Study of correlation between state and composition of lipid phase and change in erythrocytes structure under induction of oxidative processes

Abstract

We investigated human blood erythrocytes under oxidative stress in vitro and established a correlation between composition and state of lipids and changes in erythrocytes structure under induced oxidative stress. These changes may serve as an indicator of not only the erythrocyte state but of systemic processes that occur at the level of the whole organism, including various pathologies as well. We found that a pyrimidine derivative xymedon used in the present study is an effective inhibitor of oxidative processes. Xymedon may be useful as an antioxidant for preserving the structural and functional characteristics of the erythrocytes in the treatment of organisms exposed to physical and toxic factors causing oxidative stress.

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

“…Changes in the phospholipid and fatty-acid composition of the erythrocyte lipids indicate structural rearrangements in the membrane of erythrocytes and thus, point to the functional changes affecting the erythrocytes in general, as well as the primary oxygen-transport protein of erythrocytes, Hb (membrane-bound and cytoplasmic form) [6,11]. In addition, changes in the functional and morphological properties of Hb and erythrocytes in general can throw light on the degree and the nature of the pathological process accompanied by hypoxia.…”

“…Changes in the lipid and fatty-acid composition as well as in the viscosity of the membrane leads to changes in the erythrocyte size and shape [11]. This, in turn, can indirectly affect the conformation and the oxygen-binding properties of membrane-bound and total Hb contained in the erythrocyte.…”

“…The study of lipid composition and state will help to understand their involvement in the functioning of erythrocytes and, in particular, in the regulation of the oxygen-transport capacity of Hb under hypoxia. The state of the erythrocyte membranes has bearing on their morphological characteristics (surface area, diameter, volume) and on the intracellular distribution of Hb [11]. The changed morphometric parameters of erythrocytes entails, in turn, changes in Hb conformation in general and in particular its haematoporphyrin (HP) and, as a consequence, a change in the oxygen-binding and oxygen-transport abilities of erythrocyte Hb.…”

Study of Morphological Characteristics of Pigeon?s Erythrocytes and Hemoglobin Properties under the Influence of Ions Ca2+

“…Fluorescence spectra were recorded using a spectrofluorimeter RF-5301РС (Shimadzu, Japan) with a xenon lamp with a power 150 W. Morphological changes in erythrocytes were studied with the help of the method of LIM on an automated interference microscope MII-4M (RUSSIA). Using this method we can research the dynamics of changes in cell shape and structure, as well as functional condition [11,12]. In this work was used the laser with wavelength 650 Nm, the lens with magnification х32 and a numerical aperture 0.65.…”

The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

“…Many studies [1][2][3], including ours [4][5][6], have provided evidence that the lipid phase plays an active role in the regulation of the oxygen-transport function of haemoglobin during hypoxia, which is the cause and consequence of many cardiovascular diseases [7]. However, the human body often faces a situation when in some tissues and, in particular, in the blood, there may be an excessive amount of oxygen.…”