Study of baicalin on sympathoexcitation induced by myocardial ischemia via P2X3 receptor in superior cervical ganglia

Zhang, Jun; Liu, Shuangmei; Xu, Biao; Li, Guodong; Huang, An; Wu, Bing; Peng, Li; Song, Miaomiao; Xie, Qiuyu; Lin, Weijian; Xie, Wei; Wen, Shiyao; Zhang, Zhedong; Xu, Xiaoling; Liang, Shangdong

doi:10.1016/j.autneu.2014.12.001

Cited by 17 publications

(28 citation statements)

References 42 publications

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“…NONRATT021972 siRNA decreased the BzATP-induced currents in HEK293 cells expressing the hP2X 7 receptor by an increase in blood pressure and sympathetic nerve activity [14,19,24,29]. Previous data from our laboratory showed that the serum ATP concentration was increased after the MI injury [32]. Moreover, we also observed that the SBP, DBP, and HR in the MI group were increased compared to the control and sham groups.…”

Section: Discussionsupporting

confidence: 68%

“…The rats were randomly divided into seven groups (with eight rats in each group): control group (con); sham group; myocardial ischemic group (MI); myocardial ischemic rats treated with the P2X 7 receptor antagonist brilliant blue G (BBG) group (MI + BBG); myocardial ischemic rats treated with the NONRATT021972 siRNA group (MI + NONRATT021972 si); myocardial ischemic rats treated with the P2X 7 siRNA group (MI + P2X 7 si) and myocardial ischemic rats treated with the scrambled siRNA group (MI + SC si). The myocardial ischemia model was established by occlusion of the left coronary artery (LCA) [32]. Briefly, the rats were anesthetized with 10 % chloral hydrate (0.3 ml/100 g body weight) via intraperitoneal injection.…”

Section: Animals and Myocardial Ischemia Modelsmentioning

confidence: 99%

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Long noncoding NONRATT021972 siRNA normalized abnormal sympathetic activity mediated by the upregulation of P2X7 receptor in superior cervical ganglia after myocardial ischemia

Zou

Liu

et al. 2016

Purinergic Signalling

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Previous studies showed that the upregulation of the P2X 7 receptor in cervical sympathetic ganglia was involved in myocardial ischemic (MI) injury. The dysregulated expression of long noncoding RNAs (lncRNAs) participates in the onset and progression of many pathological conditions. The aim of this study was to investigate the effects of a small interfering RNA (siRNA) against the NONRATT021972 lncRNA on the abnormal changes of cardiac function mediated by the up-regulation of the P2X 7 receptor in the superior cervical ganglia (SCG) after myocardial ischemia. When the MI rats were treated with NONRATT021972 siRNA, their increased systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), low-frequency (LF) power, and LF/HF ratio were reduced to normal levels. However, the decreased high-frequency (HF) power was increased. GAP43 and tyrosine hydroxylase (TH) are markers of nerve sprouting and sympathetic nerve fibers, respectively. We found that the TH/GAP43 value was significantly increased in the MI group. However, it was reduced after the MI rats were treated with NONRATT021972 siRNA. The serum norepinephrine (NE) and epinephrine (EPI) concentrations were decreased in the MI rats that were treated with NONRATT021972 siRNA. Meanwhile, the increased P2X 7 mRNA and protein levels and the increased p-ERK1/2 expression in the SCG were also reduced. NONRATT021972 siRNA treatment inhibited the P2X 7 agonist BzATPactivated currents in HEK293 cells transfected with pEGFP-P2X 7 . Our findings suggest that NONRATT021972 siRNA could decrease the upregulation of the P2X 7 receptor and improve the abnormal changes in cardiac function after myocardial ischemia.

