Study Bacteriocin Production and Optimization Using New Isolates of &amp;lt;i&amp;gt;Lactobacillus&amp;lt;/i&amp;gt; spp. Isolated from Some Dairy Products under Different Culture Conditions

Mahrous, Hoda; Mohamed, Abeer A.; El-Mongy, Mahmoud Abd; El-Batal, Ahmed I.; Hamza, Hanafy A.

doi:10.4236/fns.2013.43045

Cited by 38 publications

(38 citation statements)

References 17 publications

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“…LAB isolates grown in media without supplementation could not produce bacteriocins. ISSN 1978-3477, eISSN 2087 *Corresponding author: Phone: +62-24-8441555, Email: laksmi@unika.ac.id often regulated by some factors, such as growth pH, temperature, and nutrient sources (Mahrous et al 2013;Todorov and Dicks, 2005;Zhou et al 2014). Bacteriocin production requires suitable culture medium containing all essential nutrients in suitable amounts.…”

Section: The Effect Of Carbon and Nitrogen Supplementation On Bacterimentioning

confidence: 99%

The Effect of Carbon and Nitrogen Supplementation on Bacteriocin Production of Lactic Acid Bacteria from Pickled Yellow Bamboo Shoots (Dendrocalamus asper)

2018

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Six selected lactic acid bacteria (LAB) isolates from pickled Yellow Betung bamboo shoots were grown in the Mann Rogosa Sharpe-Broth (MRSB) media with different supplementation combination. The cell-free supernatant were evaluated for their ability to produce bacteriocin by adjusting its pH to 6.0 in order to remove organic acid effects. The bacteriocin activity was assayed by agar-well diffusion method. The inhibitory activity 2 -1 calculated in Activity Unit (AU in mm mL ) of bacteriocins. The aims of this paper is to explore the effect of different medium compositions on bacteriocin production and its inhibitory activity against pathogenic bacteria (Listeria monocytogenes FNCC 0156, Staphylococcus aureus FNCC 0047, and Escherichia coli FNCC 0091). Supplementations of carbon and nitrogen sources induced production of bacteriocins. LAB isolates grown in media without supplementation could not produce bacteriocins. Growth of isolate D44 in the presence of 2% of -1 glucose and 2% of yeast extract yielded the largest bacteriocin inhibitory activity levels of 3179 AU mL against -1 Listeria monocytogenes FNCC 0156, 4663 AU mL against Staphylococcus aureus FNCC 0047, and 3109 AU -1 mL against Escherichia coli FNCC 0091. Key words: bacteriocin, lactic acid bacteria, pickled bamboo shoots, supplementation Enam isolat bakteri asam laktat terpilih (LAB) yang diisolasi dari acar rebung bambu kuning ditumbuhkan di media Mann Rogosa Sharpe-Broth (MRSB) dengan kombinasi suplementasi yang berbeda. Supernatan bebas sel diatur pH menjadi 6,0 untuk menghilangkan efek asam organik dan dievaluasi aktivitas bakteriosinnya. Aktivitas bakteriosin diuji dengan metode difusi agar-well. Aktivitas penghambatan dihitung dalam Unit Aktivitas (AU 2 -1 dalam mm mL ) bakteriosin. Tujuan dari penelitian ini adalah untuk mengeksplorasi pengaruh komposisi medium yang berbeda pada produksi bakteriosin dan aktivitas penghambatannya terhadap bakteri patogen (Listeria monocytogenes FNCC 0156, Staphylococcus aureus FNCC 0047, dan Escherichia coli FNCC 0091). Suplementasi sumber karbon dan nitrogen menginduksi produksi bakteriosin. Isolat LAB yang tumbuh di media tanpa suplementasi tidak dapat menghasilkan bakteriosin. Pertumbuhan isolat D44 dengan adanya 2% glukosa -1 dan 2% yeast extract menghasilkan aktivitas penghambatan bakteriosin terbesar, yaitu 3179 AU mL terhadap -1 Listeria monocytogenes FNCC 0156, 4663 AU mL terhadap Staphylococcus aureus FNCC 0047, dan 3109 AU -1 mL terhadap Escherichia coli FNCC 0091. Kata kunci: acar rebung, bakteriosin, bakteri asam laktat, suplementasi MICROBIOLOGY INDONESIA

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Section: The Effect Of Carbon and Nitrogen Supplementation On Bacterimentioning

confidence: 99%

The Effect of Carbon and Nitrogen Supplementation on Bacteriocin Production of Lactic Acid Bacteria from Pickled Yellow Bamboo Shoots (Dendrocalamus asper)

