Study of acute trichinosis in Ghurkas: specificity and sensitivity of enzyme-linked immunosorbent assays for IgM and IgE antibodies to Trichinella larval antigens in diagnosis

Au, Arstila; Ko, Ronald C.; Simon, Julia; Ridell, N.J.; Wong, F.W.T.; Templer, M.J.

doi:10.1016/0035-9203(83)90175-x

Cited by 32 publications

(8 citation statements)

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“…I nfection with Trichinella spiralis evokes a humoral immune response, predominantly of the IgM, IgG and IgA classes (van Knapen et al, 1982;Au et al, 1983;Feldmeier et al, 1987). In this study we report An interesting result from our study is the detectable IgG4 response directed to a 45 kDa component of the ES antigen.…”

Section: Discussionsupporting

confidence: 57%

Antigen recognition by IgG4 antibodies in human trichinellosis

et al. 2001

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Summary:The antibody isotype response to Trichinella spiralis excretory/secretory (ES) products of muscle larva was examined using sera from patients with confirmed trichinellosis. Using Western blots we identify components of the ES antigen that are recognized by IgM and IgG antibodies. A 45 kDa component was strongly recognized by different antibody classes and subclasses. We observed a 45 kDa-specific lgG4 response that was detected exclusively using sera of patients with trichinellosis and not of patients with echinococcosis, filariasis, cysticercosis, ascariasis, strongyloidiasis or toxocariasis. These results are relevant for the diagnosis of human trichinellosis.KEYWORDS : human trichinellosis, ES antigen, 45 kDa, lgG4.M onitoring programs for detection of trichinellosis in slaughterhouse animals have dramatically decreased the incidence of human trichinellosis. However, in many parts of the world, including Europe, it continues to occur. Recent outbreaks of trichinellosis have been reported from Germany, France, Spain and Italy (Ancelle et al, 1998;Rodriguez et al., 1999); (Pozio 1998).In our laboratory the immunodiagnosis of trichinellosis is performed by using an enzyme-linked immunosorbent assay (ELISA) in which the excretory/secretory (ES) products of Trichinella spiralis muscle larvae are used as antigen (van Knapen et al., 1982 MATERIALS AND METHODST he excretory/secretory (ES) antigen was prepared using viable muscle larva of T. spiralis as previously described by Gamble (1985). The 45 kDa antigenic component was purified from freshly obtained muscle larva using two-step affinity chromatography as described by Homan et al. (1992) SERUM SAMPLES Serum samples from trichinellosis patients recently received at our laboratory and from an outbreak of trichinellosis that took place in Slupsk, Poland in 1991 were used in this study. The patients here studied were all at an early stage of trichinellosis. All serum samples used tested positive for IgM and/or IgG by means of ELISA using T. spiralis ES antigen. Infection was confirmed for several patients from the outbreak in Poland and for the other patients used in this study by identification of Trichinella larva in muscle biopsy.The source for T. spiralis infection for the outbreak was undercooked pork. Sera from our laboratory from echinococcosis, filariasis, cysticercosis, ascariasis, strongyloidiasis or toxocariasis patients were also included. WESTERN BLOTTING PROCEDUREThe ES antigen or the 45 kDa protein was solubilized under reducing conditions and electrephorized on Parasite, 2001, 8, S168-S171 ANTIGENS S168.

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Section: Discussionsupporting

confidence: 57%

Antigen recognition by IgG4 antibodies in human trichinellosis

et al. 2001

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“…However, fewer samples were analyzed than in the present study, and they were collected 1.5 and 4 months after infection. In studies in which the samples were collected shortly after infection, the sensitivity ranged from 75% to 94% (4,5,20,26).…”

Section: Discussionmentioning

confidence: 99%

“…Although a plethora of tests for the detection of IgG antibodies have been developed, the most commonly used test is an enzyme-linked immunosorbent assay (ELISA), given its sensitivity (13,14,19). This test was first developed using low-specificity crude worm extract antigens prepared from L1 larvae (4,11,12,44) and then using more-specific excretory/secretory antigens (ESA) prepared from L1 larvae maintained in culture (1,2,5,9,10,15,20,24,25,32,41,43,47).…”

Section: Trichinellosis (Formerly Known As Trichinosis or Trichiniasis)mentioning

confidence: 99%

Validation of an Enzyme-Linked Immunosorbent Assay for Diagnosis of Human Trichinellosis

Morales

Ludovisi

Amati

et al. 2008

Clin Vaccine Immunol

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Trichinellosis is a zoonotic disease caused by the consumption of raw or semiraw meat from different animals harboring Trichinella larvae in their muscles. Since there are no pathognomonic signs, diagnosis can be difficult; for this reason, serology is important. The objective of this study was to validate an enzyme-linked immunosorbent assay (ELISA) using excretory/secretory antigens to detect anti-Trichinella immunoglobulin G antibodies in human sera. A total of 3,505 human serum samples were tested. A receiver-operator characteristic (ROC) curve analysis was performed. The accuracy of the test was determined by calculating the area under the curve, which was equal to 0.999, indicating high accuracy. The coefficient of variation calculated for data from four serum samples in eight working sessions was no higher than 5% for the positive sera or 14% for the negative sera. Moreover, the analysis of the differences in optical density between duplicates indicated a high repeatability for the ELISA. At the ROC optimized cutoff, the sensitivity and specificity of the test were, respectively, 99.2% and 90.6% (specificity of 95.6% when excluding the samples from multiparasitized persons from Tanzania). The validated ELISA showed good performance in terms of sensitivity, repeatability, and reproducibility, whereas the specificity was limited. These results suggest that this test is suitable for detecting anti-Trichinella antibodies in human sera for diagnostic purposes, whereas its use in epidemiological surveys could be questionable.

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“…87 The data on specific IgE are contradictory. 78 85 86 Circulating antigens are also not completely reliable. 89 They were found in 47% of patients with clinical trichinellosis and in 13% of patients suspected of infection; healthy subjects with no history of trichinellosis were negative.…”

Section: Specific Findings (Immunodiagnosis)mentioning

confidence: 99%

New aspects of human trichinellosis: the impact of new Trichinella species

Bruschi

Murrell²

2002

Postgraduate Medical Journal

129

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Trichinellosis is a re-emerging zoonosis and more clinical awareness is needed. In particular, the description of new Trichinella species such as T papuae and T murrelli and the occurrence of human cases caused by T pseudospiralis, until very recently thought to occur only in animals, requires changes in our handling of clinical trichinellosis, because existing knowledge is based mostly on cases due to classical T spiralis infection. The aim of the present review is to integrate the experiences derived from different outbreaks around the world, caused by different Trichinella species, in order to provide a more comprehensive approach to diagnosis and treatment.

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Study of acute trichinosis in Ghurkas: specificity and sensitivity of enzyme-linked immunosorbent assays for IgM and IgE antibodies to Trichinella larval antigens in diagnosis

Cited by 32 publications

References 10 publications

Antigen recognition by IgG4 antibodies in human trichinellosis

Antigen recognition by IgG4 antibodies in human trichinellosis

Validation of an Enzyme-Linked Immunosorbent Assay for Diagnosis of Human Trichinellosis

New aspects of human trichinellosis: the impact of new Trichinella species

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