Study of Bone Marrow and Embryonic Stem Cell-Derived Human Mesenchymal Stem Cells for Treatment of<i>Escherichia coli</i>Endotoxin-Induced Acute Lung Injury in Mice

Qin, Hao; Zhu, Yinggang; Monsel, Antoine; Gennai, Stéphane; Lee, Travis; Xu, Fengyun; Lee, Jae Woo

doi:10.5966/sctm.2015-0006

Cited by 64 publications

(57 citation statements)

References 49 publications

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“…Exosomes (microvesicles) secreted from MSCs may play a pivotal bi-directional role in communication between stem cell and injured cell. Consequently, the stem cell phenotype can be induced to facilitate self-repair or to acquire specific features of the tissue [Hao et al, 2015]. Such microvesicles derived from BM-MSCs have been shown to exert a protective role in acute pancreatitis induced by sodium taurocholate [Yin et al, 2016] and preserve insulin-producing cells in the islets of mice with type I DM [Shigemoto-Kuroda et al, 2017].…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate the Pancreatic Changes of Chemically Induced Hypothyroidism by Carbimazole in Male Rats

Arafa

Gouda

El-naseery

et al. 2018

Cells Tissues Organs

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We induced hypothyroidism (HT) in male rats through chronic oral administration of carbimazole and then tested whether an i.v. injection of rat bone marrow-derived mesenchymal stem cells (BM-MSCs) could ameliorate the HT-induced changes in pancreatic structure and function. The thyroid and pancreatic function tests, as well as total antioxidant capacity (TAC) and malondialdehyde (MDA) were estimated. The pancreatic structure was evaluated by hematoxylin and eosin (H&E) stain. Insulin protein and cleaved caspase-3 were detected immunohistochemically. The degree of apoptosis was assessed by TUNEL assay. The morphometric measurements were done by an image analyzer system and the obtained data were statistically analyzed. HT rats showed hyperglycemia associated with insulin deficiency, decreased TAC and increased MDA levels. H&E-stained sections showed that the pancreatic septa were infiltrated with acidophilic material. Some acini were vacuolated while others showed depleted acidophilia and dilated lumina. Spindle-shaped cells were accumulated within deformed islets in HT rats. The positive reaction with anti-cleaved caspase-3 was exclusively noted in the cytoplasm of islet cells with no immunostaining reaction in the acinar and ductal cells, whereas the positively stained nuclei with TUNEL were demonstrated in the islet and acinar cells. A significant increase in the apoptotic index % of both markers was detected. Injection of BM-MSCs in HT rats restored all biochemical indicators of disturbed pancreatic function to normal level and improved pancreatic structure, resulting in a clear septa and normal appearance of acini and islets. In conclusion, many of the significant structural and func tional pancreatic alterations detected in HT rats were ameliorated after the injection of BM-MSCs. These data demonstrate the ability of BM-MSCs to repair pancreatic disturbances. Further studies on humans are necessary to determine the potential clinical applications of BM-MSCs.

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Section: Discussionmentioning

confidence: 99%

Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate the Pancreatic Changes of Chemically Induced Hypothyroidism by Carbimazole in Male Rats

Arafa

Gouda

El-naseery

et al. 2018

Cells Tissues Organs

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“…Interestingly, hESC-derived MSCs performed more effectively compared to bone marrow MSCs in reducing experimental autoimmune encephalitis [97], pulmonary hypertension [98], and acute pulmonary injury [99], and hiPSC-derived MSCs outperformed bone marrow MSCs in a mouse model of anthracycline-induced cardiomyopathy [100]. MB-derived MSCs have already been tested mouse models of hindlimb ischemia and chronic airway disease.…”

Section: Mb Potential For Cellular Therapiesmentioning

confidence: 99%

The mesenchymoangioblast, mesodermal precursor for mesenchymal and endothelial cells

Slukvin

Kumar

2018

Cell. Mol. Life Sci.

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Mesenchymoangioblast (MB) is the earliest precursor for endothelial and mesenchymal cells originating from APLNRPDGFRαKDR mesoderm in human pluripotent stem cell cultures. MBs are identified based on their capacity to form FGF2-dependent compact spheroid colonies in a serum-free semisolid medium. MBs colonies are composed of PDGFRβCD271EMCNDLK1CD73 primitive mesenchymal cells which are generated through endothelial/angioblastic intermediates (cores) formed during first 3-4 days of clonogenic cultures. MB-derived primitive mesenchymal cells have potential to differentiate into mesenchymal stromal/stem cells (MSCs), pericytes, and smooth muscle cells. In this review, we summarize the specification and developmental potential of MBs, emphasize features that distinguish MBs from other mesenchymal progenitors described in the literature and discuss the value of these findings for identifying molecular pathways leading to MSC and vasculogenic cell specification, and developing cellular therapies using MB-derived progeny.

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“…Administration of BM‐MSCs significantly reduced the influx of white blood cells, neutrophils and decreasing the secretion of the inflammatory cytokines, macrophage inflammatory protein‐2, and tumor necrosis factor‐α, in the injured alveolus. BM‐MSCs also reduced extravascular lung water and the total protein levels …”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells therapeutic potential alleviate lipopolysaccharide‐induced acute lung injury in rat model

Soliman

Mansour

Hassan

et al. 2018

J Biochem & Molecular Tox

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Inhalation of bacterial endotoxin induces an acute inflammation in the lower respiratory tract. The current study examined the therapeutic effects of bone marrow mesenchymal stem cells (BM-MSCs) in lipopolysaccharide (LPS)-induced pulmonary congestion in rats as compared with dexamethasone (Dexa) and sodium bicarbonate (NaHCO ). LPS (20 µL of LPS of Escherichia coli in each nostril for two consecutive days) induced lung injury as marked by an elevation of number of inflammatory cells especially neutrophils, increased total protein levels, elevation of lipid peroxidation, and reduction of reduced glutathione in bronchoalveolar lavage along with the reduction of reduced glutathione. These deleterious effects were hampered after treatment with BM-MSCs (1 × 10 cells/rat) once before acute lung injury (ALI) induction with LPS to an even better extent than Dexa (2 mg/kg once, ip) and NaHCO (10-15 mL/day for two consecutive days). In summary, BM-MSCs have the ability to suppress the endotoxin-induced systemic inflammatory response and could prove to be a novel approach to therapy for ALI in rats.

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Study of Bone Marrow and Embryonic Stem Cell-Derived Human Mesenchymal Stem Cells for Treatment ofEscherichia coliEndotoxin-Induced Acute Lung Injury in Mice

Cited by 64 publications

References 49 publications

Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate the Pancreatic Changes of Chemically Induced Hypothyroidism by Carbimazole in Male Rats

Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate the Pancreatic Changes of Chemically Induced Hypothyroidism by Carbimazole in Male Rats

The mesenchymoangioblast, mesodermal precursor for mesenchymal and endothelial cells

Mesenchymal stem cells therapeutic potential alleviate lipopolysaccharide‐induced acute lung injury in rat model

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