Study of a new bone-targeting titanium implant&amp;ndash;bone interface

Liu, Xiangning; Zhang, Ye; Li, Shaobing; Wang, Yayu; Sun, Ting; Li, Zejian; Cai, Lizhao; Wang, Xiaogang; Zhou, Lei; Lai, Renfa

doi:10.2147/ijn.s119520

Cited by 12 publications

(12 citation statements)

References 33 publications

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“…In animal studies the influence on mineralization, osteogenic markers and viability of quiet equal concentrations of SV were investigated upon cells particularly of rodent origin [21][22][23][24][25][26][32][33][34][35][36][37][38][39].…”

Section: Discussionmentioning

confidence: 99%

Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts

Sabandal¹,

Schäfer²,

Aed³

et al. 2020

Head Face Med

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Background: Frequently statins were administered to reduce the LDL-concentration in circulating blood. Especially simvastatin (SV) is an often prescribed statin. Pleiotropic effects of these drugs were reported. Thus, the aim of this study was to evaluate effects of SV on osteoblastic mineralization. Methods: After informed consent primary osteoblasts were collected from tissue surplus after treatment of 14 individuals in the Department of Cranio-Maxillofacial Surgery, University Hospital Münster. The cells were passaged according to established protocols. Viability, mineralization capability and osteoblastic marker (alkaline phosphatase) were determined at day 9, 13 and 16 after adding various SV concentrations (0.05 μM, 0.1 μM, 0.5 μM, 1.0 μM). Statistical analysis was performed using the Kruskal-Wallis-test. Results: The cell cultures showed a time and dose-dependent significantly decreased viability (p < 0.01) and a significantly increased mineralization (p < 0.01) in a late mineralization stage after adding SV. The typical alteration of the alkaline phosphatase (ALP) levels during osteogenic differentiation was not recognizable. Conclusions: The pleiotropic effects found for different SV concentrations were possibly originated from other mineralization pathways beside the ALP induced one. Additionally, possible alterations of protein expression levels during mineralization and investigation of possible deviating application of SV in other treatment fields can be considered after gaining a deeper insight in the affected mechanisms.

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Section: Discussionmentioning

confidence: 99%

Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts

Sabandal¹,

Schäfer²,

Aed³

et al. 2020

Head Face Med

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“…27 A dualcontrolled system, loading tetracycline grafted simvastatin-loaded polymeric micelles in TNTs, was constructed by Liu et al and reported to improve local bone regeneration and osseointegration. 28 On the other hand, titanium nano-nets (TNNs) topography is also extensively researched owing to the ECM-like structure and has demonstrated early and strong bone binding ability. [29][30][31] Alkali-heat treatment, involving immersion in sodium hydroxide solution and heating, is a common method to create TNNs topography with a sodium titanate layer.…”

Section: Introductionmentioning

confidence: 99%

Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration

Yu¹,

Xu²,

Zhang³

et al. 2021

IJN

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Background and Purpose: Micro-/nano-tubes (TNTs) and micro-/nano-nets (TNNs) are the common and sensible choice in the first step of combined modifications of titanium surface for further functionalization in the purpose of extended indications and therapeutic effect. It is important to recognize the respective biologic reactions of these two substrates for guiding a biologically based first-step selection. Materials and Methods: TNTs were produced by anodic oxidation and TNNs were formed by alkali-heat treatment. The original selective laser melting (SLM) titanium surface was set as control. Surface characterization was evaluated by scanning electron microscopy, surface roughness, and water contact angle measurements. Osteoclastogenesis and osteogenesis were measured. MC3T3-E1 cells and RAW 264.7 cells were used for in vitro assay in terms of adhesion, proliferation, and differentiation. In vivo assessments were taken on Beagle dogs with micro-CT and histological analysis. Results: TNN and TNT groups performed decreased roughness and increased hydrophilicity compared with SLM group. For biological detections, the highest ALP activity and osteogenesis-related genes expression were observed in TNT group followed by TNN group (P <0.05). Interestingly, when it comes to the osteoclastogenesis, TNNs displayed lowest TRAP activity and osteoclastogenesis-related genes expression and TNTs were lower than SLM but higher than TNNs (P <0.05). BV/TV around implants was highest in TNT group after 4 weeks (P <0.05). HE, ALP and TRAP staining showed that osteogenic and osteoclastic activity around TNTs were both higher than TNNs (P <0.05). Conclusion:TNNs and TNTs have dual advantages in promotion of osteogenesis and inhibition of osteoclastogenesis. Furthermore, TNNs showed better capability in inhibiting osteoclast activity while TNTs facilitated stronger osteogenesis. Our results implied that TNT substrates would take advantage in early application after implantation, while diseases with inappropriate osteoclast activity would prefer TNN substrates, which will guide a biologically based first-step selection on combined modification for different clinical purposes.

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“…TiO 2 nanotubes were first described in the nineties as having a "columnar honeycomb-like lattice, " observed upon addition of fluoride ionic species to the electrolyte in the anodization of Ti and Ti6Al4V (Zwilling and Darque-Ceretti, 1997). More recently, titania nanotubes have gained interest as bone contact surfaces mainly for two characteristic properties: a unique topography, to support early osteoblast adhesion and proliferation (Iwata et al, 2017) and the possibility to incorporate different classes of biologically-active molecules (either with osteogenic or antimicrobic activity) (Liu et al, 2016;Tao et al, 2019;Ion et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Gentamicin-Loaded TiO2 Nanotubes as Improved Antimicrobial Surfaces for Orthopedic Implants

et al. 2020

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Titanium oxide nanotube arrays are extremely promising materials for localized drug delivery in orthopedic implants due to their excellent properties and facile preparation. TiO 2 nanotubes can act as an effective drug reservoir to prevent bacterial colonization and implant infection and, at the same time, could promote tissue regeneration and effective osseointegration. Here, highly ordered TiO 2 nanotubes (NTs) were synthesized by electrochemical anodization of titanium foils and the process parameters were varied in order to obtain a large range of NT diameters to evaluate its influence. The effect of NTs' diameter on gentamicin loading/release and on osteosarcoma cell and bacterial adhesion was assessed. Anodization was confirmed as an easy and effective method to prepare highly ordered, open top nanotubes with predictable diameter as a function of imposed voltage. A lower amount of bacteria Staphylococcus Aureus adhesion was found on unloaded NTs surfaces at 24 h. When gentamicin was loaded, protracted release and antibacterial action was observed and bacteria adhesion was prevented on all NTs dimension. However, higher cell proliferation and a more favorable cell morphology was observed on smaller nanotubes, to support the indication toward a reduction in NTs diameter for the preparation of effective implant surfaces.

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Study of a new bone-targeting titanium implant–bone interface

Cited by 12 publications

References 33 publications

Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts

Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts

Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration

Gentamicin-Loaded TiO2 Nanotubes as Improved Antimicrobial Surfaces for Orthopedic Implants

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