no significant production of total IgG 15 days after the first immunization. On the other hand, 60 days after first immunization both adjuvants act benefiting total IgG production similarly. The antibodies of the IgG isotype produced by animals immunized after one or two doses after first immunization, showed intermediate and high avidity, independent on the adjuvant used. In both experimental groups the swelling of the footpads was significantly higher than those of the controls, suggesting that only one dose was enough to stimulate the generation of cellular immunity. Conclusion The use of this cationic adjuvant for N. meningitidis OMVs preparation revealed good potential for future new antigen preparation for N. meningitidis vaccine.

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Engineered bacterial extracellular vesicles for osteoporosis therapy

Liu

Zhang

et al. 2022

Chemical Engineering Journal

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Study of different routes of immunization using outer membrane vesicles of Neisseria meningitidis B and comparison of two adjuvants

Cited by 7 publications

References 59 publications

Immunological Effects of Dimethyldioctadecylammonium Bromide and Saponin as Adjuvants for Outer Membrane Vesicles from Neisseria meningitidis

Immunological Effects of Dimethyldioctadecylammonium Bromide and Saponin as Adjuvants for Outer Membrane Vesicles from Neisseria meningitidis

Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice

Engineered bacterial extracellular vesicles for osteoporosis therapy

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