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Section: Discussionsupporting

confidence: 68%

Section: Animals and Myocardial Ischemia Modelsmentioning

confidence: 99%

Long noncoding NONRATT021972 siRNA normalized abnormal sympathetic activity mediated by the upregulation of P2X7 receptor in superior cervical ganglia after myocardial ischemia

Zou

Liu

et al. 2016

Purinergic Signalling

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“…Ligation of the left anterior descending coronary artery causes myocardial hypoxia and induces myocardial ischemic injury. Myocardial ischemic damage increases myocardial cell permeability, which results in elevated myocardial enzymes in peripheral blood [18,23,37]. Myocardial enzymes, such as CK-MB, CK, LDH, and AST, are myocardial injury markers [18,23,37].…”

Section: Discussionmentioning

confidence: 99%

“…Rats were randomly divided into seven groups (eight rats per group): control group (Con group), sham group, myocardial ischemic group (MI group), myocardial ischemic rats treated with the P2X 7 receptor antagonist brilliant blue G (BBG) group (MI + BBG group), myocardial ischemic group rats treated with NONRATT021972 siRNA group (MI + NONRATT021972 si), myocardial ischemic rats treated with P2X 7 siRNA group (MI + P2X 7 si), and myocardial ischemic rats transfected with scrambled siRAN group (MI + SC si group). The myocardial ischemic model was established via occlusion of the left coronary artery (LCA) [18]. Briefly, rats were anesthetized with 10 % chloral hydrate (0.3 mL/100 g body weight) via intraperitoneal injection.…”

Section: Methodsmentioning

confidence: 99%

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LncRNA NONRATT021972 involved the pathophysiologic processes mediated by P2X7 receptors in stellate ganglia after myocardial ischemic injury

Zou

Xie

et al. 2015

Purinergic Signalling

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Adenosine triphosphate (ATP) acts on P2X receptors to initiate signal transmission. P2X 7 receptors play a role in the pathophysiological process of myocardial ischemic injury. Long noncoding RNAs (lncRNAs) participate in numerous biological functions independent of protein translation. LncRNAs are implicated in nervous system diseases. This study investigated the effects of NONRATT021972 small interference RNA (siRNA) on the pathophysiologic processes mediated by P2X 7 receptors in stellate ganglia (SG) after myocardial ischemic injury. Our results demonstrated that the expression of NONRATT021972 in SG was significantly higher in the myocardial ischemic (MI) group than in the control group. Treatment of MI rats with NONRATT021972 siRNA, the P2X 7 antagonist brilliant blue G (BBG), or P2X 7 siRNA improved the histology of injured ischemic cardiac tissues and decreased the elevated concentrations of serum myocardial enzymes, creatine kinase (CK), CK isoform MB (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) compared to the MI rats. NONRATT021972 siRNA, BBG, or P2X 7 siRNA treatment in MI rats decreased the expression levels of P2X 7 immunoreactivity, P2X 7 messenger RNA (mRNA), and P2X 7 protein, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and phosphorylated p38 mitogenactivated protein kinase (p38 MAPK) in the SG compared to MI rats. NONRATT021972 siRNA treatment prevented the pathophysiologic processes mediated by P2X 7 receptors in the SG after myocardial ischemic injury.

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P2X receptors mediated abnormal interaction between satellite glial cells and neurons in visceral pathological changes

Wang

Yang

et al. 2019

Cell Biology International

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The adenosine triphosphate (ATP)-gated P2X receptor cation channel family consists of permeable ligand-gated ion channels that expand on the binding of extracellular adenosine 5'-ATP. ATP-gated P2X receptors are trimer ion channels that assemble homo or isomer from seven cloned subunits. P2X receptors are discovered mostly in mammalian and are being found in an increasing number of non-vertebrates, such as zebrafish, bullfrog, and ameba. P2X receptors are involved in many physiological processes, including regulation of heart rhythm and contractility, and regulation of pain, especially chronic pain and glia integration. This review summarizes the current studies on the regulation of P2X receptors in abnormal neuronal-glial interaction and the pathological changes in viscera, especially in myocardial ischemia.

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Study of baicalin on sympathoexcitation induced by myocardial ischemia via P2X3 receptor in superior cervical ganglia

Cited by 17 publications

References 42 publications

Long noncoding NONRATT021972 siRNA normalized abnormal sympathetic activity mediated by the upregulation of P2X7 receptor in superior cervical ganglia after myocardial ischemia

Long noncoding NONRATT021972 siRNA normalized abnormal sympathetic activity mediated by the upregulation of P2X7 receptor in superior cervical ganglia after myocardial ischemia

LncRNA NONRATT021972 involved the pathophysiologic processes mediated by P2X7 receptors in stellate ganglia after myocardial ischemic injury

P2X receptors mediated abnormal interaction between satellite glial cells and neurons in visceral pathological changes

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