Study of a Nylon Bag Technique for In Vivo Estimation of Forage Digestibility

Keuren, R. W. Van; Heinemann, Wolfgang

doi:10.2527/jas1962.212340x

Cited by 64 publications

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“…An increase in sample weight tended to give lower degradabilities for barley and soybean meal also with the 40 pm bag (Bag B). This was especially true when a sample weight of 57-60 mg DM per cm 2 was used: the results agreed well with those of VAN KEUREN and HEINEMANN (1962) and UDEN et al (1974).…”

Section: Evaluation Of Feed Protein Degradation By Regression Analysissupporting

confidence: 81%

“…The samples were ground to the size normally offered to the animals. EHLE et al (1982) found disappearances of 27 and 17 % for DM and (1980) found particulate losses of 2-4 % after 1 hr incubation, in which timothyryegrass hay had particle sizes of 2,5 Both VAN KEUREN and HEINEMANN (1962) and UDEN et al (1974) reported that an increased sample weight decreased digestibility in the rumen, especially, if the ratio of sample weight to bag area was not constant.…”

Section: Evaluation Of Feed Protein Degradation By Regression Analysismentioning

confidence: 99%

“…Neither are the possible limits of the techniques described in the literature; although such factors as i.a. bag porosity (UDEN et al 1974), sample weight (VAN KEUREN andHEINEMANN 1962, UDEN et al 1974), and sample treatment (VAN KEUREN andHEINEMANN 1962, McMANUS et al 1972) have been reported as affecting the results obtained using the bag method.…”

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confidence: 99%

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The nylon bag technique in the determination of ruminal feed protein degradation

Setälä

1983

AFSci

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The investigation included experiments in which factors affecting the reliability of the nylon bag method were studied. The possibility of applying the feed protein degradabilities to practical feeding conditions was also examined. In the experiments concerning reliability, such factors as bag porosity, sample weight, sample treatment, washing procedure, diets, and differences between animals and incubation days were studied. The feed protein degradabilities were also determined by using as incubation periods the ruminal retention times for particulate matter of different feeds, evaluated as a function of DM intake/100 kg liveweight in different diets. A nylon bag, with a pore size of 40 µm and internal dimensions of 6 X 12 cm was selected for the degradability determinations. The sample weight used in incubations was 57 —60 mg DM/cm2. In the determination of feed protein degradability, when sheep are used as experimental animals, it is recommended that for routine determinations only one animal be used, analyzing the contents of two bags for each incubation period during two successive days. A control sample of which degradability is determined in advance in many sheep, should be used in all incubations in order to control the digestive processes in the rumen of the experimental sheep. The actual degradabilities analyzed by the bag method are applicable in practise, if they are determined using animals at similar feeding levels and on diets similar to those prevailing under the conditions in which the degradabilities are going to be used.

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Section: Evaluation Of Feed Protein Degradation By Regression Analysissupporting

confidence: 81%

Section: Evaluation Of Feed Protein Degradation By Regression Analysismentioning

confidence: 99%

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The nylon bag technique in the determination of ruminal feed protein degradation

Setälä

1983

AFSci

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“…The granular precipitated lignin was washed with hot water and dried to constant weight. Dry-matter digestibility was estimated by the nylon bag, rumen digestion technique of Keuren & Heineman (1962). While this technique does not necessarily give values equal to conventional in vivo digestibility values, it is considered a valid technique to determine the relative digestibilities of feeds.…”

Section: Introductionmentioning

confidence: 99%

A study of various chemical treatments to remove lignin from coarse roughages and increase their digestibility

Chandra

Jackson

1971

J. Agric. Sci.

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Several delignifying agents, used in various paper-making processes, have been compared for their ability to remove lignin and increase the digestibility of several roughages in laboratory experiments using a spray method of treatment recently suggested by Wilson & Pigden (1964). The chemicals were dissolved in small quantities of water which were then used to moisten the roughage. No washing was done after treatment. Sodium hydroxide was the most effective chemical; at a rate of 10 g per 100 g of roughage, the lignin content of ground maize cobs was reduced by 26 % and rumen dry-matter digestibility increased by more than 100 %. Bleaching powder was as effective as sodium hydroxide in removing lignin, but residual chlorine apparently inhibited rumen microbial digestion.Maize cobs, wheat and paddy straws, sorghum stover and sugar cane tops, though of widely differing initial digestibility, were brought to an equal level of rumen digestibility if treated with 4 % or more of sodium hydroxide. Drying the treated roughage at 100 degrees instead of at room temperature increased digestibility only in the case of maize cobs. The alkali spray treatment was as effective in increasing the digestibility of chaffed as of ground wheat straw. Data are presented which show the amounts of residual alkali in treated roughage as a function of time after treatment and of concentration of alkali used in the treatment. Further, data are presented which show the loss of nutrients from treated roughage if washed as in the traditional method.

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“…Several studies have been made to ascertain whether loss of feed frorn nylon bags in the rumen of cattle could be used to estimate digestibility (4,5,7,9). However, since the nylon bag technique does not reproduce exactly the processes involved in normal digestion, relationships found between apparent digestibility and loss from bags are not reproducible under all conditions.…”

Section: Iniroductionmentioning

confidence: 99%

Maximum Loss of Feed From Nylon Bags in the Rumens of Steers as Related to Apparent Digestibility

Bailey¹,

Hironaka²

1970

Can. J. Anim. Sci.

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Nylon bags containing rolled barley or mix-and all-barley rations, respectively. The cortures of rolled barley and finely chopped alf-responding values for maximum protein loss alta hay in ratios of 1:1, 2:,1, and 3:1 were were 95.3,94.2,94.6,and98.8% while those removed from two fistulated steers 1, 2, 4, 8, for apparent protein digestibility were 76.8, 14,24, 48, and,72 Another approach would be to find some characteristic that is more reproducible than moasurements of the amount of feed lost after a predetermined time interval. In a previous exporiment the rate of loss of dried grass from nylon bags declined steadily after the first hour, and the total loss appeared to approach a maximum value ( 1). If such a maximum could be measured, it might be a useful index of digestibility. We reasoned that the asymptote of the line of best fit through the cumulative loss curve would be an estimate of tho theoretical maximum loss of any feed from nylon bags. In this paper we present results that describe the cumulative loss of fo'ur different diets from nylon bags and cornpare the calculated maximum loss with apparent digestibilities of the same feeds measured in the usual way. MATERIAIS AND METHODS RationsFour rations were used; three were mixed steam-rolled badey and alfalfa hay in ratios of "1,:"1,,2:1, and 3:1, and the fourth was barley alone. Enough linseed oil meal was added to the barley in all rations to raise the protein content to that of the hay, so that each ration would have the same crude protein content (l4.5Vo). The protein content of the barley was l3.2Vo. Gan. J. Anim. Sci.50:325-330 (Aug. 1970) 325 Can. J. Anim. Sci. Downloaded from www.nrcresearchpress.com by 18.236.120.13 on 05/11/18For personal use only.

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Study of a Nylon Bag Technique for In Vivo Estimation of Forage Digestibility

Cited by 64 publications

References 0 publications

The nylon bag technique in the determination of ruminal feed protein degradation

The nylon bag technique in the determination of ruminal feed protein degradation

A study of various chemical treatments to remove lignin from coarse roughages and increase their digestibility

Maximum Loss of Feed From Nylon Bags in the Rumens of Steers as Related to Apparent Digestibility

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