2018

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“…The optimization of bacteriocin production and enhancement of its activity are economically important due to the cost concerns (Mahrous et al 2013). Medium composition and fermentation conditions influence the cell growth and accumulation of metabolic products (Li et al 2002).…”

Section: Introductionmentioning

confidence: 99%

A Sequential Statistical Approach Towards an Optimized Production of Bacteriocin byLactobacillus pentosusTSHS

Motahari

Mirdamadi

Rad

2016

Journal of Food Processing and Preservation

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Bacteriocins, antimicrobial compounds, display applications from food preservation to medicine. The aim of this study was the statistical optimization of a bacteriocin substance production from traditional cheese bacterium taxonomically affiliated as Lactobacillus pentosus TSHS. The effects of variables were first investigated using the Placket–Burman design. Central composite design was then used to compute incubation time and NaCl concentration optimum. Response surface methodology (RSM) revealed that maximum bacteriocin production was achieved at low NaCl concentration (0.13 mol/L) and an incubation time of 47 h. The validation experiments were implemented under optimal conditions with five replicates and the bacteriocin inhibitory activity was increased by three times. The research led to parameters that maximized pentocin activity, which can be expressed as a quadratic function of incubation time and NaCl concentration. The application of RSM resulted in an enhancement in pentocin production cost effective medium. Practical Applications Bacteriocins produced by lactic acid bacteria have been found to inhibit the growth of many foodborne microorganisms, and hence, can be used as natural preservatives to increase shelf life of food. High bacteriocin production is required for application in food industry. However, bacteriocin production does not always correlate with growth rate of bacteriocin producing strain. This study has developed appropriate conditions to give optimum yield of bacteriocin. So, it revealed the possibility of bacteriocin application as biopreservatives according to industrial point of view.

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“…Serially diluted soil sample was allowed to grow on the MRS agar plate. MRS agar is a selective medium containing sodium acetate which suppresses the growth of other competing bacterial group and favours the growth of lactic acid bacteria group and Bacillus species [18]. In preliminary selection, S. epidermidis was used as an indicator organism which is an opportunistic pathogen reported for causing infection.…”

Section: Molecular Characterization Of Potential Candidatementioning

confidence: 99%

Identification and Molecular Characterization of Bacteria Having Antimicrobial and Antibiofilm Activity

Sharma

Dang

Gupta

et al. 2016

Int J Pharm Pharm Sci

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Objective: The aim of the current study was to isolate and identify the bacteriocinogenic strain exhibiting broad range antimicrobial activity and antibiofilm activity from the soil of animal farms.Methods: In the current study, bacterial strains were isolated from soil of twelve different regions of the animal farm all over India and screened for antimicrobial activity against Staphylococcus epidermidis, Micrococcus luteus, Pseudomonas fluorescence and Escherichia coli. Antibiofilm ability of these selected strains was checked on preformed biofilm of S. epidermidis and in addition biofilm disruption potential was also determined. The potent bacterial strain was identified at the molecular level by 16S ribosomal DNA (rDNA) sequencing.Results: 30 out of 231 strains isolated from soil were selected on the basis of antibacterial activity against S. epidermidis. One potential candidate (GAS 101) exhibited ≥99% inhibition against S. epidermidis, M. luteus, P. fluorescence and E. coli and also showed antibiofilm activity. GAS 101 16S rDNA sequencing data identified it as Bacillus subtilis. The sequence of B. subtilis was submitted to genbank under accession no. KJ564301. Conclusion:B. subtilis GAS 101 isolated from soil of animal farm showed the antibacterial activity against all indicator organisms and also displayed antibiofilm activity against preformed biofilm and inhibited biofilm formation of S. epidermidis.

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Study Bacteriocin Production and Optimization Using New Isolates of <i>Lactobacillus</i> spp. Isolated from Some Dairy Products under Different Culture Conditions

Cited by 38 publications

References 17 publications

The Effect of Carbon and Nitrogen Supplementation on Bacteriocin Production of Lactic Acid Bacteria from Pickled Yellow Bamboo Shoots (Dendrocalamus asper)

The Effect of Carbon and Nitrogen Supplementation on Bacteriocin Production of Lactic Acid Bacteria from Pickled Yellow Bamboo Shoots (Dendrocalamus asper)

A Sequential Statistical Approach Towards an Optimized Production of Bacteriocin byLactobacillus pentosusTSHS

Identification and Molecular Characterization of Bacteria Having Antimicrobial and Antibiofilm Activity

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