Study of biological effects and oxidative stress related responses in gamma irradiatedArabidopsis thalianaplants

Abstract

Abstract. This study aimed at investigating biological effects in Arabidopsis thaliana leaves and roots irradiated for 72 h with 3.5 Gy or 30 Gy of gamma radiation, and to unravel oxidative stress related responses to achieve a better understanding of the importance of the cellular redox balance as a modulator in gamma radiation stress. A. thaliana performs like a rather radioresistant plant species as no alterations on growth and only minor alterations in the nutrient profile were observed. Gamma irradiation did not seem to induce an NADPH mediated oxidative burst and lipid peroxidation appeared to be directly induced by ionizing radiation rather than mediated through LOX activity. As ionizing radiation can cause indirect damage via water radiolysis, H 2 O 2 is hypothesized to be an important reactive oxygen species under radiation stress. Although most H 2 O 2 -scavenging enzymes remained unchanged, important alterations were observed for CAT1, CAT2 and CAT3 expression.

Impact of Proton Beam Irradiation on the Growth and Biochemical Indexes of Barley (Hordeum vulgare L.) Seedlings Grown under Salt Stress

“…The generation of reactive oxygen species (ROS) can be induced by diverse abiotic stresses, such as salt, drought, heat, or heavy metal stress. ROS is a collective term used both for free radicals, like hydroxyl radical (•OH), superoxide anion (•O 2 − ), and alkoxy radicals (RO•) as well as non-radical (molecular) forms such as hydrogen peroxide (H 2 O 2 ) and singlet oxygen ( 1 O 2 ) [ 58 , 59 ]. Salinity-mediated hyperosmotic and hyperionic stress causes supplementary stress in plants, well-known as oxidative stress.…”