Study of amylases recovery from maize malt by ion-exchange expanded bed chromatography

Biazus, Joana Paula Menezes; Severo, João Baptista; Santana, José Carlos Curvelo; Souza, Roberto Rodrigues de; Tambourgi, Elias Basile

doi:10.1016/j.procbio.2006.03.025

Cited by 24 publications

(39 citation statements)

References 16 publications

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“…On the other hand, RTDs were studied to determine liquid mixing and dispersion within the column (Amersham Biosciences, 1998, Jahanshahi et al, 2002Palsson et al, 2001;Levenspiel, 1972). These parameters were obtained by using equations 1 and 2 (Levenspiel, 1972;Najafpour, 2007;Jahanshai et al, 2003;Biazus et al, 2006).…”

Section: Measurements Of Rtdmentioning

confidence: 99%

Investigation of hydrodynamic parameters in a novel expanded bed configuration: local axial dispersion characterization and an empirical correlation study

Taheri

Jahanshahi

Mosavian

et al. 2012

Braz. J. Chem. Eng.

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-Study of liquid behavior in an expanded bed adsorption (EBA) system is important for understanding, modeling and predicting nanobioproduct/biomolecule adsorption performance in such processes. In this work, in order to analyze the local axial dispersion parameters, simple custom NBG (Nano Biotechnology Group) expanded bed columns with 10 and 26 mm inner diameter were modified by insertion of sampling holes. Based on this configuration, the particles and liquid can be withdrawn directly from various axial positions of the columns. Streamline DEAE particles were used as solid phase in this work. The effects of factors such as liquid velocity, viscosity, settled bed height and column diameter on the hydrodynamic parameters were investigated. Local bed voidages in different axial bed positions were measured by a direct procedure within the column with 26 mm diameter. Increasing trend of voidage with velocity at a certain position of the bed and with bed height at a certain degree of expansion was observed. Residence time distribution (RTD) analysis at various bed points showed approximately uniform hydrodynamic behavior in the column with 10 mm diameter while a decreasing trend of mixing/dispersion along the bed height at a certain degree of expansion was seen in the column with 26 mm diameter. Also lower mixing/dispersion occured in the smaller diameter column. Finally, a combination of two empirical correlations proposed by Richardson-Zaki and Tong-Sun was successfully employed for identification of the bed voidage at various bed heights (RSSE=99.9%). Among the empirical correlations presented in the literatures for variation of the axial dispersion coefficient, the Yun correlation gave good agreement with our experimental data (RSSE=87%) in this column.

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Section: Measurements Of Rtdmentioning

confidence: 99%

Investigation of hydrodynamic parameters in a novel expanded bed configuration: local axial dispersion characterization and an empirical correlation study

Taheri

Jahanshahi

Mosavian

et al. 2012

Braz. J. Chem. Eng.

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“…Na sequência, a eluição das enzimas é feita com o NaCl 0,25M, fazendo com que estas se desprendam das resinas (o que faz aparecerem os pequenos picos), sendo assim purificadas. e guardado a 5 °C (BIAZUS et al, 2005a(BIAZUS et al, , 2005b(BIAZUS et al, , 2006a(BIAZUS et al, , 2006b(BIAZUS et al, , 2006c(BIAZUS et al, , 2007CURVELO-SANTANA, 2003;FERREIRA et al, 2007;SEVERO Jr. et al, 2007;TOLEDO et al, 2007).…”

Section: Adsorção Em Leito Expandidounclassified

“…As α-amilase (EC 3.3.1.1; α-1,4 glicano, 4 -glicanohidroxilase) e β-amilase (EC 3.2.1.1, α-1,4, glicanomaltohidrolase) são enzimas que hidrolisam ligações α-1,4 não terminais de moléculas de amilose, amilopectina, glicogênio e dextrinas, gerando glicose, maltose, trioses e outras oses, porém não atuando sobre as ligações α-1,6. Seus pHs ótimos estão entre 4,5 e 6,5 e suas temperaturas ótimas estão compreendidas entre 55 e 75 °C, enquanto que suas massas molares variam para a α-amilase de 50 a 120 kDa e de 22 a 50 kDa para a β-amilase, dependendo das suas origens (AGUILAR et al, 2000;BIAZUS et al, 2006aBIAZUS et al, , 2007BLASQUEZ et al, 1998;FURUYA et al, 1995FURUYA et al, , 1996REGULY, 1996;WISEMAN, 1987).…”

Section: Introductionunclassified

“…O milho (Zea mays) é um produto agrícola muito difundido no Brasil, comercializado in natura, ou pouco processado e é vendido a baixo preço. A obtenção das enzimas α e β-amilases do seu malte gerariam um produto de alto valor agregado (BIAZUS et al, 2006a(BIAZUS et al, , 2007FERREIRA et al, 2007;CURVELO-SANTANA, 2003;SEVERO Jr. et al, 2007).…”

Section: Introductionunclassified

“…Rendimento da purificação: a eficiência dos processos foi avaliada por dois parâmetros, dados pelas Equações 1 e 2. A primeira é a recuperação da atividade enzimática (RA) que compara a atividade total no material purificado com a atividade total no material bruto (dado em porcentagem) e a segunda é o fator de purificação (FP) que compara a atividade enzimática específica total no material purificado com a atividade enzimática específica total no material bruto (BIAZUS et al, 2006a(BIAZUS et al, , 2007SANTOS, 2001;TOLEDO et al, 2007), como Equações 1 e 2:…”

Section: Introductionunclassified

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Purificação de amilases de malte de Zea mays

Biazus¹,

Santana²,

Souza³

et al. 2010

Ciênc. Tecnol. Aliment.

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Hydrodynamic Characteristics and Adsorption Particularity of Nanobiological Feedstock Along the Bed Height in a Novel Chromatography Column

2012

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Expanded bed adsorption (EBA) is a practical method for the separation of nanoparticulates. In order to analysis the local hydrodynamic and adsorption behavior of nanoparticle (NP)-based biological feedstock, a modified Nano Biotechnology Group EBA column with a 26-mm inner diameter was used to withdraw liquid from different axial positions of the column. Fabricated egg albumin (EA) NPs with an average size of 70 nm were employed as a model system and viral size/charge mimic to assess the relationship between hydrodynamic and adsorption performance of NPs at the different column regions. The effects of influential factors, including flow velocity and initial concentration of NPs, on NP hydrodynamic behavior and adsorption kinetics along the bed height were investigated. NP hydrodynamic studies confirmed that non-uniform behavior dominated the system and a decreasing trend of liquid mixing/dispersion with increase of bed height was observed in this column. The results demonstrated an increase in the mixing/dispersion at certain bed heights with the increase in both the velocity and feed initial concentration. Breakthrough curves were measured at various column points to determine the adsorption performance [dynamic binding capacity (DBC) and yield] in different bed positions/zones. Yield and DBC of NPs were improved along the bed height, whereas liquid velocity had the opposite effect. Increasing the initial concentration of NPs enhanced only the DBC. Separation of EA NPs under optimal conditions was 87 %, which is an excellent result for a one-pass frontal chromatography method. KeywordsEBA chromatography Á Modified NBG column Á EA NPs Á Axial hydrodynamic behavior Á Axial adsorption performance List of symbols B o Bodenstein number C Exit concentration of NPs in bed effluent (kg m -3 ) C o Initial concentration of NPs (kg m -3 ) C 9Exit concentration that has left the 9-cm sample port and is measured at this port (kg m -3 ) C 12Exit concentration that has left the 12-cm sample port and is measured at this port (kg m -3 )Volume of feed loaded onto the column until C 9 reaches a certain degree of NP breakthrough (m 3 ) V f,12Volume of feed loaded onto the column until C 12 reaches a certain degree of NP breakthrough (m 3 ) V l Liquid volume above the particles in the sampling syringe (m 3 ) V L,0-9 Liquid volume present in the bed voidage for the 0-to 9-cm column zone (m 3 ) V L,0-12 Liquid volume present in the bed voidage for the 0-to 12-cm column zone (m 3 ) V s,0-9 Settled volume of adsorbent in the 0-to 9-cm column zone (m 3 ) V s,9-12 Settled volume of adsorbent in the 9-to 12-cm column zone (m 3 ) V o Volume of the particles in the sampling syringe (m 3 )Greek letters e Bed voidage e i Local bed voidage at ith layer of bed d Variance (s)

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Study of amylases recovery from maize malt by ion-exchange expanded bed chromatography

Cited by 24 publications

References 16 publications

Investigation of hydrodynamic parameters in a novel expanded bed configuration: local axial dispersion characterization and an empirical correlation study

Investigation of hydrodynamic parameters in a novel expanded bed configuration: local axial dispersion characterization and an empirical correlation study

Purificação de amilases de malte de Zea mays

Hydrodynamic Characteristics and Adsorption Particularity of Nanobiological Feedstock Along the Bed Height in a Novel Chromatography Column

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