Study of antioxidant activity of pyrimidinium betaines by DPPH radical scavenging method

Fatiha, Malki; Touati, Abdelkader

doi:10.15406/japlr.2019.08.00308

Cited by 20 publications

(9 citation statements)

References 17 publications

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“…DPPH has been widely adopted in measuring the free radical scavenging activity of antioxidants. This assay is solely based on the reduction of an alcoholic DPPH solution using a hydrogen-donating antioxidant (Fatiha and Abdelkader, 2019).…”

Section: Discussionmentioning

confidence: 99%

Free Radical Scavenging and In vitro Cytotoxic Activity of Bugnay (Antidesma bunius) Leaves Extract against A549 Human Lung Adenocarcinoma and HCT-116 Human Colorectal Cancer Cell Lines

Geronimo

Bancual

et al. 2020

Indones J Cancer Chemoprevent

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Cancer is one of the significant causes of mortality worldwide. Studies on antineoplastic drugs focused on natural products have revealed several mechanisms to inhibit cancer cells. Bugnay (Antidesma bunius) leaves showed potentials due to its activity observed against brine shrimp and breast cancer cells. However, there is still limited knowledge about its activity against other human cancer cells. This study focused on determining the phytochemical compounds in A. bunius leaves extract, the free radical scavenging activity of the extract using the Diphenylpicrylhydrazyl (DPPH) method, and in vitro cytotoxic activity against two cancer cell lines, namely HCT-116 human colorectal and A549 human lung adenocarcinoma cancer cell lines by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemicals identified were unsaturated lactones, flavonoids, phenolics, diterpenes, saponins, tannins, carbohydrates, and reducing sugars. The extract showed significant free radical scavenging activity and a direct correlation of activity with concentration levels. It also exhibited cytotoxic activity against HCT-116 human colorectal and A549 human lung adenocarcinoma. Hence, A. bunius leaves have the potential to be a source of antioxidant and antineoplastic compounds. This warrant further isolation of the compounds for chemotherapeutic purposes.Keywords: Antidesma bunius, Bugnay, Cancer, Cytotoxicity, Radical

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Section: Discussionmentioning

confidence: 99%

Free Radical Scavenging and In vitro Cytotoxic Activity of Bugnay (Antidesma bunius) Leaves Extract against A549 Human Lung Adenocarcinoma and HCT-116 Human Colorectal Cancer Cell Lines

Geronimo

Bancual

et al. 2020

Indones J Cancer Chemoprevent

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“…DPPH has been used for measurement of the free-radical scavenging ability of antioxidants. Reduction of an alcoholic DPPH solutio − in the presence of a hydrogen-donating antioxidant is the mainly step of this method. Hydrogen atom or electron-donation ability of the tested compounds were measured spectrophotometrically from the bleaching of the purple-colored ethanol solution of 2,2-diphenyl-1-picrylhydrazyl (DPPH).…”

Section: Methodsmentioning

confidence: 99%

Nitrophenyl-Group-Containing Heterocycles. I. Synthesis, Characterization, Crystal Structure, Anticancer Activity, and Antioxidant Properties of Some New 5,6,7,8-Tetrahydroisoquinolines Bearing 3(4)-Nitrophenyl Group

et al. 2022

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Regioselective cyclocondensation of 2,4-diacetyl-5hydroxy-5-methyl-3-(3-nitrophenyl/4-nitrophenyl)cyclohexanones 1a,b with cyanothioacetamide afforded the corresponding 7-acetyl-4-cyano-1,6-dimethyl-6-hydroxy-8-(3-and -4-nitrophenyl)-5,6,7,8tetrahydrosoquinoline-3(2H)-thiones 2a,b. Reaction of compounds 2a,b with ethyl iodide, 2-chloroacetamide ( 4a), or its Naryl derivatives 4b−e in the presence of sodium acetate trihydrate gave 3-ethylthio-5,6,7,8-tetrahydroisoquinoline 3 and (5,6,7,8tetrahydroisoquinolin-3-ylthio)acetamides 5a−i, respectively. Cyclization of compounds 5b−d,f,g into their isomeric 1-amino-6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamides 6b−d,f,g was achieved by heating in ethanol containing a catalytic amount of sodium carbonate. Structures of all synthesized compounds were characterized on the basis of their elemental analyses and spectroscopic data. The crystal structure of 5,6,7,8-tetrahydroisoquinoline 5d was determined by X-ray diffraction analysis. In addition, the biological evaluation of some synthesized compounds as anticancer agents was performed, and only six compounds showed moderate to strong activity against PACA2 (pancreatic cancer cell line) and A549 (lung carcinoma cell line). Moreover, the antioxidant properties of most synthesized compounds were examined. The results revealed high antioxidant activity for the most tested compounds.

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“…DPPH has been used for measurement of free radical scavenging ability of antioxidants. Reduction of an alcoholic DPPH solution [29][30][31] in the presence of a hydrogen-donating antioxidant is the mainly step of this method. Hydrogen atom or electron-donation ability of the tested compounds were measured spectrophotometrically from the bleaching of the purple-colored ethanol solution of 2,2-diphenyl-1picrylhydrazyl (DPPH).…”

Section: Cytotoxic Activtymentioning

confidence: 99%

Nitrophenyl-group-containing Heterocycles. Part I. Synthesis, Characterization, Anticancer Activity and Antioxidant Properties of Some New 5,6,7,8-tetrahydro-isoquinolines Bearing 3(4)-nitrophenyl Group

Sayed¹,

Hassanien

Farhan

et al. 2022

Preprint

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Regioselective cyclocondensation of 2,4-diacetyl-5-hydroxy-5-methyl-3-(3-nitrophenyl/4-nitrophenyl)cyclohexanones 1a,b with cyanothioacetamide afforded the corresponiing 7-acetyl-4-cyano-1,6-dimethyl-6-hydroxy-8-(3-nitrophenyl/4-nitrophenyl)-5,6,7,8-tetrahydro-soquinoline-3(2H)-thiones 2a,b in 93-96% yield. Reaction of compounds 2a,b with some ethyl iodide, 2-chloroacetamide (4a) or its N-aryl derivatives 4b-e by refluxing in ethanol, in the presence of slightly excess molar amounts of sodium acetate trihydrate, for one hour gave 3-ethylthio-5,6,7,8-tetrahydro-isoquinoline 3 and (5,6,7,8-tetrahydroisoquinolin-3-ylthio)acetamides 5a-i, respectively. On heating compounds 5b-d,f,g in ethanol containing a catalytic amount of sodium carbonate, they converted into their isomeric 1-amino- 6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamides 6b-d,f,g. Structural formulae of all synthesized compounds were characterized on the basis of their elemental analyses and spectroscopic data. Also, the biological evaluation of the synthesized isoquinolines as anticancer and antioxidant agents have been carried out and the obtained results are reported herein.

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Study of antioxidant activity of pyrimidinium betaines by DPPH radical scavenging method

Cited by 20 publications

References 17 publications

Free Radical Scavenging and In vitro Cytotoxic Activity of Bugnay (Antidesma bunius) Leaves Extract against A549 Human Lung Adenocarcinoma and HCT-116 Human Colorectal Cancer Cell Lines

Free Radical Scavenging and In vitro Cytotoxic Activity of Bugnay (Antidesma bunius) Leaves Extract against A549 Human Lung Adenocarcinoma and HCT-116 Human Colorectal Cancer Cell Lines

Nitrophenyl-Group-Containing Heterocycles. I. Synthesis, Characterization, Crystal Structure, Anticancer Activity, and Antioxidant Properties of Some New 5,6,7,8-Tetrahydroisoquinolines Bearing 3(4)-Nitrophenyl Group

Nitrophenyl-group-containing Heterocycles. Part I. Synthesis, Characterization, Anticancer Activity and Antioxidant Properties of Some New 5,6,7,8-tetrahydro-isoquinolines Bearing 3(4)-nitrophenyl Group

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