The nylon bag technique in the determination of ruminal feed protein degradation

Abstract. The effect of wood molasses ensiled barley on ration digestibility and nitrogen utilization (Exp. 1 and Exp. 2) and on rumen fermentation and degradation rate in sacco (Exp. 2) was investigated in two experiments. The ration contained 0.9 kg of DM. The proportion of hay was 30 % in Exp. 1 and 40 % in Exp. 2. In Exp. I the experimental diets were dried barley (DB) and barley ensiled with a level of 8 (WMB8) or 16 % (WMBI6) wood molasses of barley DM. In Exp. 2 the diets were dried barley (DB), propionic acid-treated barley (PAB) and barleys ensiled with 0.3 % v/w <7o of AIV II solution (AIVB) or with wood molasses at a level of 12 % of barley DM (WMBI2).The ration digestibility decreased with increasing levels of wood molasses. The difference in DM and organic matter (OM) digestibility was significant (P < 0.05) between DB and WMBI6. Wood molasses tended to decrease the apparent digestibility of crude protein and crude fibre and to increase nitrogen retention. The percentages of nitrogen retained of ingested were in Exp. 1 on DB, WMBB and WMBI6 diets 13.1, 17.8 and 15.8 % and in Exp. 2 on DB, PAB, AIVB and WMBI2 diets 13.8, 13.2, 10.3 and 14.5 %, respectively.After feeding, the concentration of total VFA in the rumen was higher when ensiled barleys were fed. The proportion of propionic acid in the total VFA was greater with DB and PAB than with AIVB and WMBI2 diets (P < 0.01), and butyric acid was correspondingly lower (P < 0.01 and P > 0.05). The proportion of isovaleric and valeric acids was highest on AIVB diet. On DB and PAB diets the ammonia concentration in the rumen decreased after feeding, but on AIVB and WMBI2 diets the highest value was reached 1.5 hours after feeding. The degradation rate of DM and CP as determined by nylon bag method was faster on AIVB and WMBI2 than on DB and PAB diets.

Section: Rumen Fermentationsupporting

confidence: 89%

“…The DM and crude protein (CP) degradabilities of DB, PAB, AIVB and WMBI2 were determined in Exp. 2 by the method of Setälä (1983). The experimental design was 4x4 Latin square and the incubation times were 2,5, 9 and 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Wood molasses as a preservative for high moisture barley: 2. Ration digestibility and rumen fermentation in sheep

Huhtanen

1984

“…Samples of fresh silage were analysed for reducing sugars (Somogyi 1945, with modifications of Salo 1965, lactic acid (Barker and Summerson 1941), volatile fatty acids (Huida 1973), ammonia nitrogen (McCullough 1967), and for total and water soluble Kjeldahl nitrogen. Degradation of protein was determined in a sheep in sacco (Mehrez andORSKOV 1977, Setälä 1983). The incubation times of concentrates were 2,5, 8 and 24 hours, and an additional 48 hours for silage and hay.…”

Section: Sampling and Analytical Methodsmentioning

confidence: 99%

Rapeseed meal as a supplementary protein for dairy cows on grass silage-based diet, with the emphasis on the Nordic AAT-PBV feed protein evaluation system

Tuori

1992

The effect of rapeseed meal (RSM) supplementation on the performance of dairy cows on direct cut grass silage based diets was studied in five feeding trials. The proportion of RSM varied from 0% to 33% in the concentrate mixture (the grain was an oat-barley mixture of 1:1). In one experiment the treatments were RSM and soybean meal (SBM), while in another experiment forage was either grass silage or bam dried hay cut at the same maturity. In addition, this was compared to the data of other trials in Finland during the last ten years, in which RSM supplementation had been used. Using this data the response in terms of milk yield to RSM supplementation was estimated. The utilization of protein in milk production was estimated by the Nordic AAT-PBV protein evaluation system. During the experiments (1983-1990) the varieties of turnip rape were changed from high glucosinolate, containing single-zero, to low glucosinolate containing doublezero varieties, while the glucosinolate content was reduced from 40-50 μmoles to 14 μmoles per g of defatted meal. Heat-moisture treatment (™Öpex) further reduced the glucosinolate content by half. By replacing grain with RSM in the concentratemixture with ad libitum silage feeding, the silage intake increased by 0.43 kg per kg increase in RSM on the basis of dry matter (DM) (non significant). The response in increased milk production was 0.77 kg in milk or 0.70 kg in energy corrected milk (ECM) yield (P

“…Hay was analysed for in vitro digestibility according to Tilley and Terry (1963). In sacco rumen degradability of barley and wheat distillery by-products was determined in a nylon bag (porosity 40 /*m) as described by Setälä (1983).…”

Section: Methodsmentioning

confidence: 99%

Distillers dried by-products from barley as protein source for ruminants

Näsi

1984

Abstract. The nutritive value of barley-derived distillers dried grains (BDDG) and distillers dried grains with solubles (BDDGS) was assessed in digestibility and in sacco rumen degradability experiments. The crude protein contents of BDDG and BDDGS were 26.4 % and 29.9 %, and the crude fibre contents 19.9 % and 17.4 % in DM, respectively. The ADF and NDF contents averaged 33 % and 67 % and the lignin 7.6 % of DM. The digestibilities of the nutrients were measured with four rams in both trials and the distillers by-products were given at two levels, 33 % ad 66 %, in hay-based rations. The digestibilities in BDDG and BDDGS were, respectively, 43.1°/o and 47.7 % for organic matter, 64.5°/o and 66.5 % for crude protein, and 86.7 % and 87.9 % for ether extract. Due to the low digestibilities, the energy values of the two products were low, 0.58 FU/kg DM and 8.0 MJ/kg for both, and DCP/FU 295 g for BDDG and 347 g for BDDGS. The rumen degradability of the crude protein of BDDG and BDDGS was found to be low and significantly lower than the degradability of wheat distillery by-products (P < 0.05). Fine grinding gave more rapid degradability. Due to the low total digestibility and reduced lysine content, BDDG and BDDGS may have limited protein availability when given as supplements.