Study Cytotoxicity and Genotoxicity of Hemolysin Using Human Lymphocyte

Abstract

Haemolysin is a toxic extracellular protein produced by many gram negatives (e.g., E. coli, Serratia spp). Proteus spp., Vibrio spp., Pasteurella spp., Pseudomonas aeruginosa) and bacteria gram-positive (e.g., streptococcus spp., Staphylococcus aureus, listeria spp., Bacillus cerius, Titanium Clostridium), all possess the potential of a particular nurse. Hemolycin has therefore always been considered virulent, most red blood cell decompositions cause red blood cells to decompose by forming pores with different diameters in the membrane and are assigned as such because they have the ability to decompose red blood cells (RBCs). Many hemolycene can also attack -possibly by a similar mechanism -other mammalian cells. Because of this cellular effect, it is also called cytolysins (Goebel et al., 1988).Studies in the living body and in the laboratory have provided some evidence that hemolycin leads to excessive secretion of inflammatory mediators by immune cells that promote inflammation caused by other bacterial virulent factors. Hemolycin has been shown to stimulate the secretion of interleukin-1φ and tumor necrosis factor α (TNF-α) from single human cells as early as a few decades ago, partially purified hemolycin has been shown to have metogenic ability However, some speculation has arisen that the toxins used in the studies may be contaminated by other factors that stimulate the glioblastoma of lymphocytes (Adam Bownik et al.,2008). Further studies on pure poison have shown that hemolycin actually stimulates the proliferation of human T and non-T lymphocytes.This property is usually maintained after the poison is disabled at 60°C for 10 minutes but in return, its hemolytic activity is reduced (Adam Bownik et al., 2008).