Study of Bacterial Pathogens in Urinary Tract Infection and their Antimicrobial Sensitivity Pattern

Abstract

Background: Urinary tract infection (UTI) is one of the most common bacterial infections in Bangladesh. Antimicrobial agents are used for its treatment. The increase in antibiotic resistance among uropathogens is a global problem.Objective: This study was designed to find out the prevalence of UTI, its causative agents and their antimicrobial susceptibility patterns among suspected patients of UTI attending Dhaka Dental College Hospital, Dhaka.Methodology: This cross-sectional study was carried out in Dhaka Dental College Hospital, Dhaka during the period of November 2014 to May 2017. Clinically diagnosed cases of UTI irrespective of age and sex from out-patient department and in-patient department were selected for the purpose of the study. For culture, the urine samples were inoculated on 5% sheep blood agar and MacConkey’s agar media using calibrated loop following standard bacteriological technique. After the incubation period, the plate were examined for bacterial pathogen. The disk diffusion method (Kirby Bauer’s) was used to determine the antimicrobial susceptibility of isolates. Standard inoculums were inoculated on Mueller-Hinton agar and incubated at 37oC for 24 hours.Result: Ninety three urine samples were studied. Among the 93 samples most of them were in the age group of 21 to 30 years. Out of 93 samples, 26 (27.95%) samples were found culture positive. Escherichia coli (92.30%) was found to be the predominant organism. Regarding antimicrobial sensitivity pattern Esch. coli was found to be most sensitive to Imipenem (100%), Amikacin (87.5%), and Nitrofurantoin (83.33%).Conclusion: In conclusion young aged female are commonly suffering from UTI and Escherichia coli is the most common isolated bacteria.Bangladesh Journal of Infectious Diseases, December 2017;4(2):40-44

Prevalence of urinary tract infections, associated risk factors, and antibiotic resistance pattern of uropathogens in young women at Noakhali, Bangladesh

“…Our study revealed that E. coli (32.08%) was the most dominant UTI-causing bacteria and this finding is comparable with a previous study (31.5%) in India (Subramanian et al, 2011). However, The present report does not agree with the results of other studies in Bangladesh where relatively higher rates of E. coli (59.30%-92.30%) has been documented (Haque et al, 2015;Parveen and Rahim, 2017;Rahman et al, 2009;Rahman et al, 2014;Yasmeen et al, 2015). The second most frequent uropathogen in our study was Staphylococcus saprophyticus (24.53%).…”

“…It is on record that lower rates of 9% and 16.4% in Bangladesh and 12.7% in India, have been reported by some authors (Ahmed et al, 2008;Begum et al, 2006, Rahman et al, 2014. Other previous authors recorded higher UTI prevalence rates of 32.25%, 48.5%, and 66.15% in Bangladesh (Hossain et al, 2017;Moue et al, 2015;Parveen and Rahim, 2017). The high or low UTI incidence rates may be Frequency (%) attributed due to the subjects residing environmental condition.…”

“…The peak prevalence of UTI among teenagers is around 20% (Amali et al, 2009), 25 to 30% incidence in females aged 20-40 years and ranges 4% to 43% in older females over 60 years of age (Mittal et al, 2009). Previous studies in Bangladesh revealed that the prevalence of UTI in young females as 48.5% (21-30 years ages) in the outpatient department in a hospital (Moue et al, 2015) , 32.25% (21-30 years ages) of patients attending Dhaka Dental College Hospital (Parveen and Rahim, 2017), 66.15% (15-32 years ages) in the northern part of the country (Hossain et al, 2017), and 77.78% (20-29 years ages) in garments worker (Begum et al, 2006). UTI is predominantly caused by Gram-negative bacteria like Escherichia coli, Proteus spp., Klebsiella spp., Pseudomonas aeruginosa, Acinetobacter, Serratia and Morganella margani.…”

Antimicrobial Sensitivity Pattern of Escherichia Coli Isolates From Various Clinical Samples in a Tertiary Care Hospital

“…E Coli is the most commonly encountered human pathogen which has been associated with various clinical manifestations like UTI, diarrhea, abdominal infections, pneumonia, meningitis, wound and soft tissue infections, bacteremia etc 1 There are reports from different regions of India and other countries on antimicrobial sensitivity pattern of E coli isolated from various clinical samples like urine 2,3,4,5 , skin and soft tissue infections 6 , stool 7,8 , sputum 9. blood 10 . The prevalence of ESBL producing E coli is increasing and resistance to E coli varies in different geographical regions 11 .Analyzing resistance pattern of Escherichia coli to commonly prescribed antibiotics in the clinical practice is essential and helpful in improving the efficacy of empirical treatment.…”

Bacterial association in urinary tract infection and their drug resistance among patients in Rajshahi, Bangladesh

“…Women are more affected by UTI than men because of their physical structure though men over the age of 60 years with prostatic hypertrophy are at a greater risk. 4 Some factors such as gender, age, race, circumcision, human immunodeficiency virus (HIV), diabetes, urinary catheter, genitourinary tract abnormalities, and pregnancy are the major risk factors for UTIs. 1,[5][6][7][8][9][10][11][12][13] UTIs can be community-acquired or nosocomial.…”