Study of alternate cooling and heating treatment induced tumor microvasculature injury

Abstract

Tumor microvasculature is important to tumor growth, metastasis and thus tumor treatment outcome. The alternate cooling and heating treatment has been confirmed to have advantages over single treatment of cooling or heating. The degree of tumor microvasculature damage induced by the alternate cooling and heating treatment and the mechanisms underlying are studied in this paper. The response of the tumor microvasculature to different treatments including alternate cooling and heating is observed in vivo through confocal microscopy using the nude mice dorsal skin fold tumor chamber model. Results show that alternate cooling and heating has induced much more severe damage to the microvessel structure throughout the entire tumor. Numerical simulations of the mechanical stresses on the tumor vessel wall has found that during the alternate treatment, the vessel wall suffer a rapid chang in thermal stresses in the opposite directions successively, which might caused damage to the peripheral microvasculature and micro-cracks in the central vessels. Reperfusion of blood flow after freezing also led to relatively large stresses on the vessel wall, especially when blood flow re-perfuses quickly during the subsequent heating. The quick increase of stresses on the blood vessel might be one of the key issues causing the blood vessel rupture referring to the experimental observation. The preliminary study has partly revealed the mechanism underlying serious tumor microvasculature damage caused by the alternate cooling and heating treatment.alternate cooling and heating treatment, tumor microvasculature injury, thermal stress, blood flow Citation:Shen Y Y, Zhang A L. Study of alternate cooling and heating treatment induced tumor microvasculature injury.

Synergetic Thermal Therapy for Cancer: State-of-the-Art and the Future

“…Combined multiple thermal therapies are showing up in recent years, bringing brand new discoveries. Greatly enhanced immune effects have been discovered through combined cryoablation and thermal therapy [ 69 , 146 , 147 , 148 , 149 ]. Such combined therapy could create an inflammatory environment for stimulation of T cell activation and differentiation, and thus promoting long-term antitumor immunity through inducing the release of tumor-related DAMPs, especially tumor-related HSP70 in situ and the peripheral [ 150 ], down-regulating TNF- levels [ 151 ], and releasing iron [ 152 ].…”