Study of antioxidant effects on malignant glioma cells by constructing a tumor-microvascular structure on microchip

Liu, Hanyang; Jie, Mingsha; He, Ziyi; Li, Haifang; Lin, Jin‐Ming

doi:10.1016/j.aca.2017.05.009

Cited by 29 publications

(40 citation statements)

References 48 publications

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“…Functional hydrogels can mimic native ECM with tunable chemical compositions and mechanical properties, which are conductive to replicate the complex microenvironment of human organs. Hydrogels acted as 3D matrices or scaffolds have been incorporated into OOC to engineer human tissues with multicellular architecture and organ‐specific functions by spatial control of microenvironment cues . A variety of 3D parenchymal tissues in hydrogel‐based organs‐on‐chips have been reported, such as liver, heart, skeletal muscle, intestine, and nasal mucosa …”

Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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The major motivation is to better mimic human physiology and functions at multiscales from the molecular to the cellular, tissue, organ or even whole organism level. Current model systems primarily rely on the monolayer cell cultures and animal models. Simplistic monolayer cultures have their advantages, but they are often significantly different in gene expression, epigenetics, and cell function compared to native 3D tissues. [1,2] Also, they often lack cell-cell and cell-matrix interactions, [2,3] leading to the absence of tissue specific properties. Although animal models are widely used in biomedical research, they fail to faithfully predict human responses in a physiologically relevant manner due to the significant species divergences. [4] The limitations of these systems have provided an impetus for the development of alternative cell-based 3D models in vitro that better resemble the complex functionalities of living organs. Over the last several years, organoids and organ-on-a-chip (OOC), representing the major technological breakthrough, have emerged as two distinct model systems to achieve the same goal of building 3D organotypic models in vitro by bridging the gap between animal models and monolayer cultures. These engineered models are different in terms of cell source, tissue composition, architectural variability, functional features, scales, cellular fidelity, etc. Organoids, primarily evolving from developmental principles, refer to 3D multicellular tissues by self-organization of stem cells or organ-specific progenitors, which can recapitulate the intricate architectures and functionalities of in vivo organs. [5][6][7] Recent breakthroughs in organoid technology have enabled the successful generation of a variety of human organoids, such as the brain, [8,9] intestine, [10,11] liver, [12] kidney, [13,14] lung, [15] etc. These near physiological 3D organoids have garnered momentum for their potential applications in human organ development and disease modeling, drug screening and regenerative medicine. The explosion of organoids research has been catalyzed by the significant progress of stem cell biology and availability of human stem cells. In contrast, the OOC, relying on the bioengineering design principle, is a miniaturized in vitro model that can recreate the functional units of living organs on a microfluidic cell culture device using predifferentiated cells, often cell lines. [1,16] The OOC is primarily evolved from the convergence of tissue engineering and microfabricated technologies, which is characterized by the capability to simulate cellular microenvironment by precise control over fluid flow, mechanical forces and biochemical factors. [4,17] Remarkable progress has been made Significant advances in materials, microscale technology, and stem cell biology have enabled the construction of 3D tissues and organs, which will ultimately lead to more effective diagnostics and therapy. Organoids and organs-on-a-chip (OOC), evolved from developmental biology and bioengineering principles, have e...

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Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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“…Redox-active compounds such as MnTE-2-PyP and hydroxynaphthoquinones can downregulate p300 activity [108–111]. The use of alpha-lipoic acid, a dietary antioxidant supplement, has been shown to protect against RIF in mice by downregulating expression and activity of p300/CBP [112–115]. Similarly, inhibition of p300 activity using curcumin also reduces cardiac fibrosis and hypertrophy [32, 94, 116].…”

Section: Ros-mediated Histone Modification Changes In Rifmentioning

confidence: 99%

Reactive Oxygen Species Drive Epigenetic Changes in Radiation-Induced Fibrosis

Shrishrimal

Kosmacek

Oberley‐Deegan

2019

Oxidative Medicine and Cellular Longevity

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Radiation-induced fibrosis (RIF) develops months to years after initial radiation exposure. RIF occurs when normal fibroblasts differentiate into myofibroblasts and lay down aberrant amounts of extracellular matrix proteins. One of the main drivers for developing RIF is reactive oxygen species (ROS) generated immediately after radiation exposure. Generation of ROS is known to induce epigenetic changes and cause differentiation of fibroblasts to myofibroblasts. Several antioxidant compounds have been shown to prevent radiation-induced epigenetic changes and the development of RIF. Therefore, reviewing the ROS-linked epigenetic changes in irradiated fibroblast cells is essential to understand the development and prevention of RIF.

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“…However, when addressing different biomarkers, this is often not the case and new components need to be developed to achieve detection. Liu et al reported a 3D co-culture of human umbilical vein endothelial cells (HUVEC) and human glioblastoma (U87) cells in a microfluidic device to study the antioxidant effects of α-lipoic acid, catechin, and ascorbic acid by monitoring the intracellular ROS and glutathione levels [44]. The reason behind targeting ROS is that increased ROS promotes the tumour malignancy by triggering secretion of vascular endothelial growth factor (VEGF), which stimulates the vascularisation of the tumour.…”

Section: Optical Methodsmentioning

confidence: 99%

Recent Progress in Lab-On-a-Chip Systems for the Monitoring of Metabolites for Mammalian and Microbial Cell Research

Dervisevic

Tuck

Voelcker

et al. 2019

Sensors

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Lab-on-a-chip sensing technologies have changed how cell biology research is conducted. This review summarises the progress in the lab-on-a-chip devices implemented for the detection of cellular metabolites. The review is divided into two subsections according to the methods used for the metabolite detection. Each section includes a table which summarises the relevant literature and also elaborates the advantages of, and the challenges faced with that particular method. The review continues with a section discussing the achievements attained due to using lab-on-a-chip devices within the specific context. Finally, a concluding section summarises what is to be resolved and discusses the future perspectives.

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Study of antioxidant effects on malignant glioma cells by constructing a tumor-microvascular structure on microchip

Cited by 29 publications

References 48 publications

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Reactive Oxygen Species Drive Epigenetic Changes in Radiation-Induced Fibrosis

Recent Progress in Lab-On-a-Chip Systems for the Monitoring of Metabolites for Mammalian and Microbial Cell Research

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