Free Radical Scavenging and In vitro Cytotoxic Activity of Bugnay (Antidesma bunius) Leaves Extract against A549 Human Lung Adenocarcinoma and HCT-116 Human Colorectal Cancer Cell Lines

Geronimo, Arnold Joseph O.; Bancual, Mari Erika Joi F.; Ko, Karl Anthony L.; Soliba, Lycon Marie L.; Ildefonso, Jeric John C.; Soriano, Angelica Mae B.; Tagalog, Alta Christine Marie M.; Acosta, Nikolai E.; Ang, Vincent S.; Apigo, Mariebon A.; Buenconsejo, Dana Grace S.; Chan, Joanna Kaitlin G.; Gresh, Cassandra B.; Santos, Crisel B.; Buñag, Leo Emmanuel; Domantay, John Anthony

doi:10.14499/indonesianjcanchemoprev11iss3pp124-133

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…However, this strain was repeatedly reported from marine sources, such as sediment samples (Wu et al, 2021), sponges, and deep-sea coral (Hu et al, 2022;Shin et al, 2016;Yao et al, 2021). Nemania bipapillata was recently reported as an endophyte of Vaccinium dunalianum Wight leaves along with P. capitalensis (Fan et al, 2020). It was also found in a mutualistic relationship with Diospyros crassiflora (Douanla-Meli and Langer, 2012).…”

Section: Discussionmentioning

confidence: 97%

“…It was also found in a mutualistic relationship with Diospyros crassiflora (Douanla-Meli and Langer, 2012). Meanwhile, an endophytic X. feejeensis was isolated previously from various host plants, such as an Euphorbiaceae plant, Sapium macrocarpum (García-Méndez et al, 2016), and from medicinal plants Hintonia latiflora (Rivera-Chávez et al, 2015) and Eryngium foetidum (Siriwach et al, 2011). The genus Phyllosticta is known to inhabit a wide host range (Zhu et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Antibacterial and cytotoxic secondary metabolites from endophytic fungi associated with Antidesma bunius leaves

Ariantari¹,

Putra²,

Leliqia³

et al. 2023

J Appl Pharm Sci

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Endophytic fungi isolated from Antidesma bunius leaves were investigated in this study. Six fungal endophytes were identified as Penicillium steckii AAB-01, Nemania bipapillata AAB-02, Xylaria feejeensis AAB-03, Hypomontagnella monticulosa AAB-04, Daldinia eschscholtzii AAB-05, and Phyllosticta capitalensis AAB-06. All of the isolated endophytic fungi were subjected to fermentation on rice media, followed by extraction with ethyl acetate. When tested for antibacterial activity, P. steckii AAB-01 extract showed the most potent inhibition against Staphylococcus aureus ATCC 6538 and Staphylococcus epidermidis ATCC 12228. Toxicity screening employing brine shrimp lethality test (BSLT) revealed potential toxicity of P. steckii AAB-01 and X. feejeensis AAB-03 extracts. Further investigation showed P. steckii AAB-01 extract had the highest inhibition toward MCF-7 cells, while D. eschscholtzii AAB-05 extract revealed the strongest cytotoxicity against 4T1 cells. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis tentatively identified five compounds, where indole acetic acid, O-acetylharmol, and oxindole I were suggested as the major metabolites in P. steckii AAB-01 extract, while 6-hydroxymelatonin and 2-methyl-6pentadecylpyridine were suggested as the major metabolites in D. eschscholtzii AAB-05 extract. This is the first report on LC-MS/MS identification of the main metabolites from bioactive extracts of P. steckii AAB-01 and D. eschscholtzii AAB-05 isolated from the leaves of A. bunius, which might contribute to the antibacterial and cytotoxic properties of the extracts. Thus, a detailed investigation of antibacterial and cytotoxic metabolites from these endophytes merits further studies.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Antibacterial and cytotoxic secondary metabolites from endophytic fungi associated with Antidesma bunius leaves

Ariantari¹,

Putra²,

Leliqia³

et al. 2023

J Appl Pharm Sci

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“…In our study, saponins were detected in aqueous/methanol extract. Geronimo et al ( 42 ) showed that the saponins inhibited the cellular growth of different cancer cell lines. Saponins and tannins possessed antitumor properties in MCF-7 and HCT-116 ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory and Anticancer Activities of Barley Bran Grown in Jordan: An in vitro and in vivo Study

Abuarab

Talib

2022

Front. Nutr.

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The Mediterranean diet is regarded as one of the most healthful dietary patterns in the world, owing to a combination of foods high in antioxidants and anticancer constituents. Barley bran is one of the components of the Mediterranean diet. It has nutritional and beneficial effects in different pathological conditions. Many studies were achieved to assess the nutritious values of barley bran, but there is no research indicating immunomodulatory and anticancer activities of barley bran grown in Jordan. The present study aims to examine and assess the potential immunomodulatory and anti-tumor activities of ethanol, n-hexane, aqueous/methanol, and water extracts obtained from barley bran. The Maceration method was utilized to prepare ethanol, n-hexane, aqueous/methanol, and water extracts. Various phytochemical groups were determined by using qualitative phytochemical tests. The antiproliferative activity of extracts was determined against MCF-7, HCT-116, A549, and EMT6/p by the MTT assay. The Folin-Ciocalteu reagent was used to detect the total phenolic content in extracts. Furthermore, immunomodulatory activity was assessed by determining the effect of extracts on splenocytes proliferation in the presence and absence of mitogens. The nitro blue tetrazolium assay and the neutral red method were used to assess the effect of each extract on the phagocytic activity of macrophages and pinocytosis, respectively. For the in vivo part, three different concentrations (10, 20, and 30% w/v) of barley bran were used to test the prophylactic effect in four Balb/C mice groups inoculated with EMT6/p cell-line subcutaneously. Also, serum samples were collected to assess the effect on cytokines (IFN-gamma, IL-2, IL-4, and IL-10). Barley bran extracts inhibited cancer cell proliferation. According to immunoassays, n-hexane and aqueous/methanol extracts could significantly rise lymphocyte proliferation and pinocytosis activity of macrophages. The activity of phagocytosis was increased by n-hexane and ethanol extracts. For the in vivo part, the average tumor size and weight of mice given the 30% barley bran group was significantly reduced (p < 0.05) compared with the control group. During our study, higher levels of TH1 cytokines (IFN- γ, IL-2) and lower levels of TH2 cytokine (IL-4) and T regulatory cytokine (IL-10) were obtained due to consumption of barley bran in food. Barley bran can be used as a prophylactic agent because it has anti-cancer and immunomodulatory activities.

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“…The bioactivity of A. bunius as anti-cancer is often associated with its activity as an antioxidant. The A. bunius leaf extract showed cytotoxic activity against human colorectal HCT-116 and human lung adenocarcinoma A549 (Geronimo et al, 2020). The A. bunius fruit secondary metabolites affect mitochondrial integrity and can modulate stress-responsive genes in cancer cells.…”

Section: Anti-cancermentioning

confidence: 99%

Antidesma bunius (L.) Spreng. (Foodstuffs and Its Bioactivity)

Silalahi

Purba²,

Sawitri³

et al. 2022

J Trop Etnobiol

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Antidesma bunius has been long used as food and traditional medicine by vary local communities in Indonesian. This study aims to explain the potential of A. bunius as a food ingredient and its bioactivity. The method used is survey and library research published online at Google Scholar by using keywords such as A. bunius, bioactivities of A. bunius and uses of A. bunius. Antidema bunius is an indigenous plant of Indonesia and has been cultivated in the yard as a shade and source of fruit. The local people in West Java have been used the A. bunius fruits for rujak ingredient which called as rujak buni. As a food ingredient, A. bunius fruit is used as an ingredient for salads and has been processed to be syrup, jelly, sauce and wine. The bioactivity of A. bunius is as an antioxidant, anti-bacterial, anti-cancer, anti-diabetic mellitus, anti-inflammatory and anti-cholesterol. The fruits of A. bunius has potential to be developed as a nutraceutical, especially as an anti-cancer and antioxidant-rich food

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Free Radical Scavenging and In vitro Cytotoxic Activity of Bugnay (Antidesma bunius) Leaves Extract against A549 Human Lung Adenocarcinoma and HCT-116 Human Colorectal Cancer Cell Lines

Cited by 4 publications

References 31 publications

Antibacterial and cytotoxic secondary metabolites from endophytic fungi associated with Antidesma bunius leaves

Antibacterial and cytotoxic secondary metabolites from endophytic fungi associated with Antidesma bunius leaves

Immunomodulatory and Anticancer Activities of Barley Bran Grown in Jordan: An in vitro and in vivo Study

Antidesma bunius (L.) Spreng. (Foodstuffs and Its Bioactivity)

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