Study of adsorption and viscoelastic properties of proteins with a quartz crystal microbalance by measuring the oscillation amplitude

Anti-rabbit immunoglobulin G detection in complex medium by PM-RAIRS and QCM

“…BSA is an efficient blocking agent and does not bind to protein A (Galli Marxer et al, 2003). After covalent grafting of PrA, treatment of the surface with the solution of BSA led to an increase (+30%) of the PM-RAIRS amide I + II band area (see Fig.…”

“…direct physisorption (Bizet et al, 1998;Caruso et al, 1996;Chang et al, 1995), covalent binding (Babacan et al, 2000;Caruso et al, 1996;Cui et al, 2003b;Disley et al, 1998;Eun et al, 2002;Frederix et al, 2004;Grubor et al, 2004;Lin and Tsai, 2003;Pei et al, 2001), or affinity binding via the biotin/avidin couple (Boozer et al, 2003;Cui et al, 2003a;Ouerghi et al, 2002) or via Staphylococcus aureus Protein A (PrA), two of them were chosen in the present work and compared. Several examples of immunosensing devices based on the immobilisation of specific antibodies via a layer of PrA have been reported (Babacan et al, 2000;Galli Marxer et al, 2003;Grubor et al, 2004;Kaur et al, 2004;Lin and Tsai, 2003;Lu et al, 2000;Michalzik et al, 2005;Muramatsu et al, 1987;Saha et al, 2003); the benefit is a proper orientation of the antibody (Lu et al, 1996) and an easy immunosensor regeneration (Quinn et al, 1999).…”

“…Binding of proteins to surfaces induces frequency shifts due to the molecules themselves but also to hydrodynamically coupled water and/or modifications in the viscoelastic properties of the adlayers (Fredriksson et al, 1998;Galli Marxer et al, 2003;Gry Hemmersam et al, 2005;Höök et al, 2001Höök et al, , 2002Lord et al, 2006;Olsen et al, 2003;Voivona et al, 2002). If the added overlayer slips at the interface with the quartz crystal, or if it is "soft" enough to be reversibly strained by the shear motion, it indeed induces viscosity changes detectable via the measurement of dissipation (Höök et al, 1998;Rodahl et al, 1996).…”

Development of QCM biosensor to detect a marine derived pathogenic bacteria Edwardsiella tarda using a novel immobilisation method

“…The strategy chosen to create the sensing layer should enable to control both the amount and the orientation of the antibody on the transducer while preserving its bioactivity (Briand et al, 2007). Thus many published papers showed an immobilisation technique based on Staphylococcus aureus protein A coating (Babacan et al, 2000;Galli Marxer et al, 2003;Grubor et al, 2004;Kaur et al, 2004;Michalzika et al, 2005).…”

Blood Coagulation Testing Smartphone Platform Using Quartz Crystal Microbalance Dissipation Method

“…There were two major parameters could be traced when using QCM sensors: frequency and dissipation. Frequency shift was proportional to the mass change according to the Sauerbrey equation for a rigid, thin, uniform film and the dissipation change should be less than 1 × 10 −6 per 10 Hz frequency change [ 41 , 42 ]. While in the coagulation process the ratio of dissipation change to frequency change would go up to 2.5 × 10 −6 /10 Hz, as a result, the total adsorbed mass was easily underestimated [ 43 ] and the fluctuations might occur due to the non-uniform adhesion of fibrin onto the surface of the QCM electrode.